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Effect of Natural Dye Construction on Antibacterial Activity
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K Kadm Shanshal *    |
| 1.Department of Science, College of Basic Education, Mustansiriyah University, Baghdad, Iraq. , Katreen.kadm@uomustansiriyah.edu.iq |
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Abstract: (14 Views) |
Background and Objective: Natural dyes have attracted much attention due to their antibacterial properties and various applications, especially in textiles, and also due to the environmental pollution of synthetic dyes. The aim of this study is to determine the antibacterial properties and effectiveness of natural dyes by analyzing common dyes against common bacterial strains.
Methods: In this laboratory study, sufficient extracts of natural dyes (Hibiscus Sabdariffa, Nigella sativa, and Lawsonia Inermis) were created for analysis. The dried plant components were pulverized, followed by adding 2g of the resulting dry plant powder of each plant to 100 ml of distilled water, and boiling for 15 minutes at 60◦C. The extract was then strained using a muslin cloth and filter paper. A standard Agar Well Diffusion method determined the initial effects on antibacterial properties.
Findings: The antibacterial activity of three aqueous plant extracts (Hibiscus Sabdariffa, Nigella sativa, Lawsonia Inermis) showed that each sample had a wide range of effects against bacteria from this experiment. Nigella sativa and Hibiscus Sabdariffa extracts had strong antibacterial activity against all bacteria in this study, while Lawsonia Inermis extract had weak antibacterial activity. It appeared from the well diffusion technique that Nigella sativa seeds inhibited E. coli and the inhibition zone was 20 mm and after diluting the concentration, the inhibition zones were 22 and 31 mm at 25 and 50%, respectively, with no inhibition zone at 75% concentration. Regarding Hibiscus Sabdariffa, the inhibition zone was 13 mm against staphylococcus aureus at 100%, and inhibition zone was 12 mm against klebsiell Sp., while the inhibition zone for Nigella sativa for the same bacteria was 11 mm. The Lawsonia Inermis extracts had no inhibition zone for all bacteria. In general, all extracts showed moderate antibacterial activity against bacteria and fungi. The extracts were characterized using Fourier transform infrared spectroscopy (FTIR), UV-Vis spectroscopy and atomic force microscopy (AFM).
Conclusion: The results of the study showed that plant extracts, due to their antibacterial properties against different species, can be effective in treating infections caused by resistant bacteria. |
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| Keywords: Green Technology, Hibiscus Sabdariffa, Nigella Sativa, Antibacterial Activity. |
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Type of Study: Research |
Subject:
Laboratory Sciences
Received: 2024/06/29 | Accepted: 2024/08/26
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