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This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 
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:: Volume 20, Issue 6 (6-2018) ::
J Babol Univ Med Sci. 2018; Volume 20 Back to browse issues page
Preparation and Evaluation of Rivax Protein Loading in Chitosan Nanoparticles
D Sadeghi , F Ebrahimi * , M Zeinoddini , Y Tarverdizadeh , M Bakhshi , MJ Bageripor , SM Agaii
1. Biology Research Center, Faculty of Basic Sciences, Imam Hossein University, Tehran, I.R.Iran , febrhimi@ihu.ac.ir
Abstract:   (3893 Views)
BACKGROUND AND OBJECTIVE: Ricin toxin is a heterodimer glycoprotein which, due to its high toxicity, is used as a bioterrorism agent. Immunogenicity studies against ricin are now focused on two subunit vaccine candidates, including RiVax and RVEc. These studies have examined the vaccine candidate immunization as an alone and in combination with adjuvant, however, there is not a published study on the immunogenicity evaluation of the candidate vaccine through the delivery by nanoparticles. The aim of this study was preparation and evaluation of RiVax recombinant vaccine-loading in chitosan nanoparticles.
METHODS: In this experimental study, After transferring the RiVax gene to the bacterium, inducing the expression and purification of the RiVax protein by affinity chromatography column, the RiVax protein was loaded with
Ionic Gelation method in chitosan nanoparticles. Then, the properties of nanoparticles including size, morphology, loading percentage and release pattern of RiVax protein from nanoparticles and stability of this protein during acidic loading conditions in nanoparticles by SDS-PAGE were evaluated. Also, Immunization study were performed on 3 mouse groups (n=4/group) by RiVax protein, Nanoparticles containing protein and phosphate buffer.
FINDING: The results of this study showed that the nanoparticles containing protein had a size of 178 nm and a Zeta potential of +27.8 MV and a polydispersity index of 0.193. Also, according to SDS-PAGE results, it was found that the RiVax recombinant protein was denatured during the process of preparing the chitosan nanoparticles.
CONCLUSION: The results of this study showed that the RiVax protein has been unstable in acidic conditions for the production of chitosan nanoparticles and
Ionic Gelation method is not suitable for loading this protein in chitosan nanoparticles.
Keywords: Chitosan, Nanoparticles, RiVax, Vaccine
Full-Text [PDF 307 kb]   (1277 Downloads)    
Type of Study: Experimental | Subject: Nanobiotechnology
Received: 2017/12/25 | Accepted: 2018/07/11 | Published: 2018/07/23
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Sadeghi D, Ebrahimi F, Zeinoddini M, Tarverdizadeh Y, Bakhshi M, Bageripor M et al . Preparation and Evaluation of Rivax Protein Loading in Chitosan Nanoparticles . J Babol Univ Med Sci 2018; 20 (6) :62-69
URL: http://jbums.org/article-1-7429-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 6 (6-2018) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

The Journal of Babol University of Medical Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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