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:: Volume 18, Issue 12 (12-2016) ::
JBUMS 2016, 18(12): 19-25 Back to browse issues page
Effect of Ethyl Acetate Extract of Sea Pen Virgularia Gustaviana on Viability of Cancer Cells
Sh Sharifi, P Ghavam mostafavi, A Mashinchian Moradi, M Givian rad, R Tarasi, M Zolghad, H Niknejad
Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran , niknejad@sbmu.ac.ir
Abstract:   (3216 Views)

BACKGROUND AND OBJECTIVE: Due to high cancer incidence rate and become resistant to chemical drugs and their side effects make it necessary to research on new natural compounds. Sea pen with special chemical compounds with anti-cancer effects have been considered in recent years. In this study, extraction of chemical compounds from marine sea pen Virgularia gustaviana and their effect on cancer cells were investigated.

METHODS: In this study the ethyl acetate extract of Virgularia gustaviana was separated by silica gel column chromatography. The column was washed with N-hexane 100% and N-hexane-ethyl acetate solvent at ratio of 9:1 to 1:9. Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) was used for qualitative identification of seven fractions. Viability of HeLa cancer cells was investigated using MTT assay at the concentration of 25, 50 and a 100 µl/ml compounds.

FINDINGS: MTT assay showed that G fraction, dose-dependently decreased cell viability of cells and the most effective concentration was 100 µl (with viability 6.33±2.02% of cancer cells) which was significantly less than control group (p<0.05). Retention time of G fraction in HPLC graph was similar to Cembrane Diterpene isolated from Sarcophyton. 

CONCLUSION: The results of the studyshowed that compounds extracted from Virgularia gustaviana inhibit the growth of cancer cells and further research will be required to examine the mechanism of effect

Keywords: Hela cancer cells, Virgularia gustaviana, Sea pen, Ethyl acetate, Anti-cancer, Cembrane Diterpene
Full-Text [PDF 207 kb]   (987 Downloads)    
Type of Study: Experimental | Subject: pharmacology
Received: 2016/05/30 | Accepted: 2017/01/14 | Published: 2017/01/29
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Sharifi S, Ghavam mostafavi P, Mashinchian Moradi A, Givian rad M, Tarasi R, Zolghad M et al . Effect of Ethyl Acetate Extract of Sea Pen Virgularia Gustaviana on Viability of Cancer Cells. JBUMS. 2016; 18 (12) :19-25
URL: http://jbums.org/article-1-6131-en.html


Volume 18, Issue 12 (12-2016) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

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