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Evaluation and Validation of a New Sample Collection Kit Based on RT-PCR for the Detection of Coronavirus (SARS-COV-2)
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RN Jawad   , HK Abdul-Sada * |
| 2.Al-Zahraa College of Medicine, University of Basrah, Basrah, Iraq. , Hussein.abdul-sada@uobasrah.edu.iq |
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Abstract: (20 Views) |
Background and Objective: During the COVID-19 crisis, all countries faced a major challenge in providing resources to isolate the new corona virus, which was due to lack of required materials. A new cough kit has recently been developed that is easier to use than a nasopharyngeal or pharyngeal swab. The aim of this study is to evaluate and validate a new sample collection kit based on RT-PCR for detection of corona virus (SARS-COV-2).
Methods: In this cross-sectional study, the sputum of 40 patients and 20 healthy individuals were collected through a new sample collection kit for coronavirus (SARS-COV-2) and nasopharyngeal swab. The kit consists of a conical sterile paper mask that covers the patient's mouth and nose. The conical filter contains a polyester polymer swab to collect coughed sputum droplets for sputum extraction. The swab is separated from the cone and placed in the virus transmission medium. The results based on RT-PCR for detection of corona virus (SARS-COV-2) in two methods of sample collection were analyzed and compared.
Findings: The new sample collection kit was able to isolate SARS-COV-2 by RT-PCR from sputum and saliva through cough. The results were positive in 32 of 40 (80%) patients using the cough kit and 21 of 40 (52.5%) samples were positive with nasopharyngeal swab examination. The number of samples that were positive using both methods was 16 (40%). The number of samples that were negative by examining nasopharyngeal swabs and positive using cough swabs was 16 samples (40%). This indicates that cough swabs were 40% more accurate than nasopharyngeal swabs.
Conclusion: Based on the results of this study, the new kit can be used to isolate the SAS-COV2 virus in an infected person instead of a nasopharyngeal swab. |
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| Keywords: COVID-19, Cough, Sputum, Mask Kit. |
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Type of Study: Research |
Subject:
Epidemiology
Received: 2024/09/9 | Accepted: 2024/11/17
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