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This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 
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:: Volume 19, Issue 5 (5-2017) ::
J Babol Univ Med Sci. 2017; Volume 19 Back to browse issues page
Apoptotic Effect of Phosphatidylinositol 3-Kinase Inhibition on Acute Lymphoblastic Leukemia Cells Using Buparlisib
MR Sadri , A Safaroghli Azar , AR Kazemi , M Hamidpour , M Allahbakhshian Frasani , D Bashash *
Department of Hematology and Blood banking, Faculty of Allied Medicine sciences, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran , David_5980@yahoo.com
Abstract:   (5480 Views)

BACKGROUND AND OBJECTIVE: Resistance to chemotherapy is one of the most important problems in treatment of patients diagnosed with acute lymphoblastic leukemia (ALL). Pathway interruption of the phosphatidylinositol -3 kinase (PI3K) and its relation to resistance phenomena cause the inhibitors of this pathway, particularly buparlisib are introduced as one of the most promising cancer drugs. The aim of this study was to evaluate the effect of PI3K pathway inhibition on reducing the survival and induction of apoptosis in Nalm-6 cells using buparlisib.

METHODS: In this experimental study, the phosphorylation level of Akt was evaluated using western blot to measure the effect of buparlisib on PI3K/Akt pathway in Nalm-6 cells. Nalm-6 cells were treated with different concentrations of buparlisib (0.5-4 µM) for 24, 36 and 48 hours to study the cytotoxic effect of this inhibitor and then, the metabolic activity, induction of apoptosis and changes in expression of genes involved in apoptosis were evaluated using MTT assay, Annexin/PI staining and Rq-PCR, respectively.

FINDINGS: Results showed that PI3K pathway inhibition using buparlisib causes the cytotoxic effect on Nalm-6 cells in a dose- and time-dependent manner through reducing p-Akt. These findings suggested that probably, the anti-leukemic effect of buparlisib is mediated through almost 17-fold increase in apoptotic cells (p≤0.001) and rising the mRNA expression level of pro-apoptotic genes (p≤0.01).

CONCLUSION: The results indicated that buparlisib has anti-tumor activity against Nalm-6 cells so this inhibitor can be used as a promising agent for the treatment of ALL.

Full-Text [PDF 421 kb]   (2859 Downloads)    
Type of Study: Experimental | Subject: Hematology
Received: 2017/01/17 | Accepted: 2017/05/15 | Published: 2017/06/7
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Sadri M, Safaroghli Azar A, Kazemi A, Hamidpour M, Allahbakhshian Frasani M, Bashash D. Apoptotic Effect of Phosphatidylinositol 3-Kinase Inhibition on Acute Lymphoblastic Leukemia Cells Using Buparlisib . J Babol Univ Med Sci 2017; 19 (5) :7-13
URL: http://jbums.org/article-1-6586-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 19, Issue 5 (5-2017) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

The Journal of Babol University of Medical Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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