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The Level of Anti-Oxidative Stress Marker Sestrin in Sera and the Expression of SESN1, 2, 3 Genes in T2DM with Dyslipidemia
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E Kamil Raham *   , A Mohsin , A Salim Mohammed |
| 1.College of Health and Medical Technologies, Middle Technical University, Iraq. , 199enk@gmail.com |
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Abstract: (56 Views) |
Background and Objective: Dyslipidemia is an important agent in Diabetes. Sestrin is considered an anti-oxidative stress that affects the metabolism homeostasis, and works as an anti-aging molecule through the regulation of AMPK‐mTORC1 signalling. The aim of this study was to estimate the concentration of sestrin2 protein in patient sera, compared with control, and to measure the expression of SESN gene that encoded to 1, 2, and 3 isoforms.
Methods: This cross-sectional study involved 50 patients with Type 2 Diabetes mellitus (T2DM) and 50 healthy subjects. The clinical diagnoses of the patients included hyperglycemia with and without hyperlipidemia. The detection of them was performed by measuring HbA1C, FBS, and lipid profile. The expression of SESN1, 2, and 3 genes were evaluated by using the relative method.
Findings: The results suggest that the likelihood of developing Type 2 Diabetes Mellitus (T2DM) rises as individuals grow older, with a majority of patients being above 50 years old, which accounts for 52% of the population. The level of sestrin2 protein concentrations were decreased with T2DM and T2DMDL which recorded 11.18 and 12.23, respectively, when compared with control group (29.54). The expression of SESN genes was suppressed with T2DM, the gene SESN1 was recorded 0.801-fold in patients T2DM, 0.514-fold in those with dyslipidaemia (T2DMDL), and SESN2 gene was recorded 0.76-fold in patients suffering from T2DM and 0.70-fold with T2DMDL, the gene SESN3 was recorded 0.69 in T2DMDL, while in T2DM was upregulated to 1.31. The fold-change decreased with the worsening prognosis of the disease. The SESN gene expressions in the patient group were significantly lower than the control group (p≤0.01).
Conclusion: The results of the present study demonstrated that the gene expression was lower in the control group, but regarding SESN3, it was higher in patients with T2DM. On the other hand, it was downregulated in T2DMDL. |
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| Keywords: Diabetes, Sestrin, Dyslipideamia, ELISA, Gene Expression. |
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Type of Study: Research |
Subject:
Nanobiotechnology
Received: 2024/03/31 | Accepted: 2024/06/1
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