[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 20, Issue 6 (6-2018) ::
JBUMS 2018, 20(6): 36-40 Back to browse issues page
The Effect of Tricine Compound Extracted from Allium atroviolaceum Boiss. On growth and apoptosis in PC3 cell line
Z Lorigooini , S Ghasemi , N Asgarian Dehkordi , M Motamedi
2. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, I.R.Iran , sorayya.ghasemi@gmail.com
Abstract:   (756 Views)
BACKGROUND AND OBJECTIVE: Tricine, as a flavonoid compound in many food sources, has anti-inflammatory and anti-proliferative effects in some cancer cell lines. Considering the importance of using natural anti-cancer drugs in therapy-resistant cancers such as prostate cancer, the aim of this study is to investigate the effect of tricine on cell growth and proliferation and induction of apoptosis in PC3 human prostate cancer cell line.
METHODS: In this experimental study, the PC3 cell line was prepared and cultured from the Pasteur Institute of Iran. Extraction and purification of tricine were done by column chromatography and recrystallization of Allium atroviolaceum extract. The apoptotic effect of tricine at concentrations of 60, 80, 100, 120 and 140 μM was evaluated by MTT method. The apoptotic effect was evaluated in the cell group treated with IC50 concentration of tricine and untreated cells (control group) using Annexin-V kit and flow cytometry.
FINDINGS: The viability of cells at different tricine concentrations were 85.66±1.52, 76±3.60, 66.33±4.16, 44±3.60, and 36.66±3.21, respectively (p<0.01). The IC50 concentration of tricine was 117.5±4.4 μM for PC3 cell line. The apoptosis rate in PC3 cells after 48 hours of treatment with IC50 concentration of tricine was 24.3±0.58%, which was not significant in comparison with control cells (23.3±0.58%). 
CONCLUSION: The results of this study showed that tricine resulted in cell death in the PC3 cell line, but the cell death mechanism was not apoptotic.
Keywords: Prostate Cancer, Tricine, Apoptosis
     
Type of Study: Experimental | Subject: J
Received: 2017/10/4 | Accepted: 2018/04/17 | Published: 2018/05/13
Send email to the article author


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Lorigooini Z, Ghasemi S, Asgarian Dehkordi N, Motamedi M. The Effect of Tricine Compound Extracted from Allium atroviolaceum Boiss. On growth and apoptosis in PC3 cell line. JBUMS. 2018; 20 (6) :36-40
URL: http://jbums.org/article-1-7177-en.html


Volume 20, Issue 6 (6-2018) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

The Journal of Babol University of Medical Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Persian site map - English site map - Created in 0.06 seconds with 33 queries by YEKTAWEB 3742