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:: Volume 18, Issue 3 (3-2016) ::
JBUMS 2016, 18(3): 25-32 Back to browse issues page
Increased Toxicity of Chemotherapeutic Drugs by All-Trans Retinoic Acid in Cd44 Cells
A Abbasi *, M Mazani, N Najafzadeh, M Amani, H Sheykhkanlooy Milan
, asadallahabbasi@gmail.com
Abstract:   (4144 Views)

BACKGROUND AND OBJECTIVE: In recent studies, undifferentiated CD44 cells have been introduced as the major cause of chemotherapeutic drug resistance in esophageal cancer. In this study, we aimed to evaluate the effects of all-trans retinoic acid on reducing chemotherapeutic drug resistance and improving the associated toxic effects.

METHODS: In this clinical study, CD44+ and CD44- cells were separated from KYSE-30 cell line, using magnetic-activated cell sorting (MACS) method. The cytotoxic effects of retinoic acid treatment, combined with cisplatin and 5-fluorouracil, were separately evaluated in two cell groups, i.e., CD44+ and CD44-. Cytotoxicity was determined by identifying cellular metabolic activity, acridine orange/ethidium bromide staining, and flow cytometry.

FINDINGS: In this study, CD44 marker was expressed in 6.25% of the cell population in KYSE-30 cell line. The results of flow cytometry revealed that treatment with a combination of retinoic acid and chemotherapeutic drugs could improve cell cycle arrest in CD44+ cells (p<0.05), unlike CD44- cells. Determination of cellular metabolic activity, increased cell apoptosis along with decreased half maximal inhibitory concentration (IC50), and acridine orange/ethidium bromide staining were indicative of the increased percentage of primary and secondary apoptotic CD44+ cells. However, in CD44- cells, these effects were only observed by using a combination of retinoic acid and cisplatin (p<0.05).

CONCLUSION: The present results showed that all-trans retinoic acid could increase the toxicity of cisplatin and 5-fluorouracil in CD44+ cells.

Keywords: KYSE-30 cell line, Retinoic acid, Cisplatin, 5-fluorouracil, CD44, Chemotherapy
Full-Text [PDF 509 kb]   (1024 Downloads)    
Type of Study: Experimental | Subject: Biochemical
Received: 2014/10/7 | Accepted: 2015/05/24 | Published: 2016/03/7
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Abbasi A, Mazani M, Najafzadeh N, Amani M, Sheykhkanlooy Milan H. Increased Toxicity of Chemotherapeutic Drugs by All-Trans Retinoic Acid in Cd44 Cells. JBUMS. 2016; 18 (3) :25-32
URL: http://jbums.org/article-1-5081-en.html

Volume 18, Issue 3 (3-2016) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

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