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Simultaneous Effect of Resveratrol and Probiotics on Insulin Resistance and Glucagon-Like Peptide (GLP-1) Levels in Diabetic Rats
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A Pegah    , I Khodadadi , F Mirzaei , H Tayebinia , E Abbasi * |
| 1. Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, I.R.Iran , 7abbasi@gmail.com |
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Abstract: (2806 Views) |
BACKGROUND AND OBJECTIVE: The main and effective treatment for diabetes is the use of insulin and synthetic drugs and herbal medicines, which may be accompanied by several side effects. Given that GLP-1 secretion is reduced in diabetic patients compared to healthy individuals, the aim of this study was to determine the simultaneous effect of resveratrol and probiotics on insulin secretion and glucagon-like peptide (GLP-1) and to evaluate insulin resistance in diabetic rats.
METHODS: In this experimental study, 40 adult male Wistar rats weighing approximately 250-300 g were used. Animals were randomly divided into 5 groups: control group, diabetic group (using 65 mg/kg streptozotocin and 110 mg/kg nicotinamide intraperitoneally), diabetic group treated with probiotics (50×109 bacteria/kg body wt−1 daily by dissolving in water), diabetic group treated with resveratrol (10 mg/kg by gavage method) and diabetic group treated with probiotics and resveratrol (co-administration group). After four weeks, the animals were sacrificed and pancreatic tissue and blood samples were isolated for histopathological tests and biochemical tests. Blood glucose, insulin resistance, pancreatic GLP-1 as well as pancreatic histopathology were assessed.
FINDINGS: The co-administration group compared with the diabetic group caused a 42% reduction in serum glucose levels (p<0.001) and a 30% reduction in insulin resistance (p<0.001). The co-administration group also caused a 23.5% increase in insulin levels (p<0.001) and an 88% increase in GLP-1 (p<0.05). The co-administration group improved the histology of the pancreas.
CONCLUSION: The results of the study showed that resveratrol and probiotics are effective in controlling diabetes by increasing the levels of GLP-1 and insulin and decreasing insulin resistance. |
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| Keywords: Probiotics, Resveratrol, Type 2 Diabetes, Glucagon-Like Peptide (GLP-1). |
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Full-Text [PDF 607 kb]
(788 Downloads)
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Type of Study: Experimental |
Subject:
Biochemical
Received: 2020/10/12 | Accepted: 2021/05/19 | Published: 2021/11/9
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