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Effect of Visceral Fat on the Amount of Hydroxyproline and Histopathological Changes on Surgical Wound Healing in Male Rat
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K Didevar *    , S H Davoodi , Gh Javdan |
| 1. Department of Biochemistry, Alborz University of Medical Sciences, Karaj, I.R.Iran , kdidevar8@gmail.com@gmail.com |
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Abstract: (3200 Views) |
BACKGROUND AND OBJECTIVE: expensive treatments and rehabilitation has increased the tendency to use natural and cheaper compounds. Animal visceral lipids contain a high percentage of different types of effective anti-inflammatory fatty acids, which till now have not been studied together. Therefore, the purpose of this study was to evaluate the anti-inflammatory and regenerative effects of sheep visceral lipids on surgical wound healing in rat rats.
METHODS: In this experimental study 50 Sprague-Dawley adult male rats with the average weight of 250-300 g were selected and randomly divided into 2 groups with 25 rats in each group. After providing visceral fat from the pre renal part of the young sheep and melting and sterilizing them, the treatment group which were wounded under surgical anesthesia by excisional wounds, were treated by 10 ml of it on the wound each day. The evaluation of the wound size was measured by using an accurate caliper and Image J software on days 5, 10, 12, 15. Tissue biopsies from both control and treatment groups were performed to compare the rate of epithelialization, collagen filament formation, inflammatory cells, vascularization, and fibroplasia in situ. Also a biopsy was performed at day 21th to check the amount of hydroxyproline in the tissue.
FINDINGS: Based on morphometric findings, on the 10th day of treatment, the mean ulcer size of the treatment group with visceral fat was 43.6±0.44 mm2 while the mean size of the ulcer in control group was 98.5±0.07 mm2, which has shown to have the least amount of wound contraction. Also on the 5th day, the wound contraction of the treatment group, was twice as high as the control group(p<0.05). The histological examination shows the mean 2.74±0.17 points of restoration parameters score in treatment group compared to the control group with a score of 1.42±0.16 points. Also, the difference between the Hydroxyproline concentration of the healthy skin and the scarring wounds in treatment group (3.69±0.23 mg/g) was less than the control group(6.08±0.32 mg/g) (p<0.05).
CONCLUSION: According to the findings of this study, the usage of animal visceral lipid, with anti-inflammatory effects and as a stimulator of collagen production, increases the amount of hydroxyproline and affects the healing process of cutaneous ulcers. It also improves the blood perfusion and angiogenesis by increasing RBC membrane flexibility, which will also enhance the migration of cells that are effective in wound healing. |
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| Keywords: Wound, Visceral Fat, Rat, Fatty Acid, Hydroxyprolin |
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Full-Text [PDF 563 kb]
(1041 Downloads)
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Type of Study: Experimental |
Subject:
Skin
Received: 2019/06/4 | Accepted: 2019/09/18 | Published: 2019/12/1
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