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JBUMS 2019, 21 - : 7-0 Back to browse issues page
Identification and Molecular Characterization of Streptococcus pneumoniae Serotypes From Selected in Tehran Hospitals by Multiplex PCR
S RafieiTabatabaei , F Fallah , D Afshar , A Nazari Alam
, nazarialam-a@kaums.ac.ir
Abstract:   (140 Views)
BACKGROUND AND OBJECTIVE: Streptococcus pneumoniae is a most important pathogen that leads to many invasive diseases including pneumonia, and meningitis. Epidemiological study of pneumococcal serotypes in a community is used to evaluate pneumococcal vaccine efficacy.  Hence, the present study was aimed to Identification and molecular characterization of Streptococcus pneumoniae serotypes by multiplex PCR isolated from selected hospitals in Tehran.
METHODS: In this descriptive cross-sectional study, a number of 32 isolates was obtained from clinical specimens submitted to different hospitals in Tehran. Isolates were identified using phenotypic and PCR tests. Multiplex PCRs was used to determine serotypes of isolates.
FINDINGS: The number and percentage of pneumococcal strains isolated from cerebrospinal fluid (CSF), sputum, blood cultures, BAL, eye and nasal discharge specimens were [n = 12 (37.6%)], [n = 7 (21.8%)], [n = 6(18.7%)], [n = 3 (9.5%)], [n = 2 (6.2%)], [n = 2 (6.2%)], respectively. Isolated belonged to serotypes 1, 4, 6A.B, 7F, 9V, 11A, 14, 15A, 19A, 19F, and 23F.
CONCLUSION: According to the results, more than 50% of serotypes did not belong to vaccines that are used in Iran to protect children. Therefore, more studies are needed to ensure that the vaccination is effective and can prevent the onset of pneumococcal disease.
 
Keywords: Streptococcus pneumoniae, Serogroup, Multiplex Polymerase Chain Reaction, Iran.
     
Type of Study: Cross Sectional | Subject: Mi
Received: 2018/05/20 | Accepted: 2019/01/27 | Published: 2019/04/10
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RafieiTabatabaei S, Fallah F, Afshar D, Nazari Alam A. Identification and Molecular Characterization of Streptococcus pneumoniae Serotypes From Selected in Tehran Hospitals by Multiplex PCR. JBUMS. 2019; 21 :7-0
URL: http://jbums.org/article-1-7866-en.html


Volume 21 - Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

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