Lack of Genetic Association between Interleukin-18 Gene Polymorphism (rs1946518) and Chronic Hepatitis B Infection
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M Salehi , SR Mohebbi * , M Karkhane , Sh Kazemian , P Azimzadeh , M Saeedi Niasar , A Sharifian , MR Zali |
2- Gastroenterology and liver diseases research center, Research institute for gastroenterology and liver diseases, Shahid Beheshti university of medical Sciences, Tehran, Iran , srmohebbi@gmail.com |
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Abstract: (3498 Views) |
BACKGROUND AND OBJECTIVE: Interleukin 18 is a member of the cytokines that play an important role in the Th1-mediated immune response by inducing interferon-gamma activity in collaboration with Interleukin-12 (IL-18). Interleukin 12 and Interleukin 18 can play an important role in purifying viruses. Considering the importance of IL-18, this study was conducted to investigate the relationship between Interleukin-18 Gene polymorphism (-607 C/A: rs1946518) and the susceptibility to chronic hepatitis B infection.
METHODS: In this case-control study, the genomic DNA of 115 patients with chronic hepatitis B (with positive results of HBsAg and Anti-HBcAb serology testing) and 115 non-HBV-infected controls (negative results of HBsAg and Anti-HbcAb serology testing and no history of liver disease) was extracted by salting-out method and the genotype of single-nucleotide polymorphism (-607 C / A: rs1946518) was sequenced using PCR-RFLP method.
FINDING: The genotype frequency of TT, GT, and GG in patients was 40%, 49.6%, and 10.4% in patients, and 41.7%, 42.6%, and 15.7% in the control group, respectively. No significant difference was found between the patients group and the control group.
CONCLUSION: Based on the results of this study, there was no clear relationship between IL-18 polymorphism and the potential for chronic hepatitis B infection. Therefore, this polymorphism cannot be a potential factor for chronic hepatitis B. |
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Keywords: Interleukin 18, Chronic Hepatitis, Hepatitis B Virus, Single-Nucleotide Polymorphism |
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Full-Text [PDF 207 kb]
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Type of Study: Case-Control |
Subject:
Virology Received: 2017/12/2 | Accepted: 2018/07/8 | Published: 2018/07/23
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