[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 20, Issue 6 (6-2018) ::
JBUMS 2018, 20(6): 20-26 Back to browse issues page
Evaluating the Performance of Flow Cytometric Method in Identification of Platelet Resistance in Patients with Acute Myeloid Leukemia (AML)
M Ghorbani , MT Hojjati
, mthhematology@gmail.com
Abstract:   (924 Views)
BACKGROUND AND OBJECTIVE: Immune platelet resistance is a condition in which antibodies are produced against platelet antigens and cause damage to injected platelets by phagocytes and macrophages. The aim of this study is to evaluate the efficacy of flow cytometry in predicting the results of platelet injections and platelet resistance and selecting compatible platelet for patients.
METHODS: This case-control study was performed on 15 patients with acute myeloid leukemia (AML) with two or multiple injections of platelets and 15 healthy subjects without a history of platelet injection. After marking the platelets with 5-Chloromethylfluorescein diacetate (CMFDA) and their adjacency to the serum of patients, the amount of phagocytosis of platelets by monocytes was assessed in the two groups. 
FINDINGS: The mean phagocytic power of platelets by monocyte was 18.27 ± 2.86% in the control group, 68.47 ± 10.40% in the group of patients with immune platelet resistance, and 36.73 ± 15.21 in the group of patients without immune platelet resistance (p = 0.001). In addition, there was a significant negative correlation between phagocytic power of platelets by monocyte and Corrected Count Increment (CCI) at 1 and 24 hours (p = - 0.001).
CONCLUSION: Based on the results of this study and considering the high sensitivity of the flow cytometric method, the use of CMFDA is highly appropriate for evaluating platelet studies, and it can also be used for platelet crossmatch by flow cytometry.
Keywords: Platelet Resistance, Flow Cytometry, CCI, Phagocytosis, 5-Chloromethylfluorescein diacetate
     
Type of Study: Case-Control | Subject: Hematology
Received: 2017/11/4 | Accepted: 2018/04/17 | Published: 2018/05/13
Send email to the article author


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghorbani M, Hojjati M. Evaluating the Performance of Flow Cytometric Method in Identification of Platelet Resistance in Patients with Acute Myeloid Leukemia (AML). JBUMS. 2018; 20 (6) :20-26
URL: http://jbums.org/article-1-7284-en.html


Volume 20, Issue 6 (6-2018) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

The Journal of Babol University of Medical Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Persian site map - English site map - Created in 0.06 seconds with 33 queries by YEKTAWEB 3764