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Histological Evaluation of Different Types of Mastocytes in the Skin Flap Using Bone Marrow Mesenchymal Stem Cells Through Biological Growth Factor
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F Chehelcheraghi *   , Kh Anbari |
| 1. Department of Anatomical Sciences, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, I.R Iran. , fr.chehelcheraghi@gmail.com |
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Abstract: (5201 Views) |
BACKGROUND AND OBJECTIVE: Skin flap is one of the most commonly used methods in plastic surgery. Postoperative skin flap necrosis is one of the complications of flap skin. The aim of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BM-MSCs) and chick embryo extract (CEE) on mast cells in a randomized skin flap in rats.
METHODS: In this experimental study, 40 male albino Wistar rats weighing 250 – 300 g were divided into four groups of 10 (control, CEE/BM-MSCs, CEE and BM-MSCs). Skin flap (30 × 80 mm) was created behind the animals. Surgery was performed on day zero and therapeutic intervention was done on the same day. Mesenchymal stem cells were extracted from rat bone marrow and were injected. CEE was prepared from a 9-day-old embryo of Marandi chicken. On the seventh day after the surgery, samples were assessed in term of type, and the total number of mast cells (type 1 to 3) in the transfer line.
FINDINGS: The difference between the mean number of mast cells type 1 (3.67±1.91) (p=0.99), and type 3 (1.9±1.47) (p=0.384) was not significant in the study groups, but was statistically significant in type 2 (2.27±1.42) in different study groups (p=0.005). There was also a statistically significant difference between the mean total number of mast cells (2.32±0.84) in the BM-MSCs group and other experimental groups (p=0.001).
CONCLUSION: Based on the results of this study, increase in mast cell type 2, the improvement of small vessels and decrease in mast cell type 3 lead to the reduction of scarring and fibrosis |
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| Keywords: Biological Growth Factor, Mast Cells, Flap Surgery, CEE. |
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Full-Text [PDF 333 kb]
(1575 Downloads)
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Type of Study: Experimental |
Subject:
Histology
Received: 2017/09/26 | Accepted: 2018/01/24 | Published: 2018/02/13
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