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Investigation of the Relationship between the Presence of Chromosomal and Plasmid-Encoded AmpC Genes and Type of Clinical Specimen in Pseudomonas Aeruginosa
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H Tahmasebi   , MY Alikhani , S Dehbashi , MR Arabestani * |
| 3.Brucellosis Research Center, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, I.R.Iran. , mohammad.arabestani@gmail.com |
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Abstract: (4335 Views) |
BACKGROUND AND OBJECTIVE: Different clinical specimens play a decisive role in the type and nature of drug resistance in pathogenic organisms. Occasionally, the presence of certain antibiotic resistance genes is associated with the type of clinical specimen. The aim of this study was to determine the relationship between the presence of chromosomal and plasmid-encoded AmpC genes and type of clinical specimen in Pseudomonas aeruginosa.
METHODS: In this descriptive and experimental study, 114 isolates of Pseudomonas aeruginosa, and clinical specimens including blood, urine, wound secretion, burn injuries were collected from teaching hospitals in Hamadan. The presence of chromosomal and plasmid-encoded AmpC genes was evaluated using multiplex PCR technique.
FINDINGS: The plasmid-encoded AmpC genes were observed more than chromosomal genes in Pseudomonas aeruginosa isolates. The FOX gene with a value of 29 (37.66%) (p≤0.037) and DHA gene with a value of 5(6.4%) (p≤0.015) in plasmid-encoded AmpC genes, while FOX gene with a value of 39 (48.75%) (p≤0.001) and MOX gene with a value of 2 (7.36%) in chromosomal AmpC genes had the highest and lowest frequency, respectively.
CONCLUSION: The results of the study showed that the presence of chromosomal and plasmid-encoded AmpC genes may have various frequencies according to the type of clinical specimen. |
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| Keywords: Pseudomonas Aeruginosa, Drug Resistance, Ambler Classification of Β-Lactamases, Plasmid, Chromosome |
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Full-Text [PDF 312 kb]
(1375 Downloads)
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Type of Study: Research |
Subject:
Microbiology
Received: 2017/09/16 | Accepted: 2018/03/5 | Published: 2018/03/17
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