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This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 
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:: Volume 19, Issue 9 (9-2017) ::
J Babol Univ Med Sci. 2017; Volume 19 Back to browse issues page
Immunogenicity Evaluation of PLGA Nanoparticles Contains Recombinant CfaB Protein from Enterotoxigenic Escherichia Coli
Sh Nazarian , L Mousavi Gargari *
Department of Biology, Faculty of Basic Sciences Shahed University, Tehran, I.R.Iran , mlatifm@yahoo.com
Abstract:   (4642 Views)

BACKGROUND AND OBJECTIVE: Enterotoxigenic Escherichia coli (ETEC) are the most common causes of diarrhea among children. Colonization factors (CFs) are the major ETEC candidate vaccines. Considering the high antibiotics resistance reports, construction of effective immunogen against bacteria is major goal. Encapsulation of recombinant immunogen in nano carriers such as polymeric PLGA nanparticles, not only protects them from degradation in environmental elements but also provides the effective concentrations of immunogen in targets, hence  increasing bioactivity. The aim of this study was to investigate the immunogenic properties of CfaB protein encapsulated in PLGA nanoparticles.
METHODS: In this research study, cfaB gene codon optimaztion was performed by OPTIMIZER software. Expression of recombinant protein in E.coli was induced with IPTG. The recombinant protein was purified using affinity chromatography and evaluated by Western Blotting analysis. PLGA nanoparticles containing recombinant protein were prepared by double emulsion method and their structures were characterized by SEM and DLS. 40 BALB/C mice in four group were immunized with nanoparticles and antibody titers were determined by ELISA. The efficiency of antibodies in preventing the attachment of ETEC bacteria to the Caco 2 cells was examined.
FINDINGS: The optimized gene had a Codon adaptation index (CAI) of 0.85. Expression of recombinant protein led to the production of CfaB with molecular weight of 18/9 kDa. Yield of protein was 5mg/L. Encapsulation efficiency of recombinant protein in PLGA nanoparticles was 85٪. The average particle size was 170 nm. Immunization of mice induced serum antibody response. Immunized sera decreased %62.8 of ETEC adhesion to Caco2 cells.
CONCLUSION: Recombinant CfaB protein encapsulated in PLGA nanoparticles stimulate immunity against ETEC.

Keywords: PLGA, nanoparticle, ETEC, CfaB, Immunization
Full-Text [PDF 395 kb]   (1135 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2017/04/4 | Accepted: 2017/08/19 | Published: 2017/09/17
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Nazarian S, Mousavi Gargari L. Immunogenicity Evaluation of PLGA Nanoparticles Contains Recombinant CfaB Protein from Enterotoxigenic Escherichia Coli . J Babol Univ Med Sci 2017; 19 (9) :39-44
URL: http://jbums.org/article-1-6715-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 19, Issue 9 (9-2017) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

The Journal of Babol University of Medical Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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