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Introduction and Optimization of a Dietary Model for Inducing Hyperlipidemia in Rats
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A Amirabadizadeh   , M Foaddodini , S Khatamsaz * , M Mokhtari , M Shariati , M Hosseini , F Hajipour |
| Department of Biology, Islamic Azad University, Kazerun Branch, Kazerun, I.R.Iran , Saeed1617@yahoo.com |
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Abstract: (5099 Views) |
BACKGROUND AND OBJECTIVE: Inducing hyperlipidemia in laboratory animals through diet is a good way to study metabolic disorders. This study was conducted to provide an effective and accessible diet for generating hyperlipidemia and non-alcoholic fatty liver models in rats.
METHODS: In this experimental study, 40 male Wistar rats (180-200 g) were divided into 5 equal groups including 2 control groups receiving regular diet for 4 (C1) or 8 weeks (C2), and 3 experimental groups receiving high fat diet along with 0.2% (E1) or 0.1% thiouracil (E2) for 8 and 4 weeks (E3). Finally, the concentration of total cholesterol (TC), LDL, HDL, and triglyceride (TG) was measured and the fat accumulation in the liver tissue was measured quantitatively.
FINDINGS: All experimental groups had significantly higher TC, TG, LDL and lower HDL compared to control (p<0.0001). The cholesterol level was significantly higher in E1 (642.66±133.01), E2 (848.16±146.17) and E3 (406.83±116.28) groups, compared with the C1 (64.87±16.10) and C2 (76.83±11.37) groups (p<0.0001). The degree of fat accumulation in the groups E1 (3.70±0.34), E2 (3.45±0.32) and E3 (2.83±0.25) was significantly higher than the groups C1 (0.25±0.01) and C2 (0.33±0.03) (p <0.0001).
CONCLUSION: The high-fat diet introduced in this study can cause hyperlipidemia and non-alcoholic fatty liver rats within 4 weeks |
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| Keywords: High-fat diet, Hyperlipidemia, Non-alcoholic fatty liver, Rat |
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Full-Text [PDF 386 kb]
(1559 Downloads)
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Type of Study: Experimental |
Subject:
Biochemical
Received: 2017/01/2 | Accepted: 2017/06/25 | Published: 2017/07/30
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