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This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 
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:: Volume 18, Issue 10 (10-2016) ::
J Babol Univ Med Sci. 2016; Volume 18 Back to browse issues page
Molecular Identification of Virulence Genes (agfA and mgtC) in Salmonella Typhimurium Strains Isolated from Children with Gastroenteritis Using Multiplex PCR Method and Determination of Their Antibiotic Susceptibility Pattern
S Amiri , GA Moradli *
Department of Microbiology, Faculty of Sciences, Islamic Azad University of Saveh, I.R.Iran , moradli.mic@gmail.com
Abstract:   (4647 Views)

BACKGROUND AND OBJECTIVE: Gastroenteritis caused by salmonella is common in humans and is regarded as a global public health issue. The aim of this study is to identify agfA and mgtC genes in salmonella typhimurium strains isolated from stool samples using multiplex PCR method and to determine the resistance patterns of these strains.

METHODS: This cross-sectional study was conducted on salmonella typhimurium isolated from children with gastroenteritis admitted to Children's Hospital Medical Center in Tehran. Frequency of agfA and mgtC genes was evaluated using multiplex PCR method. In addition, antibiotic susceptibility of these isolates was studied using gel diffusion method and according to CLSI guidelines.

FINDINGS: Of total 200 stool samples, 60 salmonella typhimurium isolates were obtained. Molecular analysis showed that 24 isolates contained both agfA and mgtC genes at the same time, while 52 isolates contained mgtC gene and 40 isolates carried agfA gene. All strains (100%) were susceptible to ciprofloxacin and 85% of strains were resistant to nitrofurantoin.

CONCLUSION: Results of the study demonstrated that most frequent virulence gene in these strains was mgtC (86.6%) and the least frequent virulence gene was agfA (66.6%). Moreover, it was concluded that these isolates were 100% susceptible to ciprofloxacin and were the most resistant to nitrofurantoin (85%).

Keywords: Salmonella typhimurium, mgtC and agfA genes, Antibiotic susceptibility
Full-Text [PDF 261 kb]   (1687 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2016/05/15 | Accepted: 2016/10/30 | Published: 2016/11/22
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Amiri S, Moradli G. Molecular Identification of Virulence Genes (agfA and mgtC) in Salmonella Typhimurium Strains Isolated from Children with Gastroenteritis Using Multiplex PCR Method and Determination of Their Antibiotic Susceptibility Pattern. J Babol Univ Med Sci 2016; 18 (10) :40-45
URL: http://jbums.org/article-1-6093-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 10 (10-2016) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

The Journal of Babol University of Medical Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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