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Expression of P53 and P63 in Oral Lichen Planus and Oral Lichenoid Lesions
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M Seyedmajidi *   , SH Shafaee , M Hejazi , M Haji Ahmadi , S Siadati |
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Abstract: (7460 Views) |
BACKGROUND AND OBJECTIVE: A few samples of oral lichen planus and lichenoid reactions may be change to malignancy. Histopathological diagnosis is a tool for diagnosis but it is not correct for segregation of these lesions. The aim of this survey was to examine immunohistochemistry expression of P53 and P63 in oral lichen planus and oral lichenoid lesions according to different aetiopathogenesis, clinical behavior and neoplastic changes of these diseases.
METHODS: The cross sectional study was done on 80 cases (40 cases of oral lichen planus and 40 cases of oral lichenoid lesions) gotten from pathology department of Babol dental faculty and Shahid Beheshti hospital. Slices gotten from paraffined block stained by P63 and P53 antibody, using immunohistochemical procedure. The percent of stained cells in basal layer, suprabasal layers and inflammatory inflammation was graded. when none of cells were stained (-) the stained cells were included < 10% of the total cell population (+) when the stained cells were included from 10% to 25% of the total cell population (++) when the stained cells were included from 26% to 50% of the total cell population (+++) and (++++) for the stained cells were accounted >50%. The obtained results were assessed and compared.
FINDINGS: The expression of P53 in oral lichen planus was more than oral lichenoid lesions, (p<0.0001), but there was not significant statistical relation regarding to P63 expression between oral lichen planus and oral lichenoid lesions (p=0.379). Expression of P53 and P63 in two types of oral lichen planus (Reticular, Erosive) did not have significant difference (p>0.05). The expression of P53 in oral lichen planus and lichenoid lesions, in basal layer was more than suprabasal layer and inflammatory infiltration and in suprabasal layer was more than inflammatory infiltration. The same result gotten about P63 expression (p<0.05). The rate of P53 in oral lichen planus, in basal layer (p=0.012) suprabasal layers (p<0.0001) and inflammatory infiltration (p=0.003), was more than oral lichenoid lesions, but there was not significant statistical relation between oral lichen planus and oral lichenoid lesions in expression of P63 in basal layer, suprabasal layer and inflammatory infiltration.
CONCLUSION: The results showed the coordination activity of P63 and P53 proteins to protect oral mucosa from harmful effects of inflammation. |
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| Keywords: Lichen planus, Oral lichenoid lesions, P53, P63, Immunohistochemistry |
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Full-Text [PDF 1218 kb]
(2000 Downloads)
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Type of Study: Research |
Subject:
Biochemical
Accepted: 2014/06/7 | Published: 2014/06/7
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