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KI67 AND P53 PROTEINS IN ODONTOGENIC KERATOCYST
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S Seifi *   , S Mahjoub , E Shafigh, , A Bijani , MH Ahangari |
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Abstract: (8948 Views) |
BACKGROUND AND OBJECTIVE: Ki67 is proliferation marker in proliferative cells and P53 is a tumor suppressor gene. Meanwhile the cause of high recurrence of keratocyst is unknown. The purpose of this study was to evaluate immunostaining reaction of Ki67 (proliferation marker) and P53 (tumor suppressor gene) in different and all layers of odontogenic keratocyst (OKC).
METHODS: This descriptive study was performed on 20 paraffinized blocks. From parafinized blocks, 3-µm section was cutted and then IHC was done for Ki67, P53 markers with anti Ki67 antibody and anti P53 antibody. Nucleus of epithelial cells was immunostained in different layers of OKC in 1000 epithelial cells, in 10 HPF (high power field) were counted and compared to each other.
FINDINGS: Immunostained nucleus of epithelial cells with Ki67 in basal, intermediate and surface layers and all lining epithelium of OKC were 14.3±4.4%, 41.4±12.8%, 0, 18.5±6.1%, respectively. Mean of nucleus immunoreactive epithelial cells with P53 in basal, intermediate, surface layers and all lining epithelium of OKC were 11.7±3.2%, 23.9±7.6%, 0, 11.8±3.6%, respectively. Mean of immunostained nucleus of epithelial cells with Ki67 and P53 markers was statistically significant in different layers of epithelium of OKC (p<0.0001). There was a significant relationship between mean of nucleus immunoreactive epithelial cells with Ki67 and P53 in intermediate layer of OKC.
CONCLUSION: According to the results, high immunoreactivity with Ki67 and P53 markers in intermediate layers compared to basal, surface and in all lining epithelium of OKC showed exclusion of epithelial cells from normal cell cycle and tendency to recurrence and aggressive behavior in OKC. Also, high expression of Ki67 and P53 in intermediate layers could be used to differentiate aggressive of odontogenic cysts from other odontogenic cysts. |
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| Keywords: Ki67 protein, P53 protein, Immunohistochemistry, Odontogenic keratocyst, Cell proliferation |
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Full-Text [PDF 384 kb]
(2188 Downloads)
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Type of Study: Research |
Subject:
Biochemical
Accepted: 2014/06/3 | Published: 2014/06/3
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