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EFFECT OF HEAT ON THE STAPHYLOCOCCI AND E. COLI RESISTANCE TO ANTIBIOTICS
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Z Moulana *   , Z Shahandeh , M Haji Ahmadi |
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Abstract: (10155 Views) |
BACKGROUND & OBJECTIVE: One of the most important problems in treatment of infectious diseases is antibiotic resistance in bacteria. The bacteria can be resistant to antibiotics in different ways which the most common one is the plasmid-origin resistance. Since some plasmids have a kind of replication system which is sensitive to heat and eliminated by growing in unauthorized heat, the objective of this study was to survey the possibility of changing in antibiotic resistant bacteria by heat.
METHODS: In this experimental and interventional study after collecting clinical samples, different tests were done to recognize bacteria completely. For every sample, at first an antibiogram test was done by disk diffusion method. Then bacteria incubated in the BHI broth during 24 hours at 43.5°c, in the next step, bacteria were captured in BHI agar during 24 hours at 35°c and finally the second antibiogram was carried out by disk diffusion. P<0.05 was considered significant.
FINDINGS: Among the staphylococcus, the most changes belonged to oxaciline (11%) which p<0.0001 was considered significant while no changes were seen in rifampicin. Among the E. coli, the most changes belonged to ampicilin (7%) which p<0.0001 was considered significant while no changes were seen in ceftriaxone and ciprofloxacin antibiotics.
CONCLUSION: Heat can cause bacteria to change from antibiotic resistant bacteria to antibiotic semi-sensitive and sensitive bacteria. |
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| Keywords: Escherichia.coli, Staphylococcus, Heat, Resistance to antibiotic |
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Full-Text [PDF 278 kb]
(4627 Downloads)
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Type of Study: Research |
Subject:
Biochemical
Accepted: 2014/06/1 | Published: 2014/06/1
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