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Effects of Serine Enzyme Extracted from Earthworm Lumbricus Terrestris in the Expression of Apoptosis-Related Protein on the MCF-7 Cell Line
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A Abdel Salam Qassem *    , N Faleh , FA Ali Shafi |
| 1.College of Dentistry, Mustansiriyah University, Baghdad, Iraq. , aymansalam@uomustansiriyah.edu.iq |
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Abstract: (7 Views) |
Background and Objective: Cancer is the leading cause of death worldwide, and breast cancer is the most common type of cancer in women worldwide. Earthworms have been used in medicine to cure a variety of diseases because their body extracts have a variety of bioactive substances (such as proteins and peptides). Serine enzyme is an enzyme purified from the earthworm with a significant antitumor activity against human cancer cell. The aim of the study is to determine the mechanisms by which serine proteases induce apoptosis and to examine the expression of a number of apoptosis-associated proteins in treated and untreated (MCF-7) cell line using microarray.
Methods: In this laboratory study, healthy and fully mature earthworms were collected. After seven days of growing in humus-enriched soil with intermittent water sprays in a controlled laboratory setting. Lumbericas terrestris serine protease was added to 1x105 cells/mL of MCF-7 cells after they were seeded, and the treatment lasted for 24 hours at IC50 concentration. Untreated cells were employed as a negative control. Following a 24-hour treatment period, MCF-treated cells were collected and spun down for five minutes at 2500 rpm and 4°C. Serine enzyme was partially purified from earthworms Lumbricus terrestris and the MCF-7 cell line was treated with serine enzyme.
Findings: The results showed that treatment of MCF-7 cells with serine protease led to changes in most apoptosis-related proteins. Treatment of MCF-7 cells with serine protease resulted in downregulation of cIAP-2 (632±7.2 & 275±4.8), Livin (220±4.7 & 95±2.1), XIAP, Survivin, BCLw, BAD, BAX, BID, BIM, cytoC and P53 with an increased expression of caspase 3 (83±1.5 & 493±4.9), caspase 8 (285±3.33 &610±7.65) and SMAC compared to untreated cells.
Conclusion: The results of the study showed that serine protease has the ability to facilitate perforation of mitochondrial membrane as well as caspase 3 cleavage in apoptotic cell death.
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| Keywords: Serine, Earthworm, Mcf-7, Apoptosis Protein, Microarray. |
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Type of Study: Research |
Subject:
Genetics, Cell and Molecular Biology
Received: 2024/11/22 | Accepted: 2024/12/30
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