Background and Objective: Propolis is a hive product composed primarily of beeswax and phytogenic components. It has been recognized for its antibacterial and wound-healing properties since ancient times. This study aims to assess the efficacy of propolis oil extract in promoting wound healing in rabbits. 
Methods: This experimental study was conducted on twelve adult male New Zealand rabbits (1.5 to 2 kg) in two groups based on healing intervals of three and seven days. Two 8 mm incisions were created on the back region of each rabbit using a sterile biopsy punch. In the control group, wounds were allowed to heal naturally, while the experimental group received daily applications of local propolis oil (10 µl). Animals were sacrificed at each healing time point, and wound contraction was assessed visually. Histological and histomorphometric analyses were conducted to evaluate inflammatory cell counts, blood vessel counts, and epithelial thickness. 
Findings: On day three, a significant difference in wound contraction between groups was noted (11.7±1.3 vs 14.2±1.7) (p=0.01). On day 7, this difference persisted (41.5±8.4 vs 54.2±8.1) (p=0.02). No significant differences in inflammatory cell counts were observed on day three. However, by day seven, a significant difference was noted (p=0.001). Epithelial thickness showed significant differences on day three (5.3±0.8 vs 7.2±1.08) (p=0.008) and day seven (10.8±1.7 vs 17.3±2.6) (p=0.001). Blood vessel count differed significantly between groups on both days (p=0.01). 
Conclusion: The findings indicate that locally applied propolis oil can effectively enhance cutaneous wound healing through its anti-inflammatory properties and promotion of proliferative cell activity.