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An Evaluation of the Effect of Lettuce Seed Oil on Buccal Mucosa Healing
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N Bahjet Kamil *    |
| 1.Department of Oral Diagnosis, College of Dentistry, University of Baghdad, Baghdad, Iraq. , nawar.bahjat@codental.uobaghdad.edu.iq |
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Abstract: (18 Views) |
Background and Objective: The oral mucosa acts as a barrier against pathogens, and in cases of trauma, we are looking for a way to accelerate their recovery. Since lettuce seed oil has antibacterial, anti-inflammatory, and antioxidant effects, this study was conducted to evaluate the effect of lettuce seed oil on the repair of buccal mucosa in rats.
Methods: In this experimental study, 24 adult male rats were used. Incisional wound with a length of about 1 cm was created by a sharp knife on the left and right side of the buccal mucosa in each rat. The total number of wounds was 48 and the rats were randomly divided into two groups: Control group included 12 rats and 24 wounds that were divided into 3 healing periods (1, 3 and 7 days) left without treatment. The experimental group included 12 rats and 24 wounds which were treated with 5µL of lettuce oil applied by micropipette and they were divided into 3 healing periods (1, 3 and 7 days). The specimens were prepared and stained with hematoxylin and eosin as well as Masson's trichrome and they were evaluated.
Findings: Compared with the control group, the rate of epithelial regeneration increased in the experimental group. After 24 hours, there was a significant difference between the control and experimental groups in inflammatory cells (9.82±0.96 vs. 28.39±5.01) (p=0.000). After 72 hours, the experimental groups recorded higher mean epithelial thickness (9.58±1.23), blood vessels (8.51±0.75) and collagen fiber density (38.62±4.01) compared to the control group (4.67±0.08, 3.47±0.79 and 15.85±2.57, respectively). There was a significant difference in inflammatory cells, epithelial thickness and collagen fiber intensity (p=0.000). After the seventh day, there was a significant difference in all parameters between the control and experimental groups except blood vessels (p=0.000).
Conclusion: Lettuce seed oil has an effective role in acceleration of buccal mucosa healing in rats. |
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| Keywords: Lettuce Seed Oil, Masson's Trichrome Staining, Healing. |
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Type of Study: Research |
Subject:
Restorative Dentistry
Received: 2024/07/7 | Accepted: 2024/10/12
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