Background and Objective: Silver oxide nanoparticles are highly active against a wide range of microbes and parasites and also have lower toxicity for human cells than other biocidal heavy metals. The aim of the study was to determine the cytotoxicity effects of silver oxide nanoparticles on gingival fibroblasts.
Methods: This experimental study was carried out in laboratory conditions. 15 samples in 4 groups with concentrations of 10, 20, 30 and 40 µg/ml were examined at 2, 24, 48 and 72 hours. Dynamic Light Scattering (DLS) and Scanning Electron Microscope (SEM) were used to determine the characteristics of silver oxide nanoparticles, and MTT method was used to determine the toxicity of silver oxide nanoparticles.
Findings: Based on the result, in 24 hours of proximity to silver oxide nanoparticles, the average difference between the control group (0.19±0.01) with concentration of 40 (0.17±0.01) and the concentration of 10 0.19±0.01) with concentration of 40 (0.17±0.01) was statistically significant (p<0.001). At 48 hours of proximity, the difference in the mean OD between the control with concentrations of 40 (0.25±0.01) and 30 (0.25±0.01) was statistically significant (p<0.001). Also, at 72 hours, the difference in mean OD between the control with concentrations of 40 (0.30±0.01) and 30 (0.31±0.01) was statistically significant (p<0.001). Also, in all four times (24, 2, 48, 72 hours), the highest average OD (Optic Density) was related to concentration of 10 and the lowest was related to concentration of 40.
Conclusion: The highest level of viability is related to the concentration of 10 μg/ml in 2 hours, so it can be said that this concentration of silver oxide nanoparticles has acceptable biocompatibility. The lowest level of viability is related to the concentration of 40 μg/ml in 72 hours. |
