|
Synthesis and Toxicity Study of Silver Oxide Nanoparticles on Human Gingival Fibroblasts
|
A Mozaffari    , M Sharifyrad * , M Ranjbaran , M Radpour |
| 2.Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, I.R.Iran. , M.rad4294@gmail.com |
|
|
Abstract: (662 Views) |
Background and Objective: Silver oxide nanoparticles have very high activity against a wide range of microbes and parasites and also have lower toxicity to human cells compared to other biocides and heavy metals. The present study was conducted to determine the cytotoxic effects of silver oxide nanoparticles on gingival fibroblasts.
Methods: This experimental in vitro study was conducted on 15 samples in 4 groups at concentrations of 10, 20, 30 and 40 µg/ml within 2, 24, 48 and 72 hours. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) were used to determine the properties of silver oxide nanoparticles, and the MTT technique was used to determine the toxicity of silver oxide nanoparticles.
Findings: According to the results, after 2 hours of proximity to silver oxide nanoparticles, the mean difference was statistically significant between the control group (0.19±0.01) and the 40 µg/ml group (0.17±0.01), and also between the 10 µg/ml group (0.19±0.01) and the 40 µg/ml group (0.17±0.01) (p<0.001). After 48 hours of proximity, the mean difference in OD between the control group and concentrations of 40 µg/ml (0.25±0.01) and 30 µg/ml (0.25±0.01) was statistically significant (p<0.001). After 72 hours, the mean difference in OD between the control group and concentrations of 40 µg/ml (0.30±0.01) and 30 µg/ml (0.31±0.01) was statistically significant (p<0.001).
Conclusion: The results of the study showed that the highest viability was related to the concentration of 10 μg/ml and after 2 hours. Therefore, this concentration of silver oxide nanoparticles has acceptable biocompatibility. |
|
| Keywords: Silver Oxide Nanoparticles, Gingival Fibroblasts, Cytotoxicity. |
|
|
Full-Text [PDF 379 kb]
(121 Downloads)
|
Type of Study: Experimental |
Subject:
Dentistry (Oral Diseases)
Received: 2023/10/10 | Accepted: 2024/03/2 | Published: 2025/04/12
|
|
|
|
|
|
|
| Send email to the article author |
|
|
