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Investigation of Association between Two Polymorphisms Rs2235749 and Rs910080 in PDYN Gene and Susceptibility to Addiction
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A Hamta *    , P Ghasemian Safaei , R Bayatian |
| 1.Department of Biology, Faculty of Sciences, Arak University, Arak, I.R.Iran. , a-hamta@araku.ac.ir |
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Abstract: (47 Views) |
Background and Objective: Previous studies show that genetic factors, including polymorphisms, cause individual differences in the risk of addiction. One of the significant genes whose polymorphisms are related to the risk of addiction is PDYN gene. The aim of this study is to investigate the association between the two polymorphisms rs2235749 and rs910080 in PDYN gene and susceptibility to addiction.
Methods: In the current study, which is a case-control study, the blood samples of 80 drug addicts with a history of heroin or methamphetamine use and 80 healthy people with no history of use were examined from people living in Central Province. The genotype of individuals in the two studied groups was determined using PCR-RFLP technique and agarose gel electrophoresis. The statistical analysis of this study was done by using the Chi-square statistical test and considering P˂0.05 as significant values, by SPSS version 26 software.
Findings: Statistically, a significant difference was observed between the addicted and control groups for rs910080 polymorphism genotypes (p=0.000). In this study, the frequency of TT, CC, and CT genotypes was 43.75%, 15.25%, and 41.25% respectively in the control group and 25.46%, 46.25%, and 28.75% respectively in the addict group. In contrast, from the Statistical point of view, there was no significant difference between the addicted and control groups for the genotypes of the rs2235749 polymorphism (p=0.603). And the frequency of TT, CC, TC genotypes in the addict group was 23.75%, 40%, 36.25% and in the control group, 22.5%, 33.75%, and 43.75% respectively.
Conclusion: According to the results of this study, CC genotype of rs910080 PDYN gene polymorphism has a significant relationship with addiction and increases the risk of addiction, while people with CT and TT genotypes have a lower susceptibility to addiction. |
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| Keywords: Heroin, Polymorphism, Methamphetamine, PDYN. |
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Type of Study: Case-Control |
Subject:
Genetics, Cell and Molecular Biology
Received: 2024/08/10 | Accepted: 2024/12/2
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