[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 19, Issue 7 (7-2017) ::
JBUMS 2017, 19(7): 50-56 Back to browse issues page
Effect of Aqueous Extract of Artemisia absinthium L. on Sex Hormones, Inflammatory Cytokines and Oxidative Stress Indices of Ovarian Tissue in Polycystic Ovary Syndrome Rat Model
S.D Sadoughi *, R. Rahbarian, N. Jahani, S.A. Shazdeh, S. Hossein Zadeh Saljoughi, M. Daee
Young and Elite Research Club, Mashhad Unit, Islamic Azad University, Mashhad, I.R.Iran , damoon.sadughi@gmail.com
Abstract:   (4058 Views)

BACKGROUND AND OBJECTIVE: Hormonal disorders along with oxidative stress and inflammation in ovarian tissue lead to anovulation in polycystic ovary syndrome (PCOS) patients. Considering antioxidant effect of Artemisia absinthium, purpose of this study is to determine the effect of aqueous extract of Artemisia absinthium on serum levels of sex hormones, inflammatory cytokines and oxidative stress indices of ovarian tissue in polycystic ovary syndrome rat model.
METHODS: In this study, 32 female Wistar rats were divided into 4 equal groups: groups of control, PCOS control and two groups with PCOS which are under treatment through aqueous extract of Artemisia absinthium (100 and 200 mg/kg). Polycystic ovarian syndrome was induced by single intramuscular injection of estradiol valerate (4mg/kg). Aqueous extract of Artemisia absinthium was intraperitoneal injection to PCOS treated groups, for 24 days. At the end of treatment period, serum levels of LH, FSH, Estradiol, Testosterone, TNF-α, IL-1β and IL-6 and levels of SOD, CAT, GPX enzymes and level of MDA in ovarian tissue were measured through ELISA method.
FINDINGS: Compared to PCOS control group (LH: 14.30±2.52, FSH: 2.35±0.28, Estradiol: 17.61±2.44, Testosterone: 10.29±1.56, TNF-α: 178.65±4.35, IL-1β: 121.52±5.17, IL-6: 162.28±5.83, SOD: 20.51±1.84, CAT: 64.42±3.70, GPX: 35.15±2.88, MDA: 87.32±3.40), MDA tissue level (55.46±4.73), serum levels of LH (8.26±1.36), Estradiol (7.76±1.55), Testosterone (6.40±1.04) and TNF-α (115.35±5.83), IL-1β (70.25±5.74) and IL-6 (89.15±4.52) cytokines in group under treatment with 200 mg/kg aqueous extract of Artemisia absinthium significantly decreased (p=0.008) and serum level of FSH (7.52±1.21) and levels of SOD (71.58±5.19), CAT (128.30±5.11) and GPX (88.21±5.51) antioxidant enzymes of ovarian tissue significantly increased (p=0.005).
CONCLUSION: Aqueous extract of Artemisia absinthium by decreasing the serum levels of inflammatory cytokines and increasing the activity of the ovary tissue antioxidant enzymes has a favorable effect on the improvement of hormonal parameters in rats with polycystic ovary syndrome

Keywords: Polycystic Ovarian Syndrome, Artemisia absinthium, Oxidative stress, Cytokines, Rats
Full-Text [PDF 244 kb]   (1436 Downloads)    
Type of Study: Experimental | Subject: Phisiology
Received: 2017/02/21 | Accepted: 2017/07/12 | Published: 2017/08/15
Send email to the article author



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadoughi S, Rahbarian R, Jahani N, Shazdeh S, Hossein Zadeh Saljoughi S, Daee M. Effect of Aqueous Extract of Artemisia absinthium L. on Sex Hormones, Inflammatory Cytokines and Oxidative Stress Indices of Ovarian Tissue in Polycystic Ovary Syndrome Rat Model. JBUMS. 2017; 19 (7) :50-56
URL: http://jbums.org/article-1-6650-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 19, Issue 7 (7-2017) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

The Journal of Babol University of Medical Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Persian site map - English site map - Created in 0.04 seconds with 32 queries by YEKTAWEB 4343