An Investigation of the Effect of Hypoxia on Expression of 107-miR in Gastric Cancer Cell Lines MKN-45 and AGS
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N Ayremlou , H Mozdarani * , SJ Mowla , A Delavari |
, mozdarah@modares.ac.ir |
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Abstract: (7887 Views) |
BACKGROUND AND OBJECTIVE: Hypoxia in solid
tumors is the major cause of cancer treatment resistance. Thus, identifying the
appropriate indicators of hypoxia in tumors is of great importance for
appropriate tumor prognosis and choosing the best treatment methods. This study
aims to investigate the modulation of miR-107 expression, as a biomarker of
hypoxia and its association with gastric cancer cells.
METHODS: MKN45 and AGS gastric cancer cells were
purchased from Tehran Pasteur Institute and then, were cultured under normal
oxygen conditions (CO2 5% and O2 95%) and hypoxia (CO2
5% and N2 95%). Finally, miR-107 and HIF-1-α expressions were
evaluated every 24 to 48 hours.
FINDINGS: The results of the study showed that
through hypoxia induction, HIF-1-α expression in MKN-45 cell lines increased by
extending treatment duration (24 and 48 hours) 2.3 and 3.8 times, respectively,
(p=0.04 and p=0.002), and in AGS cell lines HIF-1-α expression increased 2.2
and 3.8 times (p=0.03 and p=0.005). In addition, miR-107 expression in MKN-45
increased during this time by 2.2 and 3.1 times (p=0.01 and p=0.0001), while in
AGS cell lines it was by 2.4 and 4 times (p=0.002 and p=0.0001).
CONCLUSION: It was demonstrated that hypoxia induction in gastric cancer
cells could modulate the expression of miR-107. |
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Keywords: Gastric cancer, HIF-1-alpha, Hypoxia, miR-107 |
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Full-Text [PDF 542 kb]
(4827 Downloads)
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Type of Study: Research |
Subject:
Genetics, Cell and Molecular Biology Received: 2015/02/27 | Accepted: 2015/08/25 | Published: 2015/09/20
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