Isolation and Differentiation of Dental Pulp Stem Cells into Odontoblastic Lineage
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Maryam Raoof * , Ali Mohammadi Kamal-abadi , Mohammad Mehdi Yaghoobi , Razieh Kooshki , Mehdi Abbasnejad , Ali Derakhshani |
Department of Endodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran. , maryam.raoof@gmail.com |
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Abstract: (9926 Views) |
BACKGROUND AND OBJECTIVE: Limited resources for adult
stem cells necessitate appropriate methods for isolation, proliferation and
differentiation of these cells. Human third molar is an easily available source
in this regard that usually remains unused.
The aim of this study was to investigate the isolation, culture and odontogenic
differentiation of human dental pulp stem cells. METHODS: In this experimental study, twenty human third molars were
used for stem cell isolation. The digested pulp tissue culture approach was
used to isolate stem cells. Some markers of stem cells were studied by RT-PCR.
Stem cells were then induced towards odontoblast cells by use of BMP-2 and
Dexamethasone. Expression of odontoblastic markers was investigated in induced
cells by RT-PCR and immunocytochemistry methods. FINDINGS: The human dental pulp stem cells were
isolated with high efficiency. RT-PCR
analysis showed that these cells express some markers of embryonic stem cells.
However, the induced cells did not expressed odontoblastic markers such as
DSPP, DMP-1, BMP7 and Osteopontin.
Also the induced cells did not produce DSPP and DMP-1 proteins. CONCLUSION: Despite the successful isolation of stem cells, the induced
cells failed to differentiate into odontoblasts. This may be due to the
differentiation method as well as the passage during induction or monolayer
culture.
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Keywords: KEY WORDS: Dental Pulp Stem Cells, Odontoblasts, BMP-2, Dexamethasone. |
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Full-Text [PDF 692 kb]
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Type of Study: Experimental |
Subject:
Dentistry (Endo) Received: 2014/07/21 | Accepted: 2014/10/26 | Published: 2014/11/19
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