|
Analysis of the Association between Genetic Polymorphism in Sodium Channel Nav1.8 Coding Gene and the Success of Inferior AlveolarNerve Block (IANB) in Mandibular Molar Teeth with Symptomatic Irreversible Pulpitis
|
A Salem Milani    , K Jamalvand * , S Mansouri Derakhshan , M Alivand , L Rostamizadeh , V Zand , A Nouroloyouni , T Barati , Z Mirzaei |
| 1.Department of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, I.R.Iran. , khatere.jamalvand13@gmail.com |
|
|
Abstract: (30 Views) |
Background and Objective: Inferior alveolar nerve block (IANB) technique is the most common technique used for mandibular anesthesia. Considering several factors that can cause the failure of this technique, the aim of the present study was to investigate the possible relationship between common genetic polymorphisms (rs6801957 and rs6795970) in the SCN10A gene and the success rate of IANB in mandibular molar teeth with symptomatic irreversible pulpitis.
Methods: In this study, 194 patients (107 women and 87 men) who referred for the root treatment of the first or second mandibular molars with sympthomatic irreversible pulpitis were included in the study. Saliva samples from the patients before the IANB administration were collected. Then, IANB anesthesia with 2% lidocaine with epinephrine 1/80,000 was administered. Ten minutes later, in case of lip numbness and lack of response to the cold and electric pulp tests, root canal treatment was started. The patients were told to raise their hand in case of any pain and rate their pain using the VAS technique. After DNA extraction and detection of rs6801957 and rs6795970 polymorphisms using the ARMS-PCR Tetra technique, the anesthesia success and failure rate was evaluated. The data were statistically analysed with Chi-square, Independent samples T-test and logistic regression test at error level of 0.05.
Findings: The anesthesia success rate in different alleles and genotypes of rs6801957 polymorphism does not show any significant difference. But the success rate in different genotypes of rs6795970 polymorphism has a significant difference p=0.015). Subjects with AG genotype experienced more anesthesia failure than AA and in subjects with GG less anesthetic failure occurred. However, the anesthesia success rate in different alleles of rs6795970 polymorphism did not show any significant difference. The results of regression analysis also showed that the frequency of genotypes of rs6801957 and rs6795970 polymorphisms in men and women have significant differences between anesthesia failure and success groups (p=0.030). So that the chance of anesthesia failure in men is 1.839 times more than women. Also, the results of the Chi-Square analysis was conducted to investigate the anesthesia success rate in different genotypes and alleles of rs6801957 and rs6795970 polymorphisms in different genders.The results showed that frequency of genotypes of both polymorphisms in men and women have a significant difference between the anesthesia failure and success groups, and the frequency of the G allele in the rs6801957 polymorphism and the A allele in the rs6795970 polymorphism showed a significant difference.
Conclusion: SNPs in SCN10A gene affect the success rate of pulp anesthesia following IANB.
|
|
| Keywords: Irreversible Pulpitis, Polymorphism, SCN10AT. |
|
|
|
Type of Study: Research |
Subject:
Dentistry (Endo)
Received: 2024/11/19 | Accepted: 2025/02/4
|
|
|
|
|
|
|
| Send email to the article author |
|
|
