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The Effect of Access Cavity Design and Three Single-File Rotary Systems on the Reduction of Enterococcus Faecalis Count in the Root Canal System
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M Emamgholipour    , S Mirzaee Rad * , A Pournajaf , SA Seyedmajidi |
| 1.Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran. , sinamirzaeerad@yahoo.com |
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Abstract: (174 Views) |
Background and Objective: The main goal in root canal therapy is to minimize the microbial load as much as possible. Since Enterococcus faecalis multiplies and forms biofilms on the wall of root canal, the aim of this study was to investigate the effect of access cavity design and three single-file rotary systems on reduction of Enterococcus faecalis count in the root canal system.
Methods: This in vitro study evaluated 60 extracted sound single-rooted single-canal mandibular premolars with long oval root canal cross-section. The teeth were randomly assigned to two groups (n=30) for preparation of traditional endodontic cavity (TEC) and conservative endodontic cavity (CEC). The root canals were prepared, and inoculated with E. faecalis (ATCC29212) for 4 weeks. Each group was randomly divided into three subgroups (n=10) for instrumentation with Reciproc, OneShape, and Only One File rotary systems. Bacterial samples were taken before (S1) and after (S2) instrumentation, using sterile paper points. Reduction in microbial load was calculated and reported in colony forming units (CFUs/mL).
Findings: The reduction in microbial load was significantly greater in TEC design (99.85±0.04) compared to CEC design (99.76±0.06) (p<0.05). The reduction in microbial load was not significantly different between the three files in TEC or CEC design.
Conclusion: Based on the results of this study, TEC design resulted in significantly greater reduction in E. faecalis count in the root canal system, but the three single-file rotary systems had no significant difference in this respect in any cavity design. |
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| Keywords: Enterococcus Faecalis, Root Canal Therapy, Microbial Load |
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Full-Text [PDF 360 kb]
(36 Downloads)
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Type of Study: Applicable |
Subject:
Dentistry (Endo)
Received: 2024/06/17 | Accepted: 2024/09/17 | Published: 2025/12/13
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