|
The Effect of Aerobic Exercise and Berberine Chloride on Mitochondrial Biogenesis Indices of Visceral Adipose Tissue of Diabetic Rats
|
S Asghari Vostakolaei    , S Farajtabar Behrestaq * |
| 1.Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, I.R.Iran. , farajtabarp@yahoo.com |
|
|
Abstract: (59 Views) |
Background and Objective: Oxidative stress contributes to insulin resistance, beta-cell dysfunction, and hyperglycemia-induced cellular damage leading to diabetes mellitus. The purpose of this study was to explain the effect of aerobic exercise and berberine chloride on the expression of UCP-1 and PPARγ genes in the visceral fat tissue of diabetic rats.
Methods: In the present study, the research method was experimental. To conduct this laboratory study, 32 adult male Wistar rats weighing about 240-280 were randomly divided into four groups. (n=8): diabetes (DM), diabetes-berberine (DMB), diabetes-aerobic exercise (DMT), diabetes-aerobic exercise-berberine (DMTB) were divided. Mice became diabetic by injecting 60 mg/kg of STZ. Rats whose blood sugar was higher than 300 mg/dl were included in the present study as diabetic animals. Then, the training groups performed an increasing aerobic exercise program (10-18 m/min, 10-40 minutes per day, five days per week) on a treadmill for six weeks. The supplement group also received berberine chloride (30 mg/kg/day) orally by gavage once a day.
Findings: Data analysis showed that there is a significant difference in the expression of UCP-1 (p=0.000) and PPARγ (p=0.002) genes among the groups. Further, the results showed that the expression of UCP-1 gene in diabetes-exercise group (2.96±1.85 and p=0.015), diabetes-berberine chloride (2.88±1.16 and p=0.02) and diabetes-exercise-berberine chloride group (4.7±0.97 and p=0.000) increased significantly compared to the group He had diabetes. Also, PPARγ gene expression in the diabetes-exercise-berberine chloride group (1.6±0.36 and p=0.002) had a significant increase compared to the diabetes group.
Conclusion: Based on the above findings, aerobic exercise and berberine chloride is one of the possible ways to prevent inflammation and damage caused by diabetes.
|
|
| Keywords: Aerobic Exercise, Berberine Chloride, Diabetes, UCP-1, Pparγ. |
|
|
|
Type of Study: Experimental |
Subject:
Sport Physiology
Received: 2023/10/12 | Accepted: 2024/01/13
|
|
|
|
|
|
|
| Send email to the article author |
|
|
