|
Evaluation of the Genistein Effects on Peroxiredoxin-4 and Oxidative Stress in A549 Lung Cancer Cell
|
R Farsiabi    , I Khodadadi , J Karimi , Gh Shafiee * |
| 1.Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, I.R.Iran. , g.r_shafiee@yahoo.com |
|
|
Abstract: (163 Views) |
Background and Objective: Genistein is a substance from the group of isoflavones that has been reported to have anti-cancer properties in various cancers. This study was designed to investigate the effect of genistein on oxidative stress markers in lung cancer cell line A549.
Methods: This is an experimental study that the 0, 20, 40, 60, 80, 100 µM of genistein concentrations were used to treat A549 cells and then cell survival was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide MTT assay after 24 and 48 hours. After selecting the IC50 dose Total antioxidant capacity (TAC) and total oxidant status (TOS) were studied using the manually assay, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity and peroxiredoxin-4 (PRXD4) tests were analyzed by the ELISA kits.
Findings: The IC50 dose of genistein for A549 cells was 40 μM in 24 h. The 40 μM dose of genistein was significantly differentiate for TAC in compared with the control (0.05±0.01, 0.07±0.01 mmol/mg respectively). There was a significant increase in TOS (0.03±0.004, 0.01±0.002 mmol/mg respectively) and OSI (0.57±0.08, 0.18±0.06 respectively) compared with the control group (p<0.05). Our results also showed that SOD (0.95±0.02, 2.56±0.19 UI/mg respectively), CAT (0.45±0.11, 1.23±0.18 UI/mg respectively) and PRXD4 (18.35±1.45, 42.83±3.75 pg/mg respectively) were significantly reduced in the treated groups compared to the control group (p<0.05). GPx was significantly reduced at a dose of 60 μM (85.28±4.36, 141.59±12.36 UI/mg respectively) compared to the control group (p<0.05).
Conclusion: Genistein reduces the survival and induces oxidative stress in A549 cancer cell line. |
|
| Keywords: Genistein, Oxidative Stress, A549, Lung Neoplasms. |
|
|
|
Type of Study: Research |
Subject:
Biochemical
Received: 2023/05/31 | Accepted: 2023/10/23
|
|
|
|
|
|
|
| Send email to the article author |
|
|
