:: Volume 19, Issue 1 (1-2017) ::
JBUMS 2017, 19(1): 36-42 Back to browse issues page
The Antibacterial Effects of Methanol Extract of Ammi majus on Staphylococcus aureus and Escherichia coli
P Sajadi Kabodi , D Bakhshi , AA Moghadamnia , AA Sefidgar
Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran , moghadamnia@yahoo.com
Abstract:   (1757 Views)

BACKGROUND AND OBJECTIVE: Diversity of plants and the increasing tendency to use them for therapies has increased the significance of screening herbal extracts. Several researches have reported the antibacterial effects of plants. Therefore, the present study aims to investigate the antibacterial effects of methanol extract of Ammi majus.

METHODS: After preparing methanol extract (0.5, 1 and 2%), the antibacterial effects of the plant were measured according to minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), disk diffusion method (determining the zone of inhibition) and well diffusion method on Staphylococcus aureus and Escherichia coli.

FINDINGS: In well diffusion method, the lowest concentration (0.5%) created a 9±0.000 mm inhibition zone diameter, while the highest concentration (2%) created a 12.3±0.57 mm inhibition zone diameter (p<0.001). The photometric tests revealed that the methanol extract of the plant (0.5 to 1%) with mean difference of 0.04 had antibacterial effects on Escherichia coli (p<0.001). However, as the concentration increased (2%), adverse non-inhibitory effects could be observed. The tests based on colony counting method demonstrated that Ammi majus extract 0.5, 1 and 2% have antibacterial effect on Staphylococcus aureus (p<0.001). However, only the 2% extract had inhibitory effects on Escherichia coli (p<0.01).

CONCLUSION: Results of the study demonstrated that methanol extract of Ammi majus has more antibacterial effect on Staphylococcus aureus compared with Escherichia coli.

Keywords: Extract, Ammi majus, Staphylococcus aureus, Escherichia coli
Full-Text [PDF 287 kb]   (554 Downloads)    
Type of Study: Research | Subject: pharmacology
Received: 2016/08/14 | Accepted: 2017/01/15 | Published: 2017/03/14

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Volume 19, Issue 1 (1-2017) Back to browse issues page