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Toxicity Effect of Assa-Foetida L Extract on C2C12 Cells
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J. Mohiti-Ardekani (PhD) *   , S. Abedini (MSc) |
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Abstract: (9692 Views) |
BACKGROUND AND OBJECTIVE: Assa-Foetida L is traditionally used in treatment of different diseases including asthma, roundworm, stomach reflex, flu and nervous disease. Although some studies have done on Assa-Foetida properties, but little studies have been done on its cellular and molecular effects. The aim of this study was to evaluate the amount of polyphenols in Assa-Foetida L and its toxicity effect on C2C12 as a cell model of glucose consumer.
METHODS: At first, hydroalcoholic extract of Assa-Foetida L was prepared. The polyphenols of Assa-Foetida was determined using gallic acid as a standard by Folin–Ciocalteu’s method. The toxicity of the herbal on C2C12 cells was evaluated using Tripan blue at concentration of 50-2500µg/µl for 3 hours.
FINDINGS: Hydroalcoholic extract of Assa-Foetida L contain polyphenol contents. Absorbance of Assa-Foetida L increases linearly with gallic acid absorbance (R2=0.9989 vs R2=9986). Different concentrations of Assa-Foetida L extract showed that its hydro-alcoholic extract up to 200 µg/µl concentration had no toxicity effect on C2C12 and the cells were stable in treated culture of Assa Foetida after 3 hours. There was no significant difference in viability rate of cells between treated and control groups.
CONCLUSION: The results of this study showed that Assa-Foetida L extract up to 200 µg/µl concentrations had no toxicity effect on these cells after 3 hours. |
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| Keywords: Assa-Foetida, C2C12 cells, Polyphenolic compounds |
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Full-Text [PDF 1907 kb]
(2308 Downloads)
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Type of Study: Research |
Subject:
Biochemical
Received: 2013/12/14 | Accepted: 2014/06/14 | Published: 2014/07/15
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