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The Relationship between microRNA-125b and Serum Amyloid a Protein (SAA) in the Diagnosis and Prognosis of Colorectal Cancer
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H Jaber Waheed *    , M Essam Abdalah , W Khalid Ahmed , S Abdulsattar Oudah Al-Qaysi |
| 1.Department of Pharmacology and Toxicology, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq. , dr.huda.jw@uomustansiriyah.edu.iq |
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Abstract: (529 Views) |
Background and Objective: Serum amyloid A (SAA) protein increases in case of infection or inflammation, and especially in the serum of cancer patients. Regulation of the genes encoding this protein by microRNAs in cancers has been reported in several studies. The aim of this study is to explore the cumulative effect and interaction between SA1 and miR-125b in serum as a potential diagnostic and prognostic marker for colorectal cancer.
Methods: In this case-control study, 55 patients with colorectal cancer were compared with 50 healthy individuals. SAA levels were measured using the ELISA technique and miR-125b expression was assessed by rtPCR. Alkaline phosphatase was measured using a spectrophotometer and serum ferritin levels were measured and compared by CLIA analyzer.
Findings: There was a significant difference in the mean value of SAA in patients when compared to control (27.43 12.9 μg/ml vs. 4.84±0.8 μg/ml, respectively) (p<0.0001). There was a significant change in the gene expression in microRNA-125b expression in patient group, which was 2.76-fold higher than that of control (p<0.05). There was a significant difference in serum ferritin levels in patients (53.2±11.4 ng/ml) when compared to control (13.64±5.5ng/ml). There was a significant difference in ALP level when compared between colon cancer patients (94.4±17 IU/L) and control (273.8±83 IU/L). SAA levels significantly increased in colorectal cancer patients in comparison to healthy subjects.
Conclusion: According to the present study, SAA and microRNA-125b are promising markers to diagnose the colorectal cancer.
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| Keywords: Colorectal Cancer, Serum Amyloid A (SAA), microRNA-125b. |
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Full-Text [PDF 343 kb]
(107 Downloads)
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Type of Study: Research |
Subject:
surgery
Received: 2024/12/13 | Accepted: 2025/01/21 | Published: 2025/05/6
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