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Design, Synthesis and Evaluation of a Rapid Prostate-Specific Antigen Detection Test Strip
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M Shahri    , E Ferdosi-Shahandashti * , Gh Kardar |
| 2.Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran. , elaheh.ferdosi@yahoo.com |
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Abstract: (80 Views) |
Background and Objective: Early detection of all cancers, including prostate cancer, greatly helps in their treatment. In addition to clinical examination of the prostate gland, prostate-specific antigen (PSA) is also used to diagnose prostate cancer. Methods such as ELISA, PCR, radioimmunoassay, etc. have been used to detect PSA, which, in addition to being expensive, require an experienced technician, complex equipment, and a lot of time. The purpose of this study is to try to design and produce a rapid test for PSA measurement domestically and help produce and reduce imports and preserve foreign exchange in the country.
Methods: In this experimental study, first, colloidal gold nanoparticles were covalently conjugated with monoclonal antibodies, then different components of the diagnostic test strip were examined for their type and pore size, and different parts of the test strip were assembled and the antibodies were loaded onto the substrate. Serum samples from healthy and diseased individuals with different serum dilutions ranging from 1 to 9 ng/mL for prostate-specific antigen were obtained from a diagnostic laboratory that had previously been measured with VIDAS. Finally, the sera were measured with the designed test strip. Finally, the test strip was examined for accuracy and precision.
Findings: In measuring different serum dilutions of prostate-specific antigen from 1 to 9 ng/mL, the test strip designed in this study showed similar results to the commercial kit, demonstrating the accuracy and sensitivity of the test strip designed in this study.
Conclusion: A rapid prostate cancer detection test strip based on PSA measurement was designed and prototyped. |
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| Keywords: Prostate Cancer, Gold Nanoparticles, Prostate-Specific Antigen, Lateral Flow Immunoassay Strip Test. |
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Type of Study: Applicable |
Subject:
Urology
Received: 2024/11/16 | Accepted: 2025/01/20
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