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:: Volume 19, Issue 12 (12-2017) ::
JBUMS 2017, 19(12): 63-68 Back to browse issues page
Comparison of the Effect of Artemisia Sieberi Essential Oil and Albendazole Drug on Protoscolices of Hydatid Cyst under in Vitro Conditions
MJ Hosseini , MR Yousefi , M Abouhosseini
Department of Parasitology, Babol Branch, Islamic Azad University, Babol, I.R.Iran , youssefi929@hotmail.com
Abstract:   (456 Views)

BACKGROUND AND OBJECTIVE: Hydatidosis is a major zoonotic disease, and the best treatment for this disease is surgery. So far, many chemical protoscolex killers have been used to prevent protoscolices leakage during surgery, but it is necessary to consider the adverse effects of chemicals and the use of medicinal herbs. Considering the antiparasitic properties of Artemisia sieberi, this study was conducted to evaluate and compare the effect of Artemisia sieberi essential oil and albendazole drug on protoscolices of hydatid cyst under in vitro conditions.
METHODS: In this laboratory study, essential oils were prepared from aerial parts of the plant. Gas chromatography–mass spectrometry was performed to determine its components. Protoscolices were extracted from the livers infected with hydatid cyst and were exposed to Artemisia sieberi (2.5, 5 and 10 μg/ml) for 10, 30, 60 and 120 minutes. The viability of protoscolices was measured by eosin staining. Albendazole was used as a standard drug.
RESULTS: 31.5% alpha-Thujone was identified as the main composition pf essential oil. The amount of essential oil protoscolices at the concentration of 2.5 μg / ml in 10, 30, 60 and 120 minutes was 51.8, 71.5, 82.8 and 99.3%, respectively. Albendazole showed lower toxic effect with similar dose at similar intervals, but both treatments showed a significant effect (p<0.05). The highest toxic effect of essential oil was observed at 10 μg/ml concentration 30 minutes after treatment. For albendazole, this effect was observed at a dose of 10 μg / ml after 120 minutes.
CONCLUSION: The results of the study showed that Artemisia sieberi essential oil has an acceptable scolicidal effect compared to albendazole and can be used as a scolicidal agent.

Keywords: Hydatid cyst, essential oil, Artemisia, Gas chromatography–mass spectrometry
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Type of Study: Research | Subject: A
Received: 2017/07/1 | Accepted: 2017/12/10 | Published: 2018/01/7
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Hosseini M, Yousefi M, Abouhosseini M. Comparison of the Effect of Artemisia Sieberi Essential Oil and Albendazole Drug on Protoscolices of Hydatid Cyst under in Vitro Conditions . JBUMS. 2017; 19 (12) :63-68
URL: http://jbums.org/article-1-6938-en.html


Volume 19, Issue 12 (12-2017) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

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