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:: Volume 18, Number 11 (11-2016) ::
JBUMS 2016, 18(11): 63-70 Back to browse issues page
A Study of Gene Expression and Activity of NorA Efflux Pump in Clinical Isolates of Ciprofloxacin Resistant Staphylococcus Aureus
A Mirzaie *, H Noorbazargan, H Rahmati, M Zandi
, Amir_mirzaie92@yahoo.com
Abstract:   (1441 Views)

BACKGROUND AND OBJECTIVE: The existence of efflux pumps such as norA is one the mechanisms of resistance to antibiotics like ciprofloxacin in staphylococcus aureus bacteria. NorA efflux pump and its gene expression and activity in ciprofloxacin resistant S. aureus strains are investigated in this study.

METHODS: In this experimental study, 250 clinical samples of blood, urine, wound and trachea were collected from patients hospitalized in various hospitals of Tehran. S. aureus isolates were detected and thereafter, antibiotic resistance profile, gene expression of norA efflux pump and its existence were investigated using PCR and Real-Time PCR and the activity of norA efflux pump was investigated using minimum inhibitory concentration (MIC).

FINDINGS: Of 250 clinical samples, 50 S. aureus isolates were detected and the antibiotic susceptibility test results revealed that 68% of samples (34 samples) were resistant to methicillin and 32% of samples (16 samples) were susceptible to methicillin and 24% of all samples (12 samples) were resistant to ciprofloxacin. Real-Time PCR test results revealed that each type of strain has a different expression of norA gene and more resistant strains have increased expression of norA gene. Moreover, all ciprofloxacin resistant strains had active efflux pump.

CONCLUSION: The results of the study demonstrated that there is a significant relationship between gene expression of norA efflux pump, its activity and resistance to ciprofloxacin in S. aureus strains

Keywords: Staphylococcus aureus, Resistance to ciprofloxacin, Efflux pump, NorA
Full-Text [PDF 366 kb]   (366 Downloads)    
Type of Study: Experimental | Subject: Mi
Received: 2016/06/25 | Accepted: 2016/11/14 | Published: 2016/12/27
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DOI: 10.22088/jbums.18.11.63


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Mirzaie A, Noorbazargan H, Rahmati H, Zandi M. A Study of Gene Expression and Activity of NorA Efflux Pump in Clinical Isolates of Ciprofloxacin Resistant Staphylococcus Aureus. JBUMS. 2016; 18 (11) :63-70
URL: http://jbums.org/article-1-6179-en.html
Volume 18, Number 11 (11-2016) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences
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