1 1561-4107 Journal of Babol University Of Medical Sciences 8004 Oncology A Case Report of Malignant Peripheral Nerve Sheath Tumor of the Breast Abdal Kh b Darvishi M c Hafezi Ahmadi M d b 1. Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam, I.R.Iran c 2. Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, I.R.Iran d 3. Department of Pathology, Ilam University of Medical Sciences, Ilam, I.R.Iran 1 12 2019 21 1 1 5 14 07 2018 23 01 2019 BACKGROUND AND OBJECTIVE: Breast sarcomas may be primary or secondary. Primary sarcoma of the breast is one of the extremely rare cases of breast malignancies whose possibility of occurrence is estimated to be 0.5% of all types of breast cancers and less than 5% of the all sarcomas .In this study, we present a woman with a malignant peripheral nerve sheath tumor in the brest. CASE REPORT: A 40-year old woman with a painless palpable mass in the right breast refered to imam khomeini hospital of one year ago. According to mammography and cytology and with the possibility of breast cancer patient undergoing surgery and the mass was removed. Histopathology feature and positive immunohistochemical markers such as S100 and vimentin confirmed malignant peripheral nerve sheath tumor of the breast (Triton type).Patient after mastectomy , radiation and chemotherapy regimen received and now, after 12 months no recurrence or metastasis has been seen. CONCLUSION: In breast cancer tumors, early diagnosis and appropriate therapeutic such as surgery , radiotherapy and chemotherapy are recommended to incrase overall survival and improve their quality of life.
7820 Heart Surgery An Evaluation of the Risk Factors of Coronary Artery Disease in Patients Undergoing Coronary Artery ByPass Graft Surgery in Babol Behzad C e Zakeri S f Vafaey HR g e 1.Clinical Research Development Unite of Rouhani Hospital, Babol University of Medical Sciences, Babol, I.R.Iran f 2.Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran g 1.Clinical Research Development Unite of Rouhani Hospital, Babol University of Medical Sciences, Babol, I.R.Iran 1 12 2019 21 1 6 10 06 05 2018 27 02 2019 BACKGROUND AND OBJECTIVE: Awareness of the prevalence of modifiable cardiovascular risk factors and attempts to correct and control it can be effective in reducing the chance of advanced cardiovascular disease that leads to complicated surgical procedures. Since the risk factors of advanced heart disease in northern Iran are unclear, this study was conducted to evaluate the risk factors of coronary artery disease in patients undergoing coronary artery bypass graft surgery in Babol, northern Iran. METHODS: This cross-sectional study was performed on 460 patients who underwent coronary artery bypass graft surgery from the beginning of 2011 to the end of 2015 in Ayatollah Rohani Hospital in Babol. The modifiable risk factors of coronary heart disease were evaluated according to the data in medical records of patients. FINDINGS: The prevalence of hypertension in the total samples was 56.74% (261 patients), the prevalence of hyperlipidemia was 32.16% (150 patients) and the prevalence of diabetes was 38.91% (179 patients). 31.09% (143 patients) had history of myocardial infarction in the past. 12.6% (58 patients) were smokers, all of whom were male. In terms of gender distribution, 53% (244 patients) were male and 47% (216 patients) were female. The mean age of the patients was 61.4±9.72 years and the mean weight of the patients was 68±12.57 kg. In terms of body mass index, the total mean was 26.3±4.25. CONCLUSION: According to the results of this study, hypertension, diabetes, and obesity are significantly common in the population of cardiac patients in Babol. The prevalence of risk factors in women is higher. Therefore, timely screening and precise monitoring of these isuues can be important in preventing long-term complications. 7649 Environmental Health Evaluation of the Capability of Carbon Dioxide Greenhouse Gas Absorption Using Nano Bio-Activated Carbon of Crataegus Sanguinea Core Farhadi P h Ahmadpour kacho H i Asgharnia HA j Masomi M k h 1. Department of Chemical Engineering, Faculty of Engineering, Islamic Azad University, Ayatollah Amoli Branch, Amol, I.R.Iran i 1. Department of Chemical Engineering, Faculty of Engineering, Islamic Azad University, Ayatollah Amoli Branch, Amol, I.R.Iran j 2. Environmental Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran k 1. Department of Chemical Engineering, Faculty of Engineering, Islamic Azad University, Ayatollah Amoli Branch, Amol, I.R.Iran 1 12 2019 21 1 11 18 03 03 2018 12 02 2019 BACKGROUND AND OBJECTIVE: Considerable increase in carbon dioxide gas in the Earth's atmosphere has caused several problems such as increasing the temperature of the earth, droughts and sudden changes in the climate. The purpose of this study was to evaluate the capability of carbon dioxide as greenhouse gas absorption using activated nanobiocarbon from the Crataegus Sanguinea core. METHODS: In this experimental study, the carbon dioxide gas penetration time in three subgroups with pressure (5,10,15 bars), flow (5,10,15 ml/min) and temperatures (20,30,60 °C) at concentrations of 0 to 1 gr / ml through a carbon dioxide gas capsule in a fixed bed column filled with 2 g of adsorbent synthesized by a carbon dioxide gas sensor. The synthesized nano-biocarbon adsorbent from the Crataegus Sanguinea Core was pyrolysed at a temperature of 300 ° C and activated at 600 °C. The research data were examined with pseudo -first-order and pseudo-second-order models. FINDINGS: Significant increase in gas infiltration time was observed at 15 bars pressure (86.71±0.09 min, p<0.001), temperature of 20 ºC (67.68±0.13 min, p≤0.001) and flow of 5 ml/min (75.78±0.11 min, p≤0.001) was more than 5 bars (37.14±0.17 min) and 10 bars (60.33±0.27 min) and 30 °C (54.36±0.03 min) and 60 ºC (45.34±0.31 min) and 10 ml/min (53.8±0.13 min) and 15 ml/min (45.09±0.09 min) respectively. Both models fit well with a coefficient of R2>0.98 on empirical data from absorption. CONCLUSION: The results of the study showed that increasing pressure and decreasing temperature and flow increase the time of carbon dioxide penetration onto synthesized adsorbent. 8380 Nursing The Relationship between Lifestyle and General Health of Elderly People Covered by HealthCare in Babol city Asadibrojeni Sh l Ilali ES m Taraghi Z n Mousavinasab N o Bijani A p l 1.Student Research Committee, Mazandaran University of Medical Sciences, Sari, I.R.Iran m 2.Health Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, I.R.Iran n 2.Health Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, I.R.Iran o 2.Health Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, I.R.Iran p 3-Social determinants of health research center, Health research Institute, Babol University Of Medical Sciences, Babol 1 12 2019 21 1 19 26 16 12 2018 26 03 2019 BACKGROUND AND OBJECTIVE: The better understanding of lifestyle and elderly health can be helpful in designing and prioritizing interventions for chronic diseases. The overall aim of this study was to determine the relationship between lifestyle and general health in elders. METHODS: In this cross-sectional study, 500 elders over the age of 60 who had no cognitive problems were selected from the health center in Babol city; according to the systematic cluster random sampling method. Sampling was done from April to July 2018. The research tools were the demographic questionnaire, the Iranian elderly lifestyle questionnaire and the general health questionnaire (GHQ) that were completed by interviewing. FINDINGS: The results showed that the Mean±SD age of the participants was 68.36 ± 6.5 years and 51.4% of the elderly had a favorable lifestyle and 66.4% had a desirable general health. The results showed that lifestyle and its domains had statistically significant reverse relation (p<0.001, R= -0.503) with general health and its subscales, except nutrition (p=0.06). The results of multivariable logistic regression analysis showed that income, location and lifestyle variables had a significant effect on general health. CONCLUSION: The findings of this study showed that half of the elderly who participated in this study were at a desirable lifestyle status and general health. Lifestyle, location and income were effective variables the general health of the elderly. It seems that along with educational-counseling interventions to improve lifestyle and raise the health of the elderly, the need for financial support also should be considered.   8143 Biochemical The Effect of Silymarin on the Expression of Urotensin–II and Urotensin–II Receptor Genes in the Liver Tissue of Type 2 Diabetic Rats Kheiripour N Karimi J Tayebinia H Khodaddi I Goudarzi MT 1. Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, I.R.Iran 2. Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, I.R.Iran 2. Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, I.R.Iran 2. Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, I.R.Iran 2. Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, I.R.Iran 1 12 2019 21 1 27 33 06 09 2018 08 01 2019 BACKGROUND AND OBJECTIVE: Studies have shown that the increase in urotensin – II is associated with diabetes disorders. Considering that using herbal medicines for the treatment of diseases leads to fewer complications compared to most chemical drugs, the present study was conducted to investigate the effect of silymarin on glucose, and insulin levels and the expression of urotensin – II (U–II) and urotensin – II receptor (U–II R) genes in the liver tissue of type 2 diabetic male rats. METHODS: In this experimental study, 36 male albino Wistar rats were randomly divided into 6 groups (n=6): 1. Control group; 2 and 3. Control groups treated with 60 and 120 mg / kg / day silymarin; 4. Type 2 diabetic group which received an intraperitoneal (i.p.) injection of 60 mg / kg streptozotocin and 120 mg / kg nicotinamide; 5 and 6. Diabetic rats treated with 60 and 120 mg/kg/day silymarin. After 60 days of treatment, serum and liver tissue samples were collected. Glucose, insulin, HOMA-IR index and liver enzymes were evaluated by spectrophotometry and ELISA methods, while gene expression in liver tissue was analyzed by Real-time PCR method. FINDINGS: Insulin levels increased significantly in diabetic groups treated with silymarin (60 and 120 mg/kg) (9.6±1.11 and 9.8±0.96, respectively) in comparison with the diabetic control group (7.10±1.06) (p<0.05). Moreover, glucose level, HOMA-IR, liver enzymes, U–II and U – II R expression in diabetic group treated with silymarin significantly decreased compared to diabetic control group (p<0.05).   CONCLUSION: The results of this study showed that administration of silymarin improves liver function in diabetic rats. 7816 Physiotherapy The Correlation between Craniovertebral Angle and Scapular Dyskinesis in Adults Khosravi F Rahnama L Karimi N Amiri M Department of Physiotherapy, University of Social Welfare and Rehabilitation Sciences, Tehran, I.R.Iran Department of Physiotherapy, University of Social Welfare and Rehabilitation Sciences, Tehran, I.R.Iran Department of Physiotherapy, University of Social Welfare and Rehabilitation Sciences, Tehran, I.R.Iran Department of Physiotherapy, University of Social Welfare and Rehabilitation Sciences, Tehran, I.R.Iran 1 12 2019 21 1 34 38 02 05 2018 27 01 2019 BACKGROUND AND OBJECTIVE: Faulty neck posture causes many musculoskeletal disorders in the neck and shoulders. Considering the shared muscle attachments of the neck and the scapula, identifying the correlation between neck posture and scapular position can be effective in preventing shoulder pain. Therefore, the present study was conducted to determine the relationship between neck posture and static and dynamic scapular position. METHODS: In this cross-sectional study, 38 female students from the University of Social Welfare and Rehabilitation Sciences without history of dysfunction in the shoulder and neck were selected and examined through nonprobability convenience sampling. Neck posture was examined by measuring the craniovertebral angle using diagnostic imaging techniques. The scapula – spine distance was measured using the ruler and the scapular winging was measured in hanging position of hand and during the flexion and scaption, and then the relationship between them and the craniovertebral angle was investigated.  FINDINGS: Subjects with an average age of 24.71±3.02 participated in the study. There was a significant correlation between craniovertebral angle (51.09±5.73) and the scapular winging in flexion (r=0.38, p=0.01) and scaption (r=0.44, p=0.005). There was no significant relationship between the craniovertebral angle and the distance between the fourth thoracic vertebra (T4) and the inferior angle of the scapula (6.29±0.96) and the distance between inferior angle and the corresponding vertebral levels (7.80±0.93). CONCLUSION: The results of this study showed that neck posture is correlated with scapular dyskinesis in a dynamic scapular position. 8033 Infectious Diseases The Prevalence of Nosocomial Infections in Iranian Hospitals Mohammadi M Vaisi Raiegan AA Jalali R Ghobadi A Salari N Barati H 1. Department of Nursing, Faculty of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, I.R.Iran 1. Department of Nursing, Faculty of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, I.R.Iran 1. Department of Nursing, Faculty of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, I.R.Iran 1. Department of Nursing, Faculty of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, I.R.Iran 1. Department of Nursing, Faculty of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, I.R.Iran 2. Department of Public Health, Faculty of Health, Sabzavar University of Medical Sciences, Sabzavar, I.R.Iran 1 12 2019 21 1 39 45 22 07 2018 23 01 2019 BACKGROUND AND OBJECTIVE: Nosocomial infections are one of the major health problems. As the length of stay in the hospital increases, the risk of mortality and morbidity increases, which ultimately increases the cost of treatment. Therefore, the present study was performed as a systematic review and meta-analysis to evaluate the prevalence of nosocomial infections in Iran. METHODS: This systematic review and meta-analysis was performed in the range of the years 2001 – 2017. Articles related to the topic were assessed using Persian keywords “nosocomial infections”, “hospital”, and “Iran”, and their English equivalent in descriptive and cross sectional studies by searching online databases of SID & Magiran, PubMed and Scopus, ScienceDirect and Google Scholar. Analytical and interventional studies were excluded from the study list. FINDINGS: 578 articles had the preliminary inclusion criteria, and with the removal of 568 unrelated or low quality articles during secondary analyses, 10 articles were finally included in the process of meta-analysis. The overall prevalence of nosocomial infections in Iranian hospitals was 4.6% (CI-95%: 2.6 – 8.1). The highest prevalence of nosocomial infections was in Sanandaj with 15.6% (CI-95%: 10.22–82.1) and the lowest prevalence was in Urmia with 0.4% (CI-95%: 0.1–1.01).  CONCLUSION: The results of the study showed that nosocomial infection has a low prevalence in Iran, but more attention and control over nosocomial infections in Iranian hospitals is necessary to reach standard levels.    7866 Microbiology Molecular Identification and Detection of Streptococcus Pneumoniae Serotypes Isolated from Selected Hospitals in Tehran Using Multiplex PCR Method RafieiTabatabaei S Fallah F Afshar D Nazari Alam A 1. Pediatric Infections Research Center, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 2. Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 3. Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, I.R.Iran 4. Department of Microbiology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I.R.Iran 1 12 2019 21 1 46 52 20 05 2018 27 01 2019 BACKGROUND AND OBJECTIVE: Streptococcus pneumoniae is one of the major pathogens responsible for invasive diseases such as pneumonia and meningitis. Epidemiological studies of these microorganisms are necessary to evaluate the effect of pneumococcal vaccine in any community. Therefore, the present study was conducted for molecular identification and detection of Streptococcus pneumoniae serotypes isolated from selected hospitals in Tehran using multiplex PCR method. METHODS: This cross-sectional study was performed on 32 isolates of Streptococcus pneumoniae from clinical specimens of patients admitted to different hospitals in Tehran. The isolates were identified by phenotypic tests and PCR method. Multiplex PCR was used to determine the serotype. FINDINGS: The number and percentage of Streptococcus pneumoniae isolates isolated from cerebrospinal fluid, sputum, blood, bronchoalveolar lavage, eyes, and nasal discharge were 12 (37.6%), 7 (21.8%), 6 (18.7%), 3 (9.5%), 2 (6.2%), and 2 (6.2%) isolates, respectively. In the present study, the identified serotypes were the serotypes 1, 4, 6A/B, 7F, 9V, 11A, 14, 15A, 19A, 19F, and 23F.  CONCLUSION: Based on the results of this study, more than 50% of the serotypes were not among the serotypes present in the vaccines that are commonly used in the community.   8023 Descriptive Sciences Effect of Different Doses of Curcumin on Sperm Parameters and Oxidative Stress in Testis of D-Galactose Induced Aging Mice Model Yousefi M Mohammadi Sh Jalali M Beheshti F 1. Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I.R.Iran 2. Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, I.R.Iran 1. Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I.R.Iran 3. Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I.R.Iran 1 12 2019 21 1 53 60 21 07 2018 23 01 2019 BACKGROUND AND OBJECTIVE: Aging is accompanied with low concentration of testosterone hormone and apoptosis induction in the testis. The aim of this research was to investigate the effects of different doses of curcumin as the active ingredient of Curcuma Longa Turmeric, on sperm quality and oxidative stress in mice D-galactose-induced aging model. METHODS: 48 Balb/c mice (n=8) were randomly assigned to 6 groups: control, Sham, D-galactose and Curcumin 1 to 3 groups. 300 mg/kg of D-galactose was injected to D-galactose group. Curcumin 1 to 3 were injected D-galactose + 25, 50 and 100 mg/kg curcumin intraperitoneally. Then, the oxidative stress based on biochemical parameters and sperm analysis according to WHO guideline were evaluated on day 42 of the experiment. FINDINGS: Mean sperm count in control group was (4.17±0.84) while it reduced after reception of 300 mg/kg D-galactose (3.06±0.86). There was a significant increase in the sperm parameters in Curcumin group compared to the D-galactose group (p<0.05). A significant increase was observed in the level of thiol and superoxide dismutase enzyme in curcumin group 3, compared to the D-galactose group (p≤0.001). Significant decreases in catalase and malondialdehyde enzymes were observed in the D-galactose group, compared to the curcumin 1, curcumin 2 and curcumin 3 groups (p<0.05). CONCLUSION: Administration of curcumin for 2 weeks improved sperm parameters and decreased oxidative stress in testis of mice D-galactose-induced aging model.   8126 Psychology The Effectiveness of Cognitive – Behavioral Group Therapy On Pain Perception And Pain Severity Among Patients With Chronic Neuropathic Pain Dehestani M Mirzaian B Saadat P 1. Department of Clinical Psychology, Sari Branch, Islamic Azad University, Sari, I.R.Iran 1. Department of Clinical Psychology, Sari Branch, Islamic Azad University, Sari, I.R.Iran 2. Mobility Impairment Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 1 12 2019 21 1 61 66 01 09 2018 13 04 2019 BACKGROUND AND OBJECTIVE: The experience of pain consists of two sensory and emotional dimensions. The sensory dimension of pain indicates pain severity and the emotional dimension indicates pain perception. Since cognitive–behavioral therapy is an evidence-based treatment and emphasizes on the correction of dysfunctional thought processes and changing the maladaptive behaviors, this study was designed to investigate the effectiveness of cognitive – behavioral group therapy on pain perception and pain severity among patients with chronic neuropathic pain. METHODS: This quasi-randomized trial was performed among 30 patients with chronic neuropathy referring to Rouhani Hospital in Babol in two groups of case and control (15 patients in each group). Cognitive – behavioral group therapy was performed in ten 120-minute sessions, held once a week for the experimental group. Participants were examined before the experiment and 45 days after the sessions, and the perception of pain was evaluated by the components of belief in pain permanence, self-blame, belief in pain constancy, mysteriousness of pain, and pain severity. The attainable score in The Pain Beliefs and Perceptions Inventory was 30 to -30, and in The West Haven-Yale Multidimensional Pain Inventory was 0 to 6.    FINDINGS: The results showed that there was a statistically significant difference between the two groups in the total score of pain perception (-8.87±7.40 vs. 1.6±93.30) (p=0.001), and the subscales of belief in pain permanence (-4.13±1.76 vs. 0.93±3.30) (p=0.04), belief in pain constancy (0.80±3.16 vs. -2.13±3.50) (p=0.04), mysteriousness of pain (-2.73±3.67 vs. 1.33±3.95) (p=0.003), and pain severity (2.19±1.28 vs. 3.64±1.27) (p=0.003). CONCLUSION: The present study showed that cognitive–behavioral group therapy could be an effective intervention for reducing the pain perception and pain severity in patients with chronic neuropathic pain. 7829 The Effectiveness of Religious-Spiritual Psychotherapy on the Quality of Life of Women with Breast Cancer Nasiri F Keshavarz Z Davazdahemami MH Karimkhani Zandi S Nasiri M 1. Department of Midwifery and Reproductive Health, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 1. Department of Midwifery and Reproductive Health, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 2. Department of Clinical Psychology, School of Medical, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 3. Department of Radiotherapy and Oncology, Kamkar Hospital, Qom University of Medical Sciences, Qom, I.R.Iran 4. Department of Statistics, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 1 12 2019 21 1 67 73 10 05 2018 23 01 2019 BACKGROUND AND OBJECTIVE: Breast cancer is one of the most common cancers around the world that leads to a decreased quality of life. Since one of the supportive measures is the use of spiritual teachings, the present study was conducted to investigate the effect of religious care on the quality of life of women with breast cancer. METHODS: This quasi-experimental study was conducted in 2017 among women with breast cancer who referred to Qom health centers at least two months after their diagnosis. Seventy subjects were selected through convenience sampling and were randomly assigned to intervention and control groups. For the intervention group, 6 sessions of religious – spiritual psychotherapy were held with the focus of communication with God, other people, self and environment. The quality of life of the subjects was evaluated before the intervention, immediately after the intervention and two months after that using The World Health Organization Quality of Life (WHOQOL) with mean score of 0 – 100.  FINDINGS: The results showed a significant difference between the dimensions of quality of life in the control and intervention groups: total health and quality of life (57.61±9.42) vs. (60±19.51) (p=0.002), physical health (46.22±19.53) vs. (61.35±13.36) (p=0.016), mental health (57.85±24.16) vs. (67.55±13.24) (p=0.005), social health (54.88±24.44) vs. (62.79±18.35) (p<0.001), and environmental health (62.05±3.55) vs. (67.33±33.43) (p=0.048), indicating that the results were stable over time. CONCLUSION: The results of the study showed that religious–spiritual psychotherapy increases the quality of life of women with breast cancer. 8211 Midwifery Vaginal Leech Infestation: a Rare Cause of Prepubertal Vaginal Bleeding and Anemia Saber A Khodaparast M Yazdani M 1. Department of Midwifery, Bojnurd Faculty of Nursing and Midwifery, North Khorasan University of Medical Sciences, Bojnurd, I.R.Iran 2. Research Unit, Bentolhoda Hospital, North Khorasan University of Medical Sciences, Bojnurd, I.R.Iran 3. Department of Obstetrics and Gynecology, Bentolhoda Hospital, North Khorasan University of Medical Sciences, Bojnurd, I.R.Iran 1 12 2019 21 1 74 77 05 10 2018 06 03 2019 BACKGROUND AND OBJECTIVE: Abnormal vaginal bleeding under the age of 10 is rare among girls. In such cases, local lesions, trauma, intra–vaginal foreign body, malignant genital tumors, and precocious puberty should be taken into account. Leech infestation is one of the rare causes of severe prepubertal vaginal bleeding. One case of prepubertal vaginal bleeding due to leech infestation is presented here. CASE REPORT: An eight–year–old girl referred to the emergency department after three days of painless vaginal bleeding. The bleeding was moderate during the first two days and the patient experienced intensified bleeding and large menstrual clots on the third day. The patient had no history of trauma, coagulation disorder, systemic illness, and drug use, but noted the history of swimming in the muddy creek within a few days before referral to the emergency department. After examining the genital system, a black mass with wavelike movements was observed in the lower one-third of the vagina, and a piece of it was slightly out of the hymen. The leech was removed by forceps without damaging hymen. After a short time, the bleeding stopped. On the next visit, the patient had no vaginal bleeding and was discharged without any problem. CONCLUSION: In cases of prepubertal vaginal bleeding, accurate examination of the genital system and evaluating the history of swimming or bathing in contaminated creeks, springs, qanats and pools are essential. 8142 Midwifery Effect of Attachment Behaviors Training on Maternal Competence attainment in Nulliparous Adolescent Women Jahdi F Kaheh A Haghani H 1.Nursing Care Research Center, School of Nursing and Midwifery, Iran University of Medical Sciences, Tehran, I.R.Iran 2.School of Nursing and Midwifery, Iran University of Medical Sciences, Tehran, I.R.Iran 3.Department of Biostatistics, School of Medical Management & Information, Tehran University of Medical Sciences, Tehran, I.R.Iran 1 12 2019 21 1 78 84 05 09 2018 06 03 2019 BACKGROUND AND OBJECTIVE: Adolescent pregnancy is a public health issue that requires knowledge, motivation, and skills, and the lack of it leads to a deficiency in mother competence. The aim of this study was to determine the effect of attachment behaviors training on maternal role competence in nulliparous adolescent women. METHODS: This clinical trial study, was conducted on 73 nulliparous Adolescent women at 28-32 gestational age attending the Educational centers dependent on Iran University of Medical Sciences. They were randomly divided into intervention and control groups. Three sessions of group training were held for mothers of interventional groups regarding Fetal Attachment Behaviors (Including counting the movements of the fetus and registration it, imagining the appearance of the fetus, talking with the fetus, and etc.). The control group received usual care. The Parenting Sense of Competence Scale questionnaire (score range: 17-102) was completed and assessed before the intervention and 10 days after delivery. FINDINGS: There was no statistically significant difference between the groups before the intervention in terms of the total score of Parenting Sense of Competence. 10 days after delivery, the mean score of maternal competence was 60.86 ±9.75 and 44.44±12.99 which was significantly higher compared to the control group (p<0.001). CONCLUSION: The results of this study showed that attachment behaviors training is effective in obtaining the competence of adolescent mothers.   7952 Midwifery The Relationship between Mothers’ Empowerment in Breastfeeding with Exclusive Breast Feeding in Infants Taheri Z Bakouei F 1. Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran. 2. Infertility and Health Reproductive Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran. 1 12 2019 21 1 85 92 18 06 2018 28 01 2019 BACKGROUND AND OBJECTIVE: Exclusive breastfeeding until 6 months has a positive effect on the maternal and the child health. In the Qur'an had also been mentioned to the nutrition of infant with breastfeeding. Knowing the effective factors on it has significant role in promoting exclusive breastfeeding policy. This study aimed to investigate relationship between the mothers’ empowerment in breastfeeding with exclusive breastfeeding and identification of related demographic-reproductive factors. METHODS: The cross-sectional study was done on 370 mothers with a six-month-old child admitted to the primary health care centers in Noor Township. Mothers were categorized according to type of breastfeeding to two the exclusive breastfeeding and the non-exclusive breastfeeding groups. The mothers’ empowerment in breastfeeding was assessed by breastfeeding empowerment questionnaire. The questionnaire includes seven domains of knowledge, attitude, skills of proper breastfeeding technique, skills of preventing and solving breastfeeding problems, breastfeeding sufficiency, receiving family support and Breastfeeding self-efficacy with probability total score in range of 45 to 225. FINDINGS: The mean total score of the mothers’ empowerment domains in breastfeeding in the exclusive breastfeeding group (202.70±13.12) is significantly higher than the non-exclusive breastfeeding group (160.59±27.82) (p=0.015). In the final analysis after entering variables in the logistic regression model, the breastfeeding empowerment’s domains including; attitude (OR=1.688, CI-95%=1.137-2.506, p=0.009), skills of proper breastfeeding technique (OR=1.471, CI-95%=1.078-2.007, p=0.015), skills of preventing and solving breastfeeding problems (OR=1.521, CI-95%=1.099-2.106, p=0.011), breastfeeding sufficiency (OR=5.150, CI-95%=2.707-9.795, p=0.001), receiving family support (OR=1.636, CI-95%=1.184-2.260, p=0.003) and Breastfeeding self-efficacy (OR=1.113, CI-95%=1.088-1.139, p=0.001) were found as significant related factors.   CONCLUSION: The findings of the study showed that knowledge alone cannot increase the prevalence of breastfeeding, but should also focus on other domains of mothers' empowerment, especially the belief to breastfeeding sufficiency in mothers. 7988 Midwifery Comparison of Vaginal Cream of Coconut Oil and Clotrimazole on Candidal Infection of Vagina Sheidaei S Jafarnejad F Rajabi O Najafzadeh MJ 1.Department of Nursing and Midwifery, Mashhad University of Medical Sciences, Mashhad, I.R.Iran 1.Department of Nursing and Midwifery, Mashhad University of Medical Sciences, Mashhad, I.R.Iran 2.Department of Pharmaceutical Control, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I.R.Iran 3.Department of Parasitology and Mycology, Mashhad University of Medical Sciences, Mashhad, I.R.Iran 1 12 2019 21 1 93 98 06 07 2018 23 01 2019 BACKGROUND AND OBJECTIVE: Candidiasis is the second common vaginal infection, and commonly used drugs are azoles. Over-consumption of Azol is associated with resistance to drug and treatment failure. Studies conducted in vitro confirm the antifungal effects of coconut oil. The aim of this study was to compare the effect of vaginal cream of coconut oil and clotrimazole on vaginal candidiasis infection. METHODS: This clinical trial study was performed on 71 women with Candida infection in two groups receiving vaginal cream of coconut oil: 35(78%) and clotrimazole (36%) (1%) who were treated for 7 nights (one applicator per night). One week after the completion of the treatment, patients were re-examined. Itching, burning, pain and secretion were compared in two groups. FINDINGS: After the mean intervention (itching in the coconut group was 0±0 and in the clotrimazole group was 19±0.46 (p=0.017), (burning in the coconut group was 0.08±0.20 and in the clotrimazole group was 57% (p=0.046), (secretion in the coconut group was 0.05±0.23 and in the clotrimazole group was 66.6 ± 0.98 (p=0.001) (pain during sex in the coconut group 23 0.05 and 0.30±0.62 in the clotrimazole group (p=0.031). However, the success of the treatment (negative culture) was not significant between the two groups (p<0.05). CONCLUSION: The present study showed that vaginal cream of coconut oil and clotriamzole both improve vaginal candidiasis infection. Therefore, vaginal cream of coconut oil can be used as an alternative treatment for vaginal candidiasis. 8302 Obstetrics and Gynecology Hysteroscopic and Pathologic Evaluation of Chronic Endometritis in Patients with Unexplained Recurrent Spontaneous Abortion Barat Sh Adnani M Basirat Z Ranaei M 1. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 2. Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran 1. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 1. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 1 12 2019 21 1 99 104 10 11 2018 12 05 2019 BACKGROUND AND OBJECTIVE: Chronic endometritis is one of the most specific, but not the most common causes of infertility, which reduces the amount of pregnancy and poor results of pregnancy, such as preterm labor and abortion. The aim of this study was to evaluate the role of chronic endometritis in recurrent spontaneous abortions (RSA) and to determine the bilateral relationship between the histological and pathological findings of this inflammation. METHODS: A case controlled-prospective observational study was performed on one hundred women 20-35 years in the Fatemeh Zahra infertility center and Rohani hospital. Patient group include forty patients with unexplained RSA and control group include 60 women that underwent hysteroscopy due to vaginal bleeding or other causes except RSA. All of the women underwent endometrial biopsy and examined by pathologist for presence plasma cells in stroma. FINDINGS: In all patients endometritis rate was 8%. Patients with RSA had a significantly higher incidence of CE both hysteroscopically (30% vs. 6.7%; p<0.005) and pathologically (27.5% vs. 6.7%; p < 0.005). The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of hysteroscopy in the diagnosis of CE were 93.3%, 97.6%, 87.5% and 98.8% respectively. Also the PPV and NPV of hysteroscopy, sensitivity, specificity in the diagnosis of endometrial polyp were 100%, 87.3%, 76.3% and 100% respectively. CONCLUSION: Based on the results, there was a significant association between CE and unexplained RSA. Due to high sensitivity and acceptable specificity of hysteroscopy in diagnosis of CE and endometrial polyp, we recommended hysteroscopic evaluation of patients with unexplained RSA. 7879 Microbiology Rapid Identification of Enterococcus Faecalis Isolates by Polymerase Chain Reaction Sabzi S Ohadian Moghaddam S Pakbaz Z Pourmand MR 1. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, I.R.Iran 2. Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, I.R.Iran 3. Urology Research Center, Tehran University of Medical Sciences, Tehran, I.R.Iran 4. Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, I.R.Iran 1 12 2019 21 1 105 110 23 05 2018 09 04 2019 BACKGROUND AND OBJECTIVE: Rapid detection of Enterococcus faecalis as a frequent cause of nosocomial infections in immunocompromised patients is an important issue. Herein, the current study developed a PCR assay based on the ef0737 gene to detect E. faecalis isolates. METHODS: In this cross-sectional study, 150 clinical isolates including E. faecalis, E. faecium and Staphylococcus aureus were collected. A set of pair primers was designed using the sequence of ef0737. All isolates were examined for the presence of ef0737 gene by PCR assay. The sensitivity of PCR assay was evaluated according to 50 clinical isolates of E. faecalis. The specificity of PCR primers was also determined using non-E. faecalis species including 50 E. faecium and 50 S. aureus isolates. FINDINGS: In this study, from 150 clinical isolates that were collected; all the 50 E. faecalis isolates showed positive results for the ef0737 gene which showed 100% sensitivity. No amplification were observed in other isolates include E. faecium and S. aureus. CONCLUSION: PCR assay is a more efficient and sensitive tool for detection and characterization of E. faecalis especially in patients with the critical condition. Identification of the preserved ef0737 gene in clinical samples may be able to determine infections caused by E. faecalis. 7954 Psychiatry A Case Report of Opioid Induced Hypomania and Obsessive Compulsive Disorder Hashemi SH Hosseini SH 1. Iranshahr University of Medical Sciences, Nic-shahr, I.R.Iran 2. Psychiatry and Behavioral Sciences Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, I.R.Iran 1 12 2019 21 1 111 115 20 06 2018 09 04 2019 BACKGROUND AND OBJECTIVE: Obsessive compulsive disorder (OCD) and bipolar disorder are common psychiatric illnesses and there are many challenges in pathology and their treatment. In recent years, the role of opioid system in the pathology of these disorders has been considered. In this report, we present a case with simultaneous episodes of hypomania and OCD following opium use. CASE REPORT: The 65-year-old man, who was suffering from irritability, talkativeness, over spending and frequent washing following use of opium, visited in psychiatric clinic. After administration of 30 mg of methadone every day, all her symptoms improved. During 12 years of follow-up, we observed that her symptoms recurred when the patient was taking opium again. Few days after repeat prescription of methadone, these symptoms subsided. CONCLUSION: The findings of this study emphasize the role of opioid system in the pathology of obsessive-compulsive disorder and bipolar disorder, at least when there are comorbid. 8084 Genetics, Cell and Molecular Biology Cytotoxic Effect of Titanium Dioxide-Hydroxyurea Nanocamposit on Hela Cancer Cell Line Ghahremani Farasfaji R Bikhof Torbati M Tafvizi F Shaabanzadeh M 1. Department of Biology, Yadegar-e-Imam Khomeini Shahr-e-rey Branch, Islamic Azad University, Tehran, I.R.Iran 1. Department of Biology, Yadegar-e-Imam Khomeini Shahr-e-rey Branch, Islamic Azad University, Tehran, I.R.Iran 2. Department of Biology, Parand Branch, Islamic Azad University, Parand, I.R.Iran 3. Department of Chemistry, Damghan Branch, Islamic Azad University, Damghan, I.R.Iran 1 12 2019 21 1 116 121 09 08 2018 22 04 2019 BACKGROUND AND OBJECTIVE: The use of nanotechnology in drug delivery Not only increase the efficacy and ease of drug penetration, but also they decrease their adverse effects. In this study, TiO2 nanoparticle was used as Hydroxyl Urea carrier to increase the contact surface of the drug and cells, and with pegylation of nanoparticle surface decrease immunogenicity, hence to increase drug solubility and penetration to cells. The goal of this study was to investigate cytotoxicity of synthesized TiO2-Poly Ethylene Glycol-Hydroxy Urea (TiO2-PEG-HU) nanocomposite on Hela cell-line and apoptosis induction of treated cells compared to the control group to determine the effective dose of nanodrug. METHODS: In this laboratory study, the effect of TiO2-PEG-HU nano-drug was evaluated on cells bioactivity by MTT method at concentrations of 200, 400, 800, and 1800 µg/ml in 48 and 120 hours. Annexin-V/PI flowcytometry method was used to analyze apoptosis induction. Data were analyzed using uni-directional variance and independent T-test. FINDINGS: Higher concentrations of TiO2-PEG-HU nanocomposite decreased cells bioactivity dependent on dosage and time. As the concentration of 1600 µg/ml of nanocomposite reduced amount of bioavailability by 1.52 times over a 120-hour period compared to the 48-hour time-effect. For both times, this reduced cell survival was `significantly different from that of the control group at the level of p <0.0001. In addition, nano-drug significantly increased apoptosis induction 2.5 times in treated Hela cells (p=0.0114). CONCLUSION: Nano-composite TiO2-PEG-HU on the Hela cell line is cytotoxic and induces apoptosis and can be a promising drug for cancer treatment. 8113 Dentistry (pediatrics) Report a Case of Beauty Regeneration in a Child with Premature Tooth Decay Alinejhad D Amrollahi N 1. Department of Pediatric Dentistry, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, I.R.Iran 2. Dental Research Center, Faculty of Dentistry, Isfahan University of Medical Sciences, Isfahan, I.R.Iran 1 12 2019 21 1 122 126 26 08 2018 06 03 2019 BACKGROUND AND OBJECTIVE: One of the biggest challenges in pediatric dentistry is the oral rehabilitation of children who have lost their teeth due to early childhood decay severe trauma. In this report, a fixed anterior plaque is reported in a child with early decay. CASE REPORT: In this study, a 4-year-old boy referred to a dental clinic in Isfahan with a major complaint of ugly appearance and inability to chew on anterior teeth due to decay. After clinical and radiographic examinations of the posterior teeth, pulp treatment and stainless steel coating were applied and the anterior teeth were pulled. After 6 weeks of complete recovery of the wound site, the cords with appropriate size fitted to the second molar maxillary teeth and matched to the upper jaw. After making the appliance and before the cementation, the plaque was examined for oral occlusion and beauty, with the help of Fuji I.GC corporation cement fixed in the mouth. The child was called 24 hours later, then one month later, and was followed up regularly by the age of seven. CONCLUSION: According to the results of this reported case, the creation of a fixed anterior plaque in the child not only does not cause wounds and discomfort in the child's mouth, but also improves the ability of speech and beauty. 8049 Microbiology Role and Function of KPC and MBL Enzymes in Increasing the Pathogenicity of Pseudomonas Aeruginosa Isolated from Burn Wounds Tahmasebi H Maleki F Dehbashi S Arabestani MR 1. Department of Microbiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, I.R.Iran 2. Department of Microbiology, Faculty of Basic Sciences, Hamadan University of Basic Azad University, Hamadan, I.R.Iran 3. Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, I.R.Iran 4. Brucellosis Research Center, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, I.R.Iran 1 12 2019 21 1 127 134 29 07 2018 25 03 2019 BACKGROUND AND OBJECTIVE: Pseudomonas aeruginosa is one of the main causes of hospital infections. Pathogenic factors in this bacterium may play a role in the resistance to carbapenem and beta-lactam. The purpose of this study was to evaluate the role and function of KPC and MBL enzymes in increasing the pathogenicity of Pseudomonas aeruginosa isolated from burn wounds. METHODS: In this cross-sectional study, 63 isolates of Pseudomonas aeruginosa from burn wounds of different patients were isolated using biochemical tests such as fermentation of sugars in the OF medium, oxidase test, and so on. Determination of resistance pattern and strains with metallobetalactamase and carbapenema was done by disc diffusion method. The oprD gene was used for molecular confirmation of isolates. PCR method was used to detect pathogenicity genes. FINDINGS: Out of 63 isolates of Pseudomonas aeruginosa isolated from burn patients, 10 isolates (15.83%) had KPC enzyme and 13 isolates (20.63%) had MBL enzymes. Doripenem, Ertapenem and meropenem were the most frequent. Also, the lasB gene was observed in 43 isolates (68.25%), plcN gene in 41 isolates (65.07%), lasA gene in 20 isolates (31.74%), apr in 60 isolates (95.23%), phzI gene in 53 isolates (84.12%), the phzII gene in 38 isolates (60.31%), phzH gene in 30 isolates (47.61%) and plcH gene in 56 isolates (88.88%). CONCLUSION: The results of this study showed that the production of Carbapnemase and MBL enzymes increased the pathogenicity of Pseudomonas aeruginosa isolated from burn wounds. 8256 Children\'s Blood Serum αFP Level in Cord Blood of Full Term Neonates Born in Babol City Zahedpasha Y Tamaddoni A Ahmadpour-kacho M Pourmoghtader S Non-Communicable Pediatric Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran Non-Communicable Pediatric Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran Non-Communicable Pediatric Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran Non-Communicable Pediatric Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 1 12 2019 21 1 135 139 20 10 2018 09 04 2019 BACKGROUNDANDOBJECTIVE: Serum alpha-fetoprotein (αFP) level is considered as a diagnostic marker is higher than normal in many congenital tumors such as germ cell, hepatoblastoma, as well as liver and metabolic diseases in neonates. Normal neonates also have a higher level of alpha-fetoprotein than others, so it is important to diagnose this interference. In valid sources, the normal serum alpha-fetoprotein level in infants is related to advanced countries, which may vary in our country. Therefore, this study was conducted to determine the serum levels of alpha-fetoprotein in the umbilical cord blood of term neonates born in Babol and to compare them in two genders. METHODS: This cross-sectional study was performed on 500 neonates (37-42 weeks) born in hospitals in Babol city where physical examination was normal. At birth, 5 ml of umbilical cord blood was taken and samples were sent to the lab for measurement of alpha-fetoprotein. Serum alpha-fetoprotein level was measured by ELISA method and was compared in two genders. FINDINGS: Mean serum a FP levels was 76.57±35.25 ng/ml (range 2.3-160) and it was significantly higher in males (80.54±36.95 vs. 73.69±33.73 ng/ml) which was statistically significant (p=0.002). CONCLUSION: The results of this study showed that the level of alpha-fetoprotein in neonates born in Babol is relatively high and also in males is more than females. 7987 Parasitology Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients Shokri F Abbaszadeh R Rrostamnezhad M Heydari digeh sara S Marandi N Bairami kuzeh konan A Abolhasani M Gharavi MJ 1. Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 2. Department of Medical Parasitology, School of Medicine, Alborz University of Medical Sciences, Karaj, I.R.Iran 3. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, I.R.Iran 4. Department of Radiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, I.R.Iran 5. Department of Medical Parasitology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I.R.Iran 2. Department of Medical Parasitology, School of Medicine, Alborz University of Medical Sciences, Karaj, I.R.Iran 6. Department Of Epidemiology, School of Public Health, Iran University of Medical Sciences, Tehran, I.R.Iran 2. Department of Medical Parasitology, School of Medicine, Alborz University of Medical Sciences, Karaj, I.R.Iran 1 12 2019 21 1 140 146 06 07 2018 10 04 2019 BACKGROUND AND OBJECTIVE: Toxoplasma gondii is associated with several complications including neurological problems, ocular damage and encephalitis in immunodeficiency individuals. Early diagnosis of this infection can lead to better management of this disease. Therefore, this study was conducted to determine the presence of Toxoplasma gondii with two serologic and molecular methods in HIV-infected individuals. METHODS: In this cross-sectional study, 102 male patients with HIV with a mean age of 40 ± 9.2 years were examined. The serum sample was used for ELISA to determine the acute and chronic phase and cellular samples using Real Time-PCR for determining the acute phase of the disease. The relationship between age groups and the HIV transmission pathway, as well as the age group, was compared with the results of the Toxoplasma gondii test. FINDINGS: Out of 102 samples tested for IgM anti-Toxoplasma gondii antigen by ELFA, all (100%) samples were negative, but for anti-IgG anti-parasite, 44 samples (43.1%) were positive and 58 Sample (56.9%) was negative. Out of 102 samples tested by RT-PCR, all (100%) samples were negative for Toxoplasma DNA. There was a statistically significant relationship between age groups and transmission pathways (p<0.001), as well as between age groups with anti-Toxoplasma gondii IgG levels (p<0.001). CONCLUSION: According to the results of this study, the use of IgM-ELFA and PCR-RT methods for the diagnosis of acute phase and IgG-ELFA in the chronic phase of the disease is important. With the diagnosis of chronic form of toxoplasmosis, preventive treatments can be used in HIV + patients.   7637 surgery Clinicopathologic Characteristics of Multifocal Gastric Adenocarcinoma Kalbasi M Siadati S Kamali Ahangar S Nikbakhsh N 1. Student Research Committee, Babol University of Medical Science, Babol, I.R.Iran 2. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 3. Clinical Research Development Center, Shahid Beheshti Hospital, Babol University of Medical Sciences, Babol, I.R.Iran. 4. Cancer Research Center, Health Research Institute, Babol University of Medical Sciences, Babol I.R.Iran 1 12 2019 21 1 147 152 28 02 2018 09 04 2019 BACKGROUNDANDOBJECTIVE: Gastric cancer is the fifth prevalent cancer in worldwide. Among of this, adenocarcinoma is more than 95% of all case and most prevalent. According to gastric adenocarcinoma is resistant to adjuvant therapy, surgical resection is the cardinal strategies of treatment. One of the problems with treating adenocarcinoma of the stomach is that its main causes are malignant or pre-malignant changes in the residual gastric tissue that may be neglected during resection. By recognizing these lesions and resection, they can be expected to reduce the malignancy. Therefore, this study was conducted to investigate the characteristics of non-tumoral gastric tissue in terms of malignant or pre-malignant changes. METHODS: In this retrospective cross-sectional study, 112 patients who had undergone total gastrectomy in the hospitals affiliated to Babol University of Medical Sciences during the period of 2013-2017 were examined and analyzed for age, sex, type and location of tumor. FINDINGS: Of 112 cases that studied,72 cases (64.3%) were male and 40 cases (35.7%) were female. The mean of ages was 64.7±8.6 year. Poorly differentiated pathology in 60.7% and well differentiated pathology in 39.3% of patients were seen. In 64 patients (57%), there was synchronous lesions in addition to primary pathology. These synchronous lesions were related statistically significant to pathology, cancer stage and gender. CONCLUSION: Based on the results of this study, it can be concluded that resection should be accompanied by considering simultaneous lesions in addition to primary pathology, which can be a reason for total gastrectomy as a standard treatment for gastric cancer. 8120 Midwifery Pyogenic Granuloma in Pregnancy: A Case Report Naghi I Keypour F 1. Department of Obstetrics & Gynecology, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 2. Department of Obstetrics & Gynecology, Akbarabadi Teaching Hospital, Iran University of Medical Sciences, Tehran, I.R.Iran 1 12 2019 21 1 153 156 27 08 2018 30 07 2019 BACKGROUND AND OBJECTIVE: Pyogenic granuloma is a lesion that affects pregnant women between the third and ninth month of pregnancy. It is a benign vascular proliferative disease that often appears on the gingiva, but may also appear on the lips, skin and extramucosal tissues. The purpose of the report is to introduce a pregnant woman with pyogenic granuloma with a lesion on her face. CASE REPORT: The patient is a pregnant woman at 39 weeks of gestation without a history of a specific illness who referred to Shahid Akbarabadi Hospital in Tehran due to labor pain. A vascular nodule was visible on her face, which appeared from the first half of pregnancy and was reported to be normal in terms of paraclinical experiments. This vascular nodule improved after delivery but did not completely disappear. The patient underwent aesthetic surgery for the vascular nodule three months after delivery. The results of the pathology confirmed pyogenic granuloma. CONCLUSION: According to the results of this report, if unusual lesions are observed on the face during pregnancy, oral and dental problems should be considered and patients should be examined by the dentist. 7863 Genetics, Cell and Molecular Biology The Prevalence of the Genetic Polymorphism of GSTM1, GSTT1 and GSTP1 and Its Relationship with Clinical Criteria of Multiple Sclerosis (MS) Patients in Tehran Aliomrani M Sahraian MA Khoshayand MR Danesh Seta T Sharifzadeh M Ghahremani MH 1. Department of Toxicology and Pharmacology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, I.R.Iran 2. Department of Neurological Disorder, School of Medicine, Tehran University of Medical Sciences, Tehran, I.R.Iran 3. Department of Drug and Food Control, School of Pharmacy, Tehran University of Medical Sciences, Tehran, I.R.Iran 1. Department of Toxicology and Pharmacology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, I.R.Iran 4. School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, I.R.Iran 4. School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, I.R.Iran 1 12 2019 21 1 157 165 19 05 2018 23 01 2019 BACKGROUND AND OBJECTIVE: Multiple Sclerosis is the chronic inflammation of central nervous system with demyelinated lesions in the brain and spinal cord. The genetic polymorphisms associated with glutathione S-transferase enzymes involved in antioxidant defense in Iranian patients have not been investigated. Therefore, in the present study, the prevalence of the genetic polymorphism of glutathione S-transferase M1, P1 and T1 and its relationship with clinical criteria of MS patients with has been examined. METHODS: In this case-control study, 69 patients who referred to Sina Hospital in Tehran and had no panic attack within the last three months and 74 healthy subjects were interviewed. After examination by neurologist and blood sampling, DNA extraction was performed using Roche kit. Then, the genotypic variations of the samples were evaluated using RFLP-PCR and its prevalence was analyzed in relation with age, birth weight, malignancy (EDSS) and gender using GraphPad Prism software. FINDINGS: Most malignancies were observed in men (3.1±5.9) and the highest incidence rate was observed in those born in May (30%). Although the results of genotyping between the studied groups and their gender did not show any significant difference (OR: 2-4, p>0.05), patients with GSTM1 deficiency developed the disease at a lower age (32.8±2.6 years) compared with other patients (29.5±8.9 years) (CI-95%: 20.3–26.4, p=0.009). In addition, people with a rare GSTM1 allele who smoked cigarette had higher EDSS (CI-95%: 2.1–3.7, p=0.03). CONCLUSION: Based on the results of this study, the effect of GSTM1 on malignancy is indicative of its role in detoxification of tobacco products and can be used as an agent for early diagnosis of disease in people who are susceptible to this disease.   8260 Obstetrics and Gynecology The Risk Factors for Ectopic Pregnancy Bouzari Z Yazdani ُSh Alizadeh M Ghanbarpour A Bijani A Lakaei-andy F 1. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 2. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 3. Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran 4. Clinical Research Development Unite of Rouhani Hospital, Babol University of Medical Sciences, Babol, I.R.Iran 5. Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 4. Clinical Research Development Unite of Rouhani Hospital, Babol University of Medical Sciences, Babol, I.R.Iran 1 12 2019 21 1 166 173 21 10 2018 06 03 2019 BACKGROUND AND OBJECTIVE: Ectopic pregnancy is a common complication and one of the causes of pregnancy-related deaths. Considering the increased prevalence of ectopic pregnancy in recent years and the increased risk factors such as assisted reproductive treatment, the present study was conducted to investigate the risk factors for ectopic pregnancy in Babol, northern Iran. METHODS: This case-control study was performed on pregnant women who were admitted with suspicion for ectopic pregnancy or acute abdomen within a 6-year period. Patients diagnosed with ectopic pregnancy were selected in the case group and the control group was selected from among the pregnant women's medical record during the same years. Patients were examined in terms of age, parity, history of abortion, type of contraception, history of ectopic pregnancy, history of pelvic infections, history of cesarean section, infertility treatment, history of smoking, marriages within the family, medical history and underlying disease. FINDINGS: In this study, 201 patients were examined in each group. The mean age for the case group was 29.75±5.6 and for the control group was 28.62±6.4 years. Among the risk factors, assisted reproductive treatment (OR=10.24, p≤0.001), abdominal and pelvic surgery (OR=2.35, p=0.002), infertility (p=6.76, p<0.001), contraceptive pills (OR=0.61, p<0.001) and nulliparity (OR = 1.61, p=0.019) were associated with increased risk of ectopic pregnancy. CONCLUSION: According to the results of this study, the most important risk factors for ectopic pregnancy are the use of hormonal pills to prevent pregnancy and nulliparity, respectively. 8324 Medical Physics Validating the Dosimetric Parameters of INTRABEAM Intraoperative Radiotherapy Machine Baghani HR Ghorbani Zh Akbari ME 1. Department of Physics, Faculty of Sciences, Hakim Sabzevari University, Sabzevar, I.R.Iran 2. Shahid Beheshti University, Tehran, I.R.Iran 3. Cancer Research Center, Shahide Beheshti University of Medical Sciences, Tehran, I.R.Iran 1 12 2019 21 1 174 180 21 11 2018 22 04 2019 BACKGROUND AND OBJECTIVE: One of the methods for treating breast cancer is radiotherapy using low-kV X-rays in which a dedicated device called INTRABEAM is used along with a few spherical applicators for breast radiation. Due to the single-session nature of this treatment, evaluating the accuracy of the device used for treatment is essential. The aim of this study was to evaluate the dosimetric parameters of the INTRABEAM system with spherical applicators and validate the reported results for clinical use in intraoperative radiotherapy for breast tumors. METHODS: In this study, dosimetric parameters including percentage depth dose curve (PDD), transfer function (TF) and anisotropy were determined by MCNPX Monte Carlo Simulation Tool and practical dosimetry was done by Gafchromic EBT2 film. The results were quantitatively compared with the results reported by manufacturer of the device (Carl Zeiss) to evaluate the accuracy of the reported data for this treatment system.   FINDINGS: The mean difference when comparing PDD curves was 1.7% and the mean difference between the compared TF values was about 2%. The anisotropy values obtained by Monte Carlo Simulation and Gafchromic EBT2 film were also within the range recommended by the manufacturer.  CONCLUSION: Based on the results, it can be concluded that the dosimetric parameters reported by the manufacturer for spherical applicators of the INTRABEAM system are valid for designing the treatment and radiotherapy for patients. 8241 Geriatric Health The Effect of Citrus Aurantium Aroma on Sleep Quality in the Elderly with Heart Failure Arab Firouzjaei Z Illali ES Taraghi Z Mohammadpour RA Amin K Habibi E 1. Student Research Committee, Mazandaran University of Medical Sciences, Sari, I.R.Iran 2. Health Sciences Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, I.R.Iran 2. Health Sciences Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, I.R.Iran 3. Department of Biotatistics, School of Health, Mazandaran University of Medical Sciences, Sari, I.R.Iran 4. Clinical Research Development Unite of Rouhani Hospital, Babol University of Medical Sciences, Babol, I.R.Iran 5. Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, I.R.Iran 1 12 2019 21 1 181 187 14 10 2018 28 04 2019 BACKGROUND AND OBJECTIVE: Poor sleep quality is one of the most common problems in the elderly with heart failure, and hospitalization exacerbates this problem. In traditional Persian medicine, citrus aurantium has been recognized as a sedative and inducer of sleep. The aim of this study was to determine the effect of citrus aurantium on sleep quality in the elderly with heart failure. METHODS: This randomized clinical trial was performed on 80 elderly patients with heart failure admitted in selected teaching hospitals of Mazandaran province in two intervention and control groups. In the intervention group, aromatherapy with two drops of 10% citrus aurantium essential oil was performed on a cotton ball for twenty minutes for three consecutive nights and the control group received routine care. Sleep quality of the elderly was assessed before and after the intervention using the standard St. Mary's Hospital Sleep Questionnaire (SMHSQ) (which assesses the quality of previous night's sleep in hospitalized patients).   FINDINS: The mean sleep disorder in the elderly before and after the intervention in the intervention group was 24.70±3.67 and 21.05±3.62, respectively, which was statistically significant (p=0.001). Mean sleep disorder in the elderly before and after the intervention in the control group was 24.37±4.48 and 23.75±4.71, respectively, which was not statistically significant. CONCLUSION: The results showed that using aromatherapy with citrus aurantium can improve sleep quality in the elderly with heart failure. 8435 Pharmacology The effect of hydro-alcoholic extract of Kelussia odoratissma Mozaffarian (KOM) seed on histopathology & joints diameter in experimental model of Incomplete Freund,s adjuvant induced arthritis in rat Hoseyni Largani M Rashidpour Aghamahali M Kazemi S Hosseini SM Rafieian Kopaei M Feyzi F Moghadamnia AA 1. Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran 1. Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran 2. Cancer Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 3. Department of Veterinary Parasitology, Babol-Branch, Islamic Azad University, Babol, I.R.Iran 4. Medicinal Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, I.R.Iran 5. Neuroscience Research Center, Health Research Institute, Babol University of Sciences, Babol, I.R.Iran 6. Cellular and Molecular Biology Research Center, Health Research Center, Babol University of Medical Sciences, Babol I.R.Iran 1 12 2019 21 1 188 195 05 01 2019 15 06 2019 BACKGROUND AND OBJECTIVE: Arthritis is one of the inflammatory diseases that can cause debilitating problems. Existing drugs have numerous side effects that limit their use. KOM has anti-inflammatory properties due to its flavonoid compounds. Therefore, this study was performed to investigate the anti-inflammatory effects of hydroalcoholic extract of KOM in an experimental model of arthritis. METHODS: In this experimental study, 46 male Wistar rats were randomly divided into 7 groups: healthy and patient control groups, normal saline recipient , hydrocortisone recipient and 3 groups treated with hydroalcoholic extract at doses of 300, 500 and 700 mg/kg. First, KOM was extracted then arthritis induced by injection of 0.1 cc of Incomplete Freund's adjuvant, and from 15th day KOM extract was injected intraperitoneally. On the last day (31st), their ankle joint was prepared for histological examination and the groups were compared. FINDINGS: The results showed that doses of 300, 500 and 700 mg/kg of hydroalcoholic extract did not significantly reduce joint diameter. Histologic studies of positive control group showed subcutaneous inflammation, destruction and fibrosis of cartilage and panus formation which subcutaneous inflammation was reduced in the low and high doses of the extract (p<0.05). CONCLUSION: The results of this study showed that hydroalcoholic extract of KOM reduced subcutaneous inflammation but had no significant effect on joint diameter and other histopathologic and immunological changes in IFA induced arthritis. 8249 Physiology Evaluation of Hydroalcoholic Extract of Cinnamon Effect on Testicular Tissue and Fertility of Busulfan-Induced Oligo-Spermic Model Rats Soleimani P Chegini R Sadeghi M Younesi F Zafari F 1. Student Research Committee, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, I.R.Iran 1. Student Research Committee, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, I.R.Iran 2. Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, I.R.Iran 1. Student Research Committee, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, I.R.Iran 3. Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, I.R.Iran 1 12 2019 21 1 196 200 16 10 2018 25 06 2019 BACKGROUND AND OBJECTIVE: Busulfan is one of the cancer treatment drugs that cause infertility of the patient. In traditional medicine, one of the important properties of cinnamon is enhancement of fertility. The aim of this study was to investigate the effect of cinnamon on the physiological structure of testes and fertility rate of busulfan-treated rats. METHODS: In this experimental study, 24 adult male rats were divided into 3 groups of 8 subjects, Sham: healthy rats without intervention. Control group: Busulfan-sterilized rats (15 mg/kg) intraperitoneal injection, Cinnamon group: Busulfan-treated rats + cinnamon extract (200 mg/kg) for fourteen days. The testicles length, width and weight parameters as well as the formation of germinal cells were analyzed by H & E staining and optical microscopy. FINDINGS: The number of spermatogonia cells, primary spermatocytes and spermatid in the cinnamon group was 72.25±12.63, 61.71±11.47, and 79.1±10.02, and in the control group was 51.83±23.37, 31.67±5.27 and 38.67±15.7 respectively, and the increase in cinnamon group was significant (p<0.005). The testicles length, width and weight parameters in the cinnamon group were increased compared to the control group (busulfan). The thickness of germinal epithelium in the cinnamon and control group was 53.46 ± 16.44 and 30.4±10.21 respectively, which was significantly higher in the cinnamon group (p<0.001). CONCLUSION: According to the findings of this study, cinnamon extract has positive therapeutic and protective effects on testicular tissue and increases sperm production in busulfan-treated rats. 8720 Urology A Rare Case of Intravesical Foreign Body(Pencil): A Case Report Moudi E Darzi MM Kamalinia H 1. Cancer Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 2. Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran 2. Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran 1 12 2019 21 1 201 206 14 05 2019 17 08 2019 BACKGROUND AND OBJECTIVE: The presence of foreign body in the bladder is a rare occurrence, which is often caused by the exposure of the individual him- or herself, or remaining part of the catheter, and the migration of medical equipment from adjacent organs or by a penetrating wound. Here is a case of a pencil in the bladder of a woman with mental retardation. CASE REPORT: The patient is a 34-year-old woman who referred to a gynecologist with vague symptoms of abdominal and lower abdominal pain and a foreign body was detected in ultrasound on bladder. The patient was then referred to a urologist. There was no noteworthy point in the radiography. Computed tomography (CT) of the abdomen and pelvis was performed to confirm the nature, shape and position of the probable foreign body, and a 71 mm long object was observed. The patient then underwent cystoscopy and finally, after observation of the foreign body (which was a pencil), the pencil tip was fractured by a mechanical crusher and through the hole created in the tip of the pencil, it was held by a grasper and was removed longitudinally. The patient was discharged with oral antibiotics.  The symptoms were resolved and the patient had no complaints in the follow-up. CONCLUSION: Considering the case reported here, it is necessary to examine abdominal and pelvic pain or urinary symptoms (even nonspecific ones) in people with mental retardation in terms of the presence of foreign body in the bladder and pelvis. 8250 Microbiology Green Synthesis of Silver Nanoparticles Using the Extract of Lonicera Nummulariifolia and Investigating Its Antioxidant, Antimicrobial and Anticancer Effects Against Lung Cancer Cell Line A549 Rezaie H HAmdi SMM Mirzaie A 1. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, I.R.Iran 1. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, I.R.Iran 2. Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, I.R.Iran 1 12 2019 21 1 207 214 16 10 2018 09 04 2019 BACKGROUND AND OBJECTIVE: Due to the increasing incidence of cancer-related deaths and the deficiencies of chemotherapy and radiotherapy in advanced forms of cancer, new approaches are needed to control cancer, and one of these techniques is the use of nanoparticles, especially silver nanoparticles. Nowadays, the use of plants for the synthesis of silver nanoparticles has attracted the attention of researchers due to their low cost. Therefore, the aim of this study was to investigate the green synthesis of silver nanoparticles using the extract of lonicera nummulariifolia and to study its antioxidant, antimicrobial and anticancer effects against lung cancer cell line A549.  METHODS: In this experimental study, silver nanoparticles were synthesized using the extract of lonicera nummulariifolia as a regenerative agent. The antioxidant effects of synthesized silver nanoparticles were evaluated by DPPH assay and finally its antimicrobial and anticancer activity were respectively evaluated by Broth Microdilution and MTT assays at concentrations of 3.125, 6.25, 12.5, 25, 50, 100 μg/ml on lung cancer cell line within 24 h.  FINDINGS: The results of DPPH assay showed that the synthesized silver nanoparticles at 100 µg/ml had an antioxidant effect of 33.77±0.83. Antimicrobial test results showed that the antimicrobial effects of silver nanoparticles were greater on gram negative bacteria. MTT results also showed that cell viability was 70.33±0.21 (p>0.05), 51.66±0.24 (p<0.05) 35.75±0.35 (p<0.01), 20.66±0.28 (p<0.001), 13.5±0.31 (p<0.001), and 7.6±0.37 (p<0.001), respectively. Results of DPPH assay showed that silver nanoparticles has significant antioxidant effects (p<0.05). CONCLUSION: Considering the antimicrobial and anticancer effects of synthesized silver nanoparticles, it can be used as a drug candidate. 7921 Midwifery The Effect of Cognitive–Behavioral Group Therapy on Menopausal Symptoms Soori M Kolivand M Abolfathi Momtaz Y Noori P 1. Faculty of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, I.R.Iran 2. Department of Reproductive Health, Faculty of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, I.R.Iran 3. Malaysian Research Institute on Ageing (MyAgeing), Universiti Putra Malaysia, Serdang, Selangor, Malaysia 1. Faculty of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, I.R.Iran 1 12 2019 21 1 215 222 05 06 2018 09 04 2019 BACKGROUND AND OBJECTIVE: The common symptoms of menopause are associated with anxiety and discomfort for most women, and this is one of the major healthcare challenges. The aim of this study was to evaluate the effect of cognitive – behavioral group therapy on menopausal symptoms (primary outcome). METHODS: This randomized controlled clinical trial was conducted among 90 menopausal women with health records at two health centers in Tuyserkan in 2016 and were randomly assigned to two groups of intervention and control (45 patients in each group). To perform cognitive – behavioral group therapy, six 90-minute sessions were held for the intervention group for six consecutive weeks. Menopausal symptoms were discussed in each of these sessions based on cognitive techniques such as identifying negative automatic thoughts and behavioral techniques such as diaphragmatic breathing technique. Menopausal symptoms were assessed in both groups using the Greene Climacteric Scale (0 – 63) before the intervention and at the end of the sixth week. To adhere to ethics, the control group received one session of educational counseling after the assessments were done. FINDINGS: There was no statistically significant difference in mean total Greene score between the cognitive– behavioral group (22.78±12.22) and control group (24.8±10.25) before intervention. After the intervention, the mean total Greene score decreased significantly in the cognitive – behavioral group (15.75±7.24) compared to the control group (24.97±9.25) (p < 0.05). CONCLUSION: The results showed that cognitive – behavioral group therapy can decrease menopausal symptoms. 8966 Medical Ethics Presenting a Model of Impact of Research Ethics on the Quality of Research of Faculty Members Baleghi Damavandi S Zameni F Taghvaee Yazdi M 1. Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, I.R.Iran 1. Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, I.R.Iran 1. Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, I.R.Iran 1 12 2019 21 1 223 229 14 09 2019 05 10 2019 BACKGROUND AND OBJECTIVE: With the increasing growth of research in society and the importance and necessity of research in development and progress, adherence to ethics in research seems to be one of the most important pillars for the development of science. Therefore, the present study was conducted to present a model of the impact of research ethics on the quality of research of faculty members. METHODS: This cross-sectional study was performed on 320 faculty members of Medical Sciences Universities of a large area of the country (Babol, Semnan, Shahrood, Golestan, Gilan and Mazandaran) selected by stratified random sampling. In order to investigate the impact of research ethics on research quality, researcher-made questionnaires of research ethics consisted of 65 questions and two dimensions of "individual and social” and nine components (trustworthiness, responsibility, professional commitment, knowledge and perseverance and patience, respect for the rights of the subjects, teamwork morale, responsibility for disseminating results, and attending to community needs) were used. In addition, the research quality questionnaire including 31 questions and four dimensions of "budget and equipment, information resource development, human resource development and organization structure" was applied. In both questionnaires according to the number of questions for each component, grading was based on Likert scale from 1 to 5, and then the impact of each component on research ethics on the quality of research was assessed. FINDINGS: From 320 samples, 223 (69.7%) were male and 97 (30.3%) were female. The mean score of trustworthiness was 24.5±20.69 in males and 25.5±72.81 in females (p<0.05). Research ethics with standard coefficient (0.814) had an impact on research quality. The results also indicated that among the dimensions of research ethics, trustworthiness, responsibility, subject rights and teamwork spirit had a positive and significant effect on research quality (p <0.05). CONCLUSION: The results of this study demonstrated that research ethics has an impact on the quality of research of faculty members of Medical Sciences Universities in a large area of the country. 8229 Sport Physiology The Effect of Eight Weeks of Continuous Endurance Training on ICAM-1 and VCAM-1 Expression in the Heart Tissue of Rats Asad MR Sistani M Barzegari A 1. Department of Physical Education and Sport, Payame Noor University, Tehran, I.R.Iran 1. Department of Physical Education and Sport, Payame Noor University, Tehran, I.R.Iran 1. Department of Physical Education and Sport, Payame Noor University, Tehran, I.R.Iran 1 12 2019 21 1 230 236 11 10 2018 09 04 2019 BACKGROUND AND OBJECTIVE: The expression of adhesion molecules in the heart tissue is considered as an important indicator in the estimation of coronary artery disease risk. Since physical activity is effective in reducing symptoms of cardiac disease, the purpose of the present study was to investigate the effect of eight weeks of continuous endurance training on ICAM-1 and VCAM-1 expression in the heart tissue of rats. METHODS: In this experimental study, 12 eight weeks male Wistar rats with an average weight of 263±12 g were randomly divided into 2 groups: control (n=6) and exercise (n=6) were divided. The training program included 8 weeks of running on a treadmill with a 70 to 75% VO2max intensity (five days a week and 30 minutes each day). The expression of ICAM-1 and VCAM-1 genes were measured by Real-time PCR and compared in two groups. FINDINGS: The results showed that 8 weeks of continuous endurance training reduced the expression of ICAM-1 in the heart tissue. In comparison, there was a significant difference between the control group (0.29±0.05) and training (0.10±0.03) (p=0.017). In addition, it was shown that the expression of VCAM-1 in the heart tissue significantly increased in training group (0.85±0.04) compared to the control group (0.22±0.03) (p=0.001). CONCLUSION: The results of this study showed that continuous endurance training altered the expression of adhesion molecules in heart tissue associated with systemic inflammation, which could be a suitable solution for reducing fat deposits in the heart tissue and preventing its harm. 7813 Microbiology Evaluating Antibacterial Effect of Green Synthesis Oxide Iron Nanoparticles Using Cytoplasmic Extract of Lactobacillus casei Torabian P Ghandehari F Fatemi M 1. Department of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, I.R.Iran 1. Department of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, I.R.Iran 2. Department of Biology, Islamic Azad University, Falavarjan Branch, Isfahan, I.R.Iran 1 12 2019 21 1 237 241 01 05 2018 22 04 2019 BACKGROUND AND OBJECTIVE: With regard to the drug resistance, special attention has been placed on researches for discovering new antimicrobial substances such as nanoparticles. In green synthesis biological resources such as bacteria and yeast are used for producing metal nanoparticles.  In this research, we used the Lactobacillus casei extract as a biological source for producing iron oxide nanoparticles and antibacterial effects of these nanoparticles against the standard strains of Escherichia coli and Staphylococcus aureus were investigated. METHODS: for synthesis of green iron oxide nanoparticles, cytoplasmic extract of Lactobacillus casei and iron sulfate solution 10-3M were mixed and incubated for 3 weeks at 37 ° C in the presence of 5% carbon dioxide. Synthesis of iron oxide nanoparticles were evaluated using X-ray diffraction pattern and Transmission electron microscopy. Antibacterial effects of nanoparticles with dilution of 10, 100 and 1000 micro-grams per milliliter on two standard strains of Escherichia coli and Staphylococcus aureus were evaluated. FINDINGS: Synthesis of iron oxide crystals was confirmed by XRD analysis. Based on transmission electron microscopy the average of nanoparticles was about 15 nm with a spherical shape. Antimicrobial effect of nanoparticles showed that at concentration of 100 and 1000 µg/ml has an inhibitory effect on the growth on Staphylococcus aureus equal to 12.03±0.32 and 16±0.5, respectively. The only concentration that showed significant effect was 1000µg/ml (p≤0.001). While are ineffective against Escherichia coli. CONCLUSION: The results of this study indicated that producing iron oxide nanoparticles using cytoplasmic extract of Lactobacillus casei is a biologically safe method and very noteworthy in medicine and pharmacology and may be considered as a good candidate for the treatment of bacterial infections. 8140 Biochemical Cytotoxicity Effects of Green Synthesized Silver Nanoparticles by Using the Extract of Tuber Spp. on Breast Cancer (MCF-7) Cells Nedaie Sh Akbari H Yaghoubi H 1. Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, I.R.Iran 2. Department of Physics, Ardabil Branch, Islamic Azad University, Ardabil, I.R.Iran 1. Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, I.R.Iran 1 12 2019 21 1 242 248 05 09 2018 12 05 2019 BACKGROUND AND OBJECTIVE: Silver nanoparticles have antibacterial and anticancer activity. But the organic solvents used to produce these nanoparticles are toxic and can have devastating environmental effects. Therefore, there is a strong desire to use healthy methods for the synthesis of silver nanoparticles. Therefore, this study was performed to synthesize silver nanoparticles biologically from aqueous extract of Truffle and to study their biological effects on cancer cells. METHODS: In this experimental study, to synthesize silver nanoparticles from aqueous extract of Truffle, 10 ml of fungus extract was added to 90 ml of 1 ml silver nitrate solution and incubated for 72 hours at room temperature. The physicochemical properties of the nanoparticles synthesized by XRD, FESEM and TEM were analyzed. Effects of Extract and Synthesized Nanoparticles were studied by MTT at concentrations of 0.025, 0.25, 0.25, 0.5 and 1 mg / ml on MCF-7 Cancer Cells at 72, 48, and 24 hours. FINDINGS: The size of the nanoparticles was between 19 and 35 nm and their shape was mainly spherical. In cytotoxicity assay using MTT assay IC50 calculated for silver nanoparticles and extract in MCF-7 cells showed that silver nanoparticles had more cytotoxic effects than extract. The calculated IC50 for extracts and nanoparticles at 24, 48 and 72 hours were 0.73, 0.8, and 0.64 mg / ml, respectively, and for the nanoparticles were 0.6, 0.5, and 0.48, respectively (p <0.05). CONCLUSION: The silver nanoparticles produced by bio-synthesis method have a higher cytotoxicity than the truffle fungus (Tuber spp.) extract. 8176 Nutrition The Role of Melatonin on Metabolic Factors related to Periodontal Disease in Patients with Type 2 Diabetes Mellitus Bazyar H Alipour M Mirzaee F Moradi Poodeh B Zare Javid A 1. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R.Iran 2. Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R.Iran 1. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R.Iran 1. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R.Iran 3. Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R.Iran 1 12 2019 21 1 249 258 14 09 2018 10 04 2019 BACKGROUND AND OBJECTIVE: Regarding to various controlling and therapeutic interventions, the risk of periodontal disease in diabetics is three times that of non-diabetics. Because of the central role of oxidative stress in the pathogenesis of diabetes, interest in the use of antioxidants, including melatonin, as a complete therapeutic approach has increased. Therefore, this review study was performed to investigate the role of melatonin on metabolic factors associated with periodontal disease in patients with type 2 diabetes. METHODS: This review study was conducted on various databases including Scopus, PubMed, Science Direct, Google Scholar and Persian databases such as Magiran and SID and keywords such as type 2 diabetes, periodontal disease, melatonin, hyperglycemia, lipid profile, hypertension, obesity, and Inflammatory factors were carried out from 2000 to 2018. FINDINGS: A review of studies indicates that melatonin supplementation can reduce progressive damage of periodontal tissue, blood glucose levels, lipid profiles, hypertension, obesity and inflammatory factors in T2DM patients with periodontal disease, and therefore it has a significant role in improving of these patients. On the other hand, it has been shown that increased blood glucose can reduce the production of melatonin from the pineal gland in diabetic patients. Therefore, the supplementation with melatonin in these patients can play a useful role in increasing the production of melatonin in the body by reducing blood glucose levels. CONCLUSION:The obtained results showed that melatonin supplementation with its antioxidant role can have a beneficial role in improving the survival of T2DM patients with periodontal disease by balancing inflammatory and anti-inflammatory cytokines. 8387 Physiotherapy Effects of Lateral Wedge Insole on Static and Dynamic Balance in Patients with Moderate Medial Knee Osteoarthritis Sayadnejad F Esfandiarpour F Rezazadeh A Amin M Derisfard F Shaterzadeh MJ 1. Musculoskeletal Rehabilitation Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R.Iran 2. Department of Family Medicine, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Canada 3. Department of Radiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R.Iran 1. Musculoskeletal Rehabilitation Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R.Iran 1. Musculoskeletal Rehabilitation Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R.Iran 1. Musculoskeletal Rehabilitation Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I.R.Iran 1 12 2019 21 1 259 265 18 12 2018 13 08 2019 BACKGROUND AND OBJECTIVE: Knee osteoarthritis (OA) is associated with diminished joint proprioception, balance deficits and increased risk of falls. Patients with medial knee osteoarthritis are at higher risk of balance deficits and falling due to the development of secondary varus deformity. Therefore, it is of great clinical importance to identify the treatment methods that could improve balance in knee OA. The purpose of this study was to evaluate the effects of Lateral Wedge Insole (LWI) on static and dynamic balance in patients with moderate medial knee osteoarthritis. METHODS: Thirty patients with moderate medial knee OA participated in this semi-experimental interventional study. Patients were recruited from orthopedic and rheumatology clinics of the university. For each patient, the static and dynamic balance were assessed in two conditions including wearing shoes with flat insole and wearing shoes with lateral wedge insole, using a Prokin stabilometer. The balance variables were the anteroposterior and mediolateral velocity and standard deviation of the center of pressure (COP) displacement, and total stability indices. Patients performed two familiarization trials, then, three actual trials with at least 30 s interval for each test. FINDINGS: Wearing LWIs resulted in significant decreases in the anteroposterior (static:1.78mm/s, dynamic:6.63mm/s) and mediolateral velocity of COP (static:1.53mm/s, dynamic:5.58mm/s) in compression to wearing shoes with flat insole (p<0.001). The anteroposterior (static:1.04 mm, dynamic:1.57mm) and mediolateral SD of the COP (static:1.58mm, dynamic:2.02mm) were also decreased after using LWIs (p<0.007). Except for the anteroposterior stability index, other balance indices were also significantly improved (p<0.05). CONCLUSION: Our findings suggest that using a LWI improve static and dynamic balance in patients with moderate medial knee OA. 8426 Histology Neural Differentiation of Wharton's Jelly Stem Cells in Three-Dimensional Chitosan Based Culture Environment Mansouri E Kermani Sh Alizadeh A 1. Department of Material Engineerig, Najafabad Branch, Islamic Azad University, Najafabad, I.R.Iran 1. Department of Material Engineerig, Najafabad Branch, Islamic Azad University, Najafabad, I.R.Iran 2. Department of Tissue Engineering and Applied Cell Sciences, School of Medicine, Semnan University of Medical Sciences, Semnan, I.R.Iran 1 12 2019 21 1 266 272 01 01 2019 01 07 2019 BACKGROUND AND OBJECTIVE: Wharton Jelly Stem Cells (WJSCs) can be a good option for differentiating and regeneration of nervous system damage. Three dimensional (3D) cell cultures by providing a body-like environment have more advantages than the two dimensional (2D) cell cultures. The aim of this study was to investigate the neural differentiation of these cells in a 3D chitosan based culture environment. METHODS: This experimental study was performed in 4 groups of 2&3D with and without differentiation media on WJSCs. First, to construct the hydrogel, hydroxyl ethyl cellulose was added to chitosan-beta-glycerophosphate solution (8.4: 0.8) (HEC: CH-β-GF). Human WJSCs after isolation by enzymatic method from wartons' jelly of born infant in  Imam Khomeini hospital in Tehran and characterization with flow cytometry, were cultured 5Í10 5 cell in each well of 24-well plate in a 2D and 3D environment using the hydrogel in neural differentiation media for 4 days. Then, the neural differentiation of WJSCs was evaluated by quantitative analysis of β-Tubulin III, Nestin and β-actin (internal control) genes expression by Real Time PCR (RT-PCR). FINDINGS: The results of RT-PCR showed that expression of β-Tubulin III and Nestin genes in WJSCs was significantly increased by the influence of the neural differentiation media in both 2D (more than 4 folds)and 3D (more than 2 folds) culture conditions (p <0.005). But the expression of β-Tubulin III and Nestin in 3D cell culture condition (more than 1.5 folds) was greater than that in the 2D cell culture condition under the influence of the neural differentiation media (p <0.01). CONCLUSION: The results showed that neural differentiation of WJSCs in a chitosan based 3D environment is higher than 2D. 8246 Genetics, Cell and Molecular Biology Effect of Omega-3 on Rat Sperm DNA Methylation and Histological Structure of Testis after Treatment with Bleomycin, Etoposide and Cisplatin (BEP) Razavi Sh Hashemi FS Khadivi F 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R.Iran 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R.Iran 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R.Iran 1 12 2019 21 1 273 278 15 10 2018 25 05 2019 BACKGROUND AND OBJECTIVE: During the cancer treatment course, in addition to the destructive effects on the tumor cells, chemotherapy also damages healthy tissues and disrupts the balance of oxidant and antioxidant levels. The present study was conducted to evaluate the effect of omega-3 on sperm DNA methylation and histological structure of rat testis after treatment with combination chemotherapy using bleomycin, etoposide and cisplatin (BEP). METHODS: In this experimental study, 40 male rats were randomly divided into four groups of control, BEP, BEP+omega-3 and omega-3 (n=10). The control group was treated with 0.9% normal saline intraperitoneally for 18 weeks. The second group (BEP) first received 0.9% normal saline intraperitoneally for nine weeks. Then, it received BEP at 5.1 mg / kg for nine weeks, received etoposide and cisplatin at 5.7 mg/kg through gavage on days 1-5 of each week, and then received bleomycin at 75 mg/kg on days 2 of each week. The third group was gavaged with 0.9% saline for 9 weeks and then, orally received 300 mg/kg/day omega-3(capsule containing 1000 mg, 18% EPA and 12% DHA) for 9 weeks and in BEP + omega-3 group treated with BEP based on the same method and then orally received 300 mg/kg omega-3 as an antioxidant for the second nine weeks daily. Sperm DNA methylation and histological structure of rat testis including seminiferous tubules and basement membrane thickness were respectively evaluated by immunofluorescence staining and Periodic acid – Schiff (PAS) after 18 weeks of treatment in all groups.   FINDINGS: The mean percentage of sperm DNA methylation in the BEP-treated group (52.22±3.11) was significantly decreased compared to the control group (81.80±2.92) (p<0.001). However, the mean percentage of sperm DNA methylation increased significantly with omega-3 use after treatment with BEP (67±2.18) compared with BEP group (p<0.01). In light microscopy of testicular tissue, the number of spermatogonial cells (44.95±1.56), primary spermatocytes (47.60±1.45) as well as the epithelial thickness of seminiferous tubules (145.5±5.64) and basement membrane (7.07±0.29) decreased in the BEP-treated group in comparison with control group (p<0.001). However, the use of omega-3 after treatment with BEP significantly improved the number of germ cells and epithelial thickness of the seminiferous tubule and basement membrane (p<0.01). CONCLUSION: Based on the results of this study, omega-3 as an antioxidant can improve the cytotoxic effects of chemotherapy drugs and it is recommended to be used for cancer patients after chemotherapy to reduce the cytotoxicity of these drugs. 8172 Medical Physics Evaluation of Physical Parameters of Skin by Consecutive Ultrasonic Image Processing During Ultraviolet Radiation in an Animal Model of Wrinkled Skin Estaji M Mokhtari M Movahedin M Ghaffarri S 1. Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R.Iran 1. Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R.Iran 2. Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R.Iran 3. Department of Pathology, Faculty of Veterinary Medicine, Semnan University, Semnan, I.R.Iran 1 12 2019 21 1 279 285 12 09 2018 10 04 2019 BACKGROUND AND OBJECTIVE: Skin aging is divided into two categories of intrinsic and extrinsic aging. Skin aging due to repeated exposure to ultraviolet radiation (extrinsic aging) is different from aging caused by time (intrinsic aging). The appearance of wrinkles caused by sunlight is due to subcutaneous fat atrophy and reduced production of collagen and elastin, thereby altering the biomechanical properties of the skin tissue. This study was conducted to investigate the skin damage caused by ultraviolet B (UVB) radiation by consecutive ultrasonic image processing with high resolution. METHODS: In this experimental study, we evaluated the skin injury process among 25 C57BL/6 mice in healthy group (zero dose), and case group exposed to UVB radiation at 0.03 milliwatts per square centimeter (5 times a week for 5 weeks) due to differences in skin characteristics. Physical parameters of dermal and epidermal layers were measured and evaluated weekly from day 7 to day 35 using ultrasonic image processing. FINDINGS: The thickness of the dermal and epidermal layers obtained by ultrasonic processing during the process of ultraviolet radiation injury in the mouse model significantly increased during the 5 – week study (p < 0.05). In addition, the percentage of changes in the thickness of the epidermis layer (from 0.22±0.01 mm on day zero to 0.37 ± 0.02 mm on the thirty-fifth day) and the dermal layer (from 0.57 ± 0.05 on day zero to 0.90 ± 0.08 mm on the thirty-fifth day) showed 68% and 57% increase, respectively. CONCLUSION: The results showed that UVB radiation increased the thickness of the skin layers.   8484 Microbiology Detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride Hadadi F Ghaznavirad E Almasi-Hashiani A Abtahi H 1. Students Research Committee, Arak University of Medical Sciences, Arak, I.R.Iran 2. Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, I.R.Iran 3. Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, I.R.Iran 2. Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, I.R.Iran 1 12 2019 21 1 286 292 26 01 2019 01 07 2019 BACKGROUND AND OBJECTIVE: The resistance genes of quaternary ammonium compounds(qac) play an important role in the resistance of gram-negative bacteria producing broad-spectrum beta-lactamases to disinfectants. The aim of this study was detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride. METHODS: This study cross sectional-descriptive was conducted on 150 clinical samples of selected hospitals in Arak. ESBL strains were identified by using phenotypic methods of disc diffusion and combinatory disc method and evaluating the SHV, TEM, CTXM1 genes by genotyping method. The PCR was performed to determine the resistance genes qacEΔ1, qacG, qacE and qacF.The electrophoresis of PCR products and the MIC of benzalkonium chloride were relative to E. coli producing ESBL. Antibiotic pattern of Escherichia coli (ESBL), quadruple ammonium resistance genes and benzalkonium chloride MIC were also investigated. FINDINGS: This study showed that 60% of Escherichia coli were ESBL producer. The qacEΔ1 genes were observed in all of them and qacE, qacF, qacG genes were not found in any of the strains. The strains had MIC range from 32 to 64 mg/l for benzalkonium chloride. Resistance to carbapenems (33.33%) was observed. CONCLUSION: This study showed that qacEΔ1 resistance gene and resistance to disinfectant benzalkonium chloride increased. Also increased resistance to the antibiotics studied were observed in E. coli ESBL strains. 8410 Health Food Comparison of the Effects of Hydrosol Extracted from Turmeric (Curcuma longa) and Cinnamon (Cinnamomum verum) on staphylococcal biofilm Didar Z Mohamadisani A 1. Department of Food Science, Neyshabur Branch, Islamic Azad University, Neyshabur, I.R.Iran 2. Department of Food Science, Quchan Branch, Islamic Azad University, Quchan, I.R.Iran 1 12 2019 21 1 293 298 27 12 2018 24 08 2019 BACKGROUND AND OBJECTIVE: The biofilm of bacteria, especially pathogenic bacteria, endanger food safety due to their high resistance to disinfectants; use of herbal extracts, essential oils and hydrosol during extraction of essential oils is one of the ways to combat the resistance microbial biofilms. Therefore, the effect of hydrosol extracted from turmeric (Curcuma longa) and cinnamon (Cinnamomum verum) on staphylococcal biofilm was investigated in this study. METHODS: In this experimental study, the hydrosol of turmeric and cinnamon was extracted using steam distillation method. After isolation of essential oil, 10, 30 and 50% concentrations were used to investigate the anti-biofilm effects. Anti-biofilm effect against Staphylococcus aureus (PTCC 1112), Staphylococcus saprophyticus (PTCC 1440) and Staphylococcus epidermidis (PTCC 1435) was evaluated using ELISA reader. The efficacy of both hydrosols in removing bacterial biofilms formed on different surfaces (glass, steel and polyvinyl chloride) was determined by microbiological culture method. FINDINGS: The results showed that optical density in control samples of Staphylococcus aureus, epidermidis and saprophyticus was 0.254±0.03, 0.138±0.019, and 0.146±0.017, respectively, but after exposure to 50% cinnamon hydrosol reached 0.072±0.011, 0.096±0.021, and 0.064±0.01, and after exposure to 50% turmeric hydrosol reached 0.074±0.02, 0.098±0.021, and 0.057±0.011, respectively. Investigation of the efficiency of hydrosol in removal of formed biofilms showed that the highest decrease was in the case of Staphylococcus saprophyticus biofilm formed on glass and steel surfaces, and the logarithm of bacterial population declined from 4 to 1 in the presence of 50% hydrosol (p ≤ 0.05). CONCLUSION: The results of this study showed that the hydrosol extracted from cinnamon and turmeric is effective in preventing biofilm formation of staphylococcal bacteria and eliminating the formed biofilm.   8253 Emergency Medicine Comparison of Triage Effectiveness Based on ESI and HEART Scale in Diagnosis of Outcome in Patients with Chest Pain Gharaee R Zohari Anboohi S Shiri H Nasiri M 1. Student Research Committee, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 2. Department of Medical Surgical Nursing, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 2. Department of Medical Surgical Nursing, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 3. School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 1 12 2019 21 1 299 305 18 10 2018 04 08 2019 BACKGROUND AND OBJECTIVE: Chest pain is one of the common causes of emergency department visit, but only 25-15% of them are diagnosed with acute coronary syndrome (ACS). However, unexplained cases of ACS have led to high mortality rates. The aim of this study was to compare the effectiveness of ESI triage (Emergency Severity Index) and HEART scale (Chest Pain Diagnostic Scale) in detecting the outcome of patients with complaints of chest pain. METHODS: This descriptive study was performed on 200 patients with chest pain in emergency department of selected hospitals of Shahid Beheshti University of Medical Sciences in 2017. The ESI triage form, HEART and demographic information were completed for all patients. Six weeks later patients were re-evaluated in terms of heart problems. FINDINGS: The compliance of the ESI triage and HEART scale in the high-risk group was 27.9% (24 people), the middle-risk group was 79% (62 people) and the low-risk group (30.6%) (11 people). In general, comparing the total scores, these two scales were consistent with 48%. Also, after considering the cut-off point 4.5, the sensitivity 85% and the specificity 89% with a 95% confidence interval for the HEART scale and sensitivity 95%, and the specificity 25% with a 95% confidence interval was found for the ESI triage. CONCLUSION: According to the results of this study, despite its wide range, the ESI triage has shown a poor performance over the HEART scale. Therefore, the use of the combined HEART triage ESI scale can be very helpful. 8189 Nursing Comparison of the Effect of Peer-led Education and Education by the Healthcare Personnel on Awareness, Attitude and Performance of Lettered People toward Organ Donation Mohammadpour M Mohammadpour A Ajam zibad H Najafi S 1. Student Research Committee, School of Nursing, Gonabad University of Medical Sciences, Gonabad, I.R.Iran 2. School of Nursing, Gonabad University of Medical Sciences, Gonabad, I.R.Iran 3. Social Development & Health Promotion Research Center, School of Nursing, Gonabad University of Medical Sciences, Gonabad, I.R.Iran 3. Social Development & Health Promotion Research Center, School of Nursing, Gonabad University of Medical Sciences, Gonabad, I.R.Iran 1 12 2019 21 1 306 313 23 09 2018 09 04 2019 BACKGROUND AND OBJECTIVE: Lack of donation is one of the major limitations of organ transplantation. Promoting organ donation requires increased awareness and creation of a positive attitude through training in specific ways. The purpose of this study was to compare the effect of peer-led education and education by healthcare personnel on awareness, attitude and performance of lettered people in regard with organ donation. METHODS: This case-control clinical trial was performed on 120 lettered people who were randomly assigned to three groups of 40, including peer-led, healthcare personnel and control groups. The two intervention groups underwent training sessions. Before and after the intervention, the data were collected and compared using questionnaires in three groups in three areas of awareness, attitude and performance. FINDINGS: Most of the lettered people (58.33%) were female. 61.66% had a bachelor's degree and 60.83% were teachers at elementary school. There was a significant difference in the mean scores of awareness, attitude and performance toward organ donation in the two intervention groups before the intervention (in peer-led group were 11.52 ± 2.12, 92.05 ± 10.24, and 1.87 ± 1.42 and in healthcare group were 11.30 ± 3.00, 90.73 ± 10.92, and 1.38 ± 1.29, respectively) and after the intervention (in peer-led group were 15.47 ± 1.13, 98.78 ± 5.70, and 3.53 ± 0.50 and in healthcare group were 15.13 ± 1.49, 96.95 ± 5.52, and 3.05 ± 0.81, respectively) ( P < 0.05). The mean score of attitude and performance of the lettered people in the peer-led group was significantly higher than the other groups (P < 0.05).  CONCLUSION: The results of the study showed that peer-led education is a more effective method than education by healthcare personnel to promote and institutionalize organ donation. 7989 Psychiatry The Epidemiology of Psychiatric Disorders in Children and Adolescents in Mazandaran Province Nasiri M mohammadi MR Ahmadi N Alavi SS Rezazade H Ostovar rostami F Naderi H Sanaee moghadam F Rahimi pashakolaei Z Barzegar damadi MA Amiri talar poshti R Hosseini SH 1. Alzahra University, Tehran, I.R.Iran. 2. Psychiatry and Psychology Research Center, Roozbeh Hospital, Tehran University of Medical Sciences, Tehran, I.R.Iran 3. Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, I.R.Iran 2. Psychiatry and Psychology Research Center, Roozbeh Hospital, Tehran University of Medical Sciences, Tehran, I.R.Iran 4. Allameh Tabatabei University, Tehran, I.R.Iran 5. Islamic Azad University, Sari Branch, Sari, I.R.Iran 6. Shahid Beheshti University, Tehran, I.R.Iran 5. Islamic Azad University, Sari Branch, Sari, I.R.Iran 5. Islamic Azad University, Sari Branch, Sari, I.R.Iran 5. Islamic Azad University, Sari Branch, Sari, I.R.Iran 7. Islamic Azad University, Roudehen Branch, I.R.Iran 8. Psychiatry and Behavioral Sciences Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, I.R.Iran 1 12 2019 21 1 314 319 08 07 2018 09 04 2019 BACKGROUND AND OBJECTIVE: New information on the epidemiology of psychiatric disorders in children and adolescents is essential for planning and management of health system. Despite the significance of recognizing the health status of children and adolescents, the prevalence of psychiatric disorders in children and adolescents in Iran has not been systematically investigated. This study, which is part of the National Survey of Iranian Children and Adolescents (IRCAP), examines the prevalence of psychiatric disorders in children and adolescents in Mazandaran province, northern Iran. METHODS: In a cross-sectional survey, 1051 children and adolescents residing in Mazandaran, aged 6 – 18 years, were selected by multistage cluster random sampling. In addition to demographic data, the Persian version of K-SADS-PL (Schedule for Affective Disorders and Schizophrenia for School-Age Children--Present and Lifetime Version) was used to assess psychiatric disorders based on Axis I of the DSM-IV. FINDINGS: The overall prevalence of psychiatric disorders is 18% (20.42 – 15.78), mood disorders is 2% (3.15 – 1.38), psychotic disorders is 0.1% (0.2 – 55.02), anxiety disorders is 10.4% (12.8 – 36.67), separation anxiety disorder is 4.6% (6.3 – 01.46), post-traumatic stress disorder (PTSD) is 0.3% (0.8 – 0.1), behavioral disorders is 5.1% (6.3 – 65.96), attention deficit hyperactivity disorder (ADHD) is 2.9% (4.16 – 2.09), conduct disorder is 2.0% (0.0 – 8.1), neurodevelopmental disorders is 2.1% (3.1 – 15.38) and mental retardation is 0.8% (1.4 – 49.0). 42.9% of the cases with depressive disorders also have anxiety disorders. 17.4% of anxiety disorders are associated with behavioral disorders. CONCLUSION: According to the results of this study, psychiatric disorders are considerably common among children and adolescents in Mazandaran. Anxiety disorders are the most common and psychotic disorders are the least common group of psychiatric disorders. Separation anxiety disorder is the most common and post-traumatic stress disorder (PTSD) is the least common anxiety disorder. Attention deficit hyperactivity disorder (ADHD) is the most common and conduct disorder the least common behavioral disorder. Among psychiatric disorders, the highest comorbidity is between depression and anxiety disorders. 8294 Traditional Medicine Cardiotonic Medicines (Mofarrehs) and Their Mechanism of Action in Persian Medicine Kamaneh SAR Mojahedi M Mozafari O Memariani Z Saravani M 1. Health Management and Social Development Research Center, Golestan University of Medical Sciences, Gorgan, I.R.Iran 2. Traditional Medicine and History of Medical Sciences Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 1. Health Management and Social Development Research Center, Golestan University of Medical Sciences, Gorgan, I.R.Iran 3. Department of Persian Medicine, School of Persian Medicine, Babol University of medical sciences, Babol, I.R.Iran‎ 1. Health Management and Social Development Research Center, Golestan University of Medical Sciences, Gorgan, I.R.Iran 1 12 2019 21 1 320 330 07 11 2018 05 08 2019 BACKGROUND AND OBJECTIVE: According to Persian Medicine (PM), heart is one of the main organs that maintain the human health. This medical school has introduced several medicines to maintain the health of the heart or to treat its diseases, such as cardiotonic medicines (Mofarrehs). In this study, Mofarrehs and their function are described from the perspective of PM. METHODS: In this simple review article, six books from the main sources of PM, including Al-Shamel, Kitab al-Adviyt ol Qalbiye, Qarabadin-e-Kabir, Advieh Ghalbieh, Canon of Medicine and Kholaseh-al-Hekamah were reviewed and Mofarrehs and their function were analyzed. Qualitative content analysis was also performed, and then articles related to the mechanism of action of these drugs were extracted and the name of each Mofarrehs was searched in PubMed, Google Scholar, Scopus, SID, and IranMedex databases. FINDINGS: Approximately, 30 commonly used Mofarrehs were identified and their characteristics including name, Persian and scientific names, temperament (Mizaj), origin and mode of effect on the heart were concluded and given as follows. In Persian medicine, some foods have been also regarded as Mofarrehs, and in this review, we discuss the mechanisms through which the tonic effects are exerted. Its antidepressant effects due to its antioxidant activity and inhibition of free radical has been proven in recent studies. CONCLUSION: Administration of Mofarrehs, could initiate the future clinical trials to develop a new and effective natural drugs in the treatment of cardiac and mental illnesses. However, its wide acceptance needs further investigation.   8596 Biochemical The Effect of Hydroalcoholic Extract of Glaucium Flavum on the Activity of Anti Oxidative Enzymes in the Heart and Brain of Alloxan Induced Diabetic Rats Khoshvaghti A Darya G H Hashemi F Klantari M Hushmandi K 1.Department of Clinical Sciences, Faculty of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, I.R.Iran 2.Department of Comparative Biomedical Science, Faculty of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, I.R.Iran 3.Young Researchers and Elite Club, Kazerun Branch, Islamic Azad University, Kazerun, I.R.Iran 4.Department of Genetic Science, Faculty of Advanced Sciences and Technology, Medical science Branch, Islamic Azad University, Tehran, I.R.Iran 5.Department of Epidemiology and Zoonosis, Faculty of Veterinary Medicine, Tehran University, Tehran, I.R.Iran 1 12 2019 21 1 331 337 06 03 2019 21 09 2019 BACKGROUND AND OBJECTIVE: Oxidative stress is the connection between diabetes and neuropathies and micro vascular disorders. This study was designed to investigate the effect of hydroalcoholic extract of Glaucium Flavum on the tissue activity of antioxidant enzymes in the heart and brain of Alloxan induced diabetic rats. METHODS: In this experimental study, 32 male rats were randomly divided into four groups of eight including control, diabetic, diabetic rats treated by Glaucium Flavum with dose of 500mg/kg and diabetic rats treated with Glibenclamide 5μg/kg. Diabetes was induced by single injection of 120mg/kg of Alloxan. After one month, activity of SOD, CAT and GPX were measured in the heart and brain tissues and analyzed. FINDINGS: Cardiac activity of all three enzymes in the diabetic + extract group were significantly higher than diabetic control (p<0.001). Activity of SOD in brain had a significant difference in comparison to diabetic + extract (8.79±1.4) and diabetic +drug groups (6.77±1.7) (p=0.03). As the same, CAT activity in diabetic+extract group (4.64±1.2) was significantly higher than diabetic + drug group (3.83±1.5) (p<0.001). Similar to the two previous state, GPX activity in diabetic+extract group (4.23±0.7) was significantly higher than diabetic+drug group (3.64±0.4) (p=0.03). CONCLUSION: The present study declared that yellow Glaucium Flavum extract can promote the main protective enzymatic mechanisms against diabetic induced oxidative stress in heart and brain. In addition, the effect of the extract was more successful than the effect of Glibenclamide and this effect was more pronounced in brain tissue. 8359 Descriptive Sciences The Effect of Gender on Dimensions of Lumbar Vertebral Pedicle Using Computed Tomography Momeni-e-Raqla S Heidari K Gharib M H Mohammadi M R Golalipour M J 1. Student Research Committee, Golestan University of Medical Sciences, Gorgan, I.R.Iran 2. Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, I.R.Iran 3. Department of Radiology, Golestan University of Medical Sciences, Gorgan, I.R.Iran 4. Department of Neurosurgery, Golestan University of Medical Sciences, Gorgan, I.R.Iran 5. Gorgan Congenital Malformations Research Center, Golestan University of Medical Science, Gorgan, I.R.Iran 1 12 2019 21 1 338 344 08 12 2018 15 09 2019 BACKGROUND AND OBJECTIVE: Having the correct anatomical points and the size of the pedicle dimension is necessary to minimize neurological complications. Currently, the gold standard for spinal fusion is the use of pedicle screws. In this method, the pedicle screw should be placed in its ideal position. Given the importance of this topic, the present study was performed to determine the dimensions of right and left pedicle by computed tomography (CT) scan in three axial, coronal and sagittal planes based on gender in Fars-native ethnic group. METHODS: This cross-sectional study was performed on 40 patients (20 females and 20 males aged 18 – 45 years) from Fars-native ethnic group. The average height for males was 165 to 180 cm and for females between 165 and 175 cm. Healthy vertebrae were selected in people who referred to a CT scan unit with symptoms of spinal pain or trauma. Determination of height, length and width of right and left pedicle of lumbar vertebrae in axial, coronal and sagittal view in 1.5 mm sections was performed by CT scan. FINDINGS: Mean and standard deviation of height (1.458±0.141), width (0.788±0.173), and length (1.248±0.195) of pedicle in men (based on millimeter) was significantly higher compared to mean and standard deviation of height (1.268±0.140), width (0.618±0.148), and length (1.08±0.174) in women (p<0.05). CONCLUSION: The results of this study showed that gender affects the dimensions of lumbar vertebral pedicle. 7869 Obstetrics and Gynecology Successful Pregnancy and Delivery after Uterine Rupture in Previous Pregnancy: A Case Report Bouzari Z Mohammadnataj M Ghanbarpour A 1. Cellular & Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 2. Georgetown University, Washington, D.C, USA 3. Clinical Research Development Unit of Rouhani Hospital, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 1 12 2019 21 1 345 348 21 05 2018 08 01 2019 BACKGROUND AND OBJECTIVE: Uterine rupture in all layers is not only a serious complication of pregnancy but is also associated with maternal and fetal mortality. The usual treatment for uterine rupture is termination of pregnancy, and hysterectomy is necessary in most cases. Successful repair of uterine rupture in all layers is not only uncommon but successful subsequent pregnancy is quite rare. A successful pregnancy is reported in a woman with a history of complete uterine rupture due to intramural pregnancy. CASE REPORT: A 28 – year – old pregnant woman, gravida 4, with a history of two abortions, and one delivery (stillbirth) was admitted to the high-risk pregnancy unit of Ayatollah Rouhani Hospital (Babol, Iran). She had a history of rupture of all layers of the uterus following intramural pregnancy in her third pregnancy, and at that time, she had undergone laparotomy and repair of the uterus. In the fourth pregnancy, due to the history of uterine rupture, she was under prenatal care in high-risk pregnancy unit, and after the onset of contractions at 36 weeks and 2 days, emergency cesarean section was performed for the patient, and the neonate was born with 10/10 APGAR score and a weight of 3000 grams. CONCLUSION: In women who become pregnant after a history of rupture of all layers of the uterus and after the repair of uterine, prenatal and fetal care should be done carefully and cesarean section should be performed immediately after the onset of labor contractions. 8774 Skin Effect of Visceral Fat on the Amount of Hydroxyproline and Histopathological Changes on Surgical Wound Healing in Male Rat Didevar K Davoodi S H Javdan Gh 1. Department of Biochemistry, Alborz University of Medical Sciences, Karaj, I.R.Iran 2. Minimally Invasive Surgery Research Center, Iran University of Medical Sciences, Tehran, I.R.Iran 3. Cancer Research Center, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran 1 12 2019 21 1 349 356 04 06 2019 18 09 2019 BACKGROUND AND OBJECTIVE: expensive treatments and rehabilitation has increased the tendency to use natural and cheaper compounds. Animal visceral lipids contain a high percentage of different types of effective anti-inflammatory fatty acids, which till now have not been studied together. Therefore, the purpose of this study was to evaluate the anti-inflammatory and regenerative effects of sheep visceral lipids on surgical wound healing in rat rats. METHODS: In this experimental study 50 Sprague-Dawley adult male rats with the average weight of 250-300 g were selected and randomly divided into 2 groups with 25 rats in each group. After providing visceral fat from the pre renal part of the young sheep and melting and sterilizing them, the treatment group which were wounded under surgical anesthesia by excisional wounds, were treated by 10 ml of it on the wound each day. The evaluation of the wound size was measured by using an accurate caliper and Image J software on days 5, 10, 12, 15. Tissue biopsies from both control and treatment groups were performed to compare the rate of epithelialization, collagen filament formation, inflammatory cells, vascularization, and fibroplasia in situ. Also a biopsy was performed at day 21th to check the amount of hydroxyproline in the tissue. FINDINGS: Based on morphometric findings, on the 10th day of treatment, the mean ulcer size of the treatment group with visceral fat was 43.6±0.44 mm2 while the mean size of the ulcer in control group was 98.5±0.07 mm2, which has shown to have the least amount of wound contraction. Also on the 5th day, the wound contraction of the treatment group, was twice as high as the control group(p<0.05). The histological examination shows the mean 2.74±0.17 points of restoration parameters score in treatment group compared to the control group with a score of 1.42±0.16 points. Also, the difference between the Hydroxyproline concentration of the healthy skin and the scarring wounds in treatment group (3.69±0.23 mg/g) was less than the control group(6.08±0.32 mg/g) (p<0.05). CONCLUSION: According to the findings of this study, the usage of animal visceral lipid, with anti-inflammatory effects and as a stimulator of collagen production, increases the amount of hydroxyproline and affects the healing process of cutaneous ulcers. It also improves the blood perfusion and angiogenesis by increasing RBC membrane flexibility, which will also enhance the migration of cells that are effective in wound healing. 7932 Nutrition Comparison of Anthropometric and Conicity indicators in Students with Premenstrual Syndrome Ramezani A Hoseini SM Chaleshgar Kordasiabi M 1. Diabetes Research Center, Mazandaran University of Medical Sciences, Sari, I.R.Iran 2. Department of Biostatistics, Mazandaran University of Medical Sciences, Sari, I.R.Iran 3. Health Sciences Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, I.R.Iran 1 12 2019 21 1 357 363 10 06 2018 23 01 2019 BACKGROUND AND OBJECTIVE: Body composition and body fat are important in the prevention of chronic diseases in people with premenstrual syndrome. This study was performed to compare the anthropometric and conicity indexes in PMS and healthy people. METHODS: This cross-sectional study was performed on 92 dormitory students living in Mazandaran University of Medical Sciences over 18 years of age in two groups with premenstrual syndrome and healthy subjects. Anthropometric and conicity parameters were measured and compared in two groups. FINDINGS: In this study, 46 (54.8%) patients in the PMS group with an average age of 22.02±1.51 years and 38 (45.2%) in the healthy group with an average age of 21.34±2.32 that there was no significant difference between the two groups in the test (p=0.08). In the PMS group, this disorder was significantly different from that of the healthy group (p=0.0001), and the anger and allergies and anthropometric indices were significantly higher in students with menstrual syndrome than in healthy subjects (see The order of p=0.003, p=0.007, p=0.001, p= 0.02, p= 0.01, p= 0.001), but the two groups did not have a significant difference in terms of conicity. CONCLUSION: The results of this study showed that subjects with premenstrual syndrome group had higher anthropometric indices than healthy subjects. But they do not differ in terms of conicity.   7794 Nursing The Effectiveness of Training Program Based on Virtual Participation on Stress and Anxiety of Parents with Premature Infants Hospitalized in Neonatal Intensive Care Units Zahedpasha Y Arzani A Khafri S Darvish A Esmaeili S 1. Non-Communicable Pediatric Disease Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 2. Nursing Care Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 3. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 4. Department of Medical_Surgical Nursing & Basic Sciences, School of Nursing and Midwifery, Tehran University of Medical Sciences, Tehran, I.R.Iran 5. Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran 1 12 2019 21 1 364 371 25 04 2018 13 11 2019 BACKGROUND AND OBJECTIVE: Due to lack of physical, emotional and psychological preparation, premature birth may cause a lot of stress for parents and may have consequences for both parents and infants. Since some parents have difficulty participating in face-to-face training programs, this study was conducted to determine the effectiveness of a training program based on virtual participation on stress and anxiety of parents with premature infants METHODS: This quasi-experimental study was performed among 80 parents of premature infants admitted to Amirkola Children's Hospital and Ayatollah Rouhani Hospital, who were randomly assigned to intervention and control groups with 40 participants in each group. The parents of the intervention group received the content of the virtual participation training program asynchronously in the form of Telegram groups within four days. The control group received no intervention other than the usual methods and support of the ward. Then, the State-Trait Anxiety Inventory (score 40-160) and the Parental Stress Scale (score 31-155) were completed and compared on the third and seventh days for both groups and on 13th day for the intervention group. FINDINGS: Results showed that parental stress score decreased from (97.118±21.68) to (86.27±00.27) (p<0.001) in the intervention group. In the control group, it changed from (76.103±30.44) to (91.99±31.82), which was not significant. In investigation of the effect of time on parental stress subscales, the mean scores of the questionnaires showed a significant difference from day 3 to day 13 in regard with the components of stressful parental experiences, relationship with the infant and parental role, parental experiences about behavior, appearance and treatment of the infant, and environmental stress (p<0.001). The parental state anxiety score in the intervention group changed from (45.68±4.63) to (46.63±3.54) and in the control group from (46.25±6.38) to (46.83±5.21), and the parental trait anxiety score in the intervention group changed from (43.88±5.91) to (43.97±5.59) and in the control group from (43.18±5.62) to (42.17±6.63), which was not significant. CONCLUSION: The results of the study showed that training program based on virtual participation reduces the stress of parents with premature infants.   8454 Genetics, Cell and Molecular Biology The Effects of Ibuprofen Cytoxic Dose on caspase-3, -8 and -9 Activity level in cervical cancer (Hela) cells Ghadiri E Ahmadi R Rostami E 1. Department of Biology, Faculty of Basic Sciences, Garmsar Branch, Islamic Azad University, Hamedan, I.R.Iran 2. Department of Physiology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, I.R.Iran 3. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, I.R.Iran 1 12 2019 21 1 372 377 12 01 2019 25 09 2019 BACKGROUND AND OBJECTIVE: Studies have shown that ibuprofen can have an anti-cancer effect on cervical cells, although the mechanism of this effect is not well known in cellular and molecular terms. Accordingly, the aim of this study was to investigate the effect of cytotoxic concentration of ibuprofen on the activity of caspases -3, -8 and -9 in cervical cancer (Hela) cells. METHODS: In this experimental-laboratory study, Hela cells were prepared from Pasteur Institute cell Bank and were divided into the control group and groups exposed to 0.01, 0.1, 1 and 10 mg/ml of Ibuprofen. Viability of cells was measured by MTT assay. The activity level of caspases-3, -8 and -9 was assessed by colorimetric method. The data were statistically analyzed using ANOVA. FINDINGS: The viability decreased significantly in cervical cancer cells exposed to 0.1 (76%), 1(64%) and 10(15%) mg/ml of ibuprofen compared to control group (100%)(p<0.05, p<0.001 and p<0.001 respectively). The caspases-3، -8 and -9 activity level increased significantly in cervical cancer cells exposed to IC50 dose of ibuprofen compared with control group (p<0.001, p<0.001 and p<0.01 respectively). CONCLUSION: The results of present study showed that ibuprofen is able to reduce the viability of cervical cancer cells in a dose-dependent pathway, and this pathway is induced by activating of caspases-3, -8 and -9.   8656 Neurology A Case Report of Tuberculosis with Extrapulmonary Manifestations Gazeran S Emami M Zemorshidi F 1. Department of Neurology, Faculty of Medicine, Mashhad University of Medical Sciences, Madhhad, I.R.Iran 1. Department of Neurology, Faculty of Medicine, Mashhad University of Medical Sciences, Madhhad, I.R.Iran 1. Department of Neurology, Faculty of Medicine, Mashhad University of Medical Sciences, Madhhad, I.R.Iran 1 12 2019 21 1 378 382 21 04 2019 25 09 2019 BACKGROUND AND OBJECTIVE: Mycobacterium tuberculosis affects millions of people in the world and Iran is one of the endemic areas. This disease has a wide range of manifestations, which is pulmonary in 80 – 85% of cases and extrapulmonary in 15 – 20% of cases. The most notable extrapulmonary infection sites are lymph nodes, pleura, genitourinary system and digestive system. Considering the prevalence of tuberculosis in Iran, familiarity with the uncommon manifestations of this infection seems to be essential for timely diagnosis and treatment. Therefore, a rare extrapulmonary manifestation of tuberculosis is reported here. CASE REPORT: The patient was a 64 – year – old woman who was hospitalized due to fever, icterus, decreased consciousness and progressive asymmetrical weakness of the limbs. Symptoms of focal neurologic deficits and heart murmurs were found in examinations. During the tests, including brain and spinal imaging, cerebrospinal fluid analysis, computed tomography of the abdomen and pelvis, and echocardiography, extrapulmonary tuberculosis (cerebral abscess, spondylitis, liver and spleen abscess, psoas muscle abscess, and possible endocarditis) was confirmed. Four-drug regimen including isoniazid, rifampin, ethambutol, and pyrazinamide with vitamin B6 was started for two months, and two-drug regimen continued for 10 months. At the end of the course of treatment, the patient was able to walk with the cane in good general condition.  CONCLUSION: Considering the reported case, paying attention to some of the more uncommon manifestations of the disease can prevent mortality and disability. 8382 Obstetrics and Gynecology Assessment of Effective Factors In Recurrent Implantation Failure (RIF) Following Assisted Reproductive Technology (ART) Basirat Z Kashifard M Aghaei Z Mahouti T Jorsaraei S Gh Golsorakhtabar amiri M 1. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 2. Cancer Research Center, Health Research Institute, Babol University of Medical Sciences, Babol I.R.Iran 3. Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran 1. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 1. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 1. Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 1 12 2019 21 1 383 389 17 12 2018 25 09 2019 BACKGROUND AND OBJECTIVE: Despite many advances have been made in the assisted reproduction techniques (ART), implantation rate after embryo transfer has not increased significantly. Therefore, this study aims to determine the factors involved in recurrent implantation failure (RIF) following ART. METHODS: In this retrospective study, 80 infertile patients with a history of at least 3 recurrent implantation failure (RIF) following assisted reproductive technology (ART) were referred to Fatemezahra Infertility Center in Babol from March 2006 to March 2013 were selected. The control group also included 80 women who became pregnant following the first IVF. Associated factors of infertility, endocrine disorder and endometriosis were recorded and their effect on RIF was assessed in both groups. FINDINGS: The mean BMI was 26.39±3.63 in the case group and 26.77±4.49 in the control group. Body mass index (BMI) >30 increased the risk of RIF significantly (p=0.001). After adjusting for the effects of other possible confounding factors, the odds ratio of obesity on RIF was 1.09(0.91-1.19) 95% CI which was not significant (p=0.06). There were no significant differences among type and cause of infertility, endocrine abnormalities, uterine malformations, endometriosis and polycystic ovary with RIF. CONCLUSION: According to our findings, BMI, type and cause of infertility, endocrine disorders, uterine abnormalities, endometriosis, and polycystic ovary were not effective on recurrent implantation failure.   8339 Epidemiology Trends in the Causes of Maternal Mortality in Iran and other Countries in the Region During the Years 1990 – 2016 Tajvar M Yaseri M Mosadeghrad AM Zalvand R 1. Department of Health Management and Economics, School of Public Health, Tehran University of Medical Sciences, Tehran, I.R.Iran 2. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, I.R.Iran 1. Department of Health Management and Economics, School of Public Health, Tehran University of Medical Sciences, Tehran, I.R.Iran 1. Department of Health Management and Economics, School of Public Health, Tehran University of Medical Sciences, Tehran, I.R.Iran 1 12 2019 21 1 390 397 28 11 2018 27 08 2019 BACKGROUND AND OBJECTIVE: Understanding the causes and frequency of maternal mortality and their occurrence over time is the first important step in policymaking to reduce maternal mortality. The purpose of this study was to compare the trends in the causes of maternal mortality in Iran and other countries in the region including 30 countries. METHODS: In this longitudinal, retrospective cohort study, the trends in causes of maternal mortality between 1990 and 2016 were evaluated. The study data were collected from the Global Burden of Disease Study (GBD) website and a secondary analysis was performed using Multilevel Linear Regression Models. In this study, "time" is the independent variable and "percentage of causes of death" is the dependent variable. FINDINGS: Among the direct causes of death, hemorrhage, despite a significant decreasing trend, was the most important cause of maternal mortality in Iran with a coefficient of 0.28 (CI = -0.25, -0.31, p<0.001). Other direct causes of death, including hypertension, infection, abortion and ectopic pregnancy, labor obstruction, and uterine rupture and death due to delays, all showed a significant decreasing trend during the annual period with regression coefficients of 0.17, 0.16, 0.15, 0.13, and 0.02, respectively (p<0.001). After hemorrhage, indirect causes and deaths due to other reasons were respectively ranked as second and third causes. The trends in almost all causes of death in Iran have declined significantly over this period compared to other countries in the region. CONCLUSION: The results of the study showed that the trends in all causes of maternal mortality has decreased significantly in Iran over the past 26 years, but this decline was lower compared to other countries in the region. 8641 Anesthesia Comparing the Effects of Propofol Infusion and Inhalation Isoflurane on Hemodynamic Variations and Depth of Anesthesia in Cataract Surgery Patients khakzad M Rabiee M Alijanpour E Banihashem N ghasemi jirdehi M Khafri S khakzad M 1. Babol University of Medical Sciences, Babol, I.R.Iran 2. Clinical Research Development Unite of Rouhani Hospital, Babol University of Medical Sciences, Babol, I.R.Iran 2. Clinical Research Development Unite of Rouhani Hospital, Babol University of Medical Sciences, Babol,I.R.Iran 2. Clinical Research Development Unite of Rouhani Hospital, Babol University of Medical Sciences, Babol,I.R.Iran 3. Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 4. Cancer Research Center, Health Research Institute, Babol University of Medical Sciences, Babol I.R.Iran 5. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran 1 12 2019 21 1 398 402 09 04 2019 21 09 2019 BACKGROUND AND OBJECTIVE: In cataract surgery, given that most patients are older people, surgery with general anesthesia requires better hemodynamic control along with maintaining the depth of anesthesia. The present study was conducted to compare the effects of propofol and isoflurane on hemodynamic variations and depth of anesthesia in cataract surgery patients. METHODS: The present clinical trial was conducted among 60 patients who were cataract surgery candidates. The patients were randomly assigned to propofol group (n = 30) and isoflurane group (n = 30). One µg/kg fentanyl and one mg/kg intravenous lidocaine were administered in both groups and anesthesia was induced using 1.5 – 2.5 mg/kg propofol. In order to maintain anesthesia, 50 – 75 µg/kg/min propofol was administered in the first group and 1% isoflurane was administered in the second group. Depth of anesthesia, hemodynamic variations, recovery time, wake-up time, nausea and vomiting were recorded and compared in the two groups. FINDINGS: Depth of anesthesia was similarly below 60 in both groups at different times. Hemodynamic variations were not significantly different in the two groups. Mean recovery time in propofol and isoflurane groups was 20.56 and 15.4 minutes, respectively (p<0.001), and wake-up time in the two groups was 8.83 and 7.16 minutes, respectively (p= 0.004). CONCLUSION: The results showed that there was no difference between the effects of these two drugs on hemodynamics and depth of anesthesia, but recovery time and wake-up time in propofol group were significantly higher than isoflurane group.