Journal of Babol University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی بابل
J Babol Univ Med Sci
Medical Sciences
http://jbums.org
1
admin
1561-4107
2251-7170
10.22088/jbums
fa
jalali
1394
7
1
gregorian
2015
10
1
17
10
online
1
fulltext
fa
بررسی تاثیر هیپوکسی بر تغییرات بیان 107miR- در رده های سلولی سرطان معده 45MKN و AGS
An Investigation of the Effect of Hypoxia on Expression of 107-miR in Gastric Cancer Cell Lines MKN-45 and AGS
ژنتیک، بیولوژی سلولی و مولکولی
Genetics, Cell and Molecular Biology
تحقیقی
Research
<p dir="rtl" style="margin: 0cm 0cm 0.0001pt text-align: right line-height: 16pt direction: rtl unicode-bidi: embed"><strong><span lang="AR-SA" style="font-size: 10pt color: blue">سابقه و هدف:</span></strong><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">هیپوکسی در تومورهای جامد، علت اصلی مقاومت به درمان در سرطان است.
بنابراین شناخت اندیکاتورهای مناسب جهت آگاهی از وضعیت هیپوکسی در تومور برای
انجام روش های صحیح درمانی و آگاهی از پیش اگهی تومور بسیار مفید و ضروری است. هدف
از انجام این تحقیق بررسی تغییر بیان<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">miR-</span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span>107<span class="apple-converted-space"> </span>به عنوان بیومارکر و اندیکاتور هیپوکسی
و ارتباط آن با هیپوکسی در سلول های سرطان معده می باشد.</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)"><o:p /></span></p>
<p dir="rtl" style="margin: 0cm 0cm 0.0001pt line-height: 16pt direction: rtl unicode-bidi: embed text-align: start"><strong><span lang="AR-SA" style="font-size: 10pt color: blue">مواد و روشها:</span></strong><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">در این تحقیق، رده سلولی سرطان معده 45</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">MKN</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">و<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">AGS</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">از انستیتو پاستور تهران تهیه و درشرایط اکسیژن نرمال(95%<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">O<sub>2</sub></span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">و 5%<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">CO<sub>2</sub></span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span>) و هیپوکسی
(95%<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">N<sub>2</sub></span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">و 5%<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">CO<sub>2</sub></span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span>) کشت داده
شدند و در فواصل زمانی 24 و 48 ساعت بررسی بیان 107</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">miR-</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">و<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">HIF-1α</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">انجام گرفت.<o:p /></span></p>
<p dir="rtl" style="margin: 0cm 0cm 0.0001pt line-height: 16pt direction: rtl unicode-bidi: embed text-align: start"><strong><span lang="AR-SA" style="font-size: 10pt color: blue">یافتهها:</span></strong><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: blue"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">با القای هیپوکسی در رده های سلولی بیان<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">HIF-1α</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">در رده 45</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">MKN</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">با افزایش مدت زمان تیمار (24و48 ساعت)، به ترتیب 2/3 و 3/8 برابر
افزایش پیدا کرد (0/002 </span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">p=</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">و<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">p=</span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span>0/04) و در رده<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">AGS</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">2/2 و 3/8 برابر افزایش یافت (0/005 </span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">p=</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">و 0/03 </span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">p=</span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span>). همچنین بیان افزایش یافته<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">miR-</span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span>107<span class="apple-converted-space"> </span>در
رده 45</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">MKN</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">در این بازه های زمانی به ترتیب 2/2 و 3/1 برابر افزایش
مشاهده شد (0/0001 </span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">p=</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">و 0/01 </span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">p=</span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span>) و در رده<span class="apple-converted-space"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">AGS</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">به ترتیب 2/4 و 4 برابر افزایش داشت (0/0001 </span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">p=</span><span dir="rtl"></span><span class="apple-converted-space"><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">و 0/002 </span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">p=</span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span>).<o:p /></span></p>
<p dir="rtl" style="margin: 0cm 0cm 0.0001pt line-height: 16pt direction: rtl unicode-bidi: embed text-align: start"><strong><span lang="AR-SA" style="font-size: 10pt color: blue">نتیجه گیری:</span></strong><span class="apple-converted-space"><b><span lang="AR-SA" style="font-size: 10pt color: blue"> </span></b></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)">نتایج مطالعه نشان داد که القای هیپوکسی در رده های سلولی سرطان
معده بر میزان تغییرات بیان </span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51)">miR</span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51)"><span dir="rtl"></span>-107<span class="apple-converted-space"> </span>موثر
است.<o:p /></span></p>
<p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><b><span style="font-size: 10pt color: blue">BACKGROUND AND OBJECTIVE: </span></b><span style="font-size: 10pt">Hypoxia in solid
tumors is the major cause of cancer treatment resistance. Thus, identifying the
appropriate indicators of hypoxia in tumors is of great importance for
appropriate tumor prognosis and choosing the best treatment methods. This study
aims to investigate the modulation of miR-107 expression, as a biomarker of
hypoxia and its association with gastric cancer cells.<o:p /></span></p>
<p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><b><span style="font-size: 10pt color: blue">METHODS: </span></b><span style="font-size: 10pt">MKN45 and AGS gastric cancer cells were
purchased from Tehran Pasteur Institute and then, were cultured under normal
oxygen conditions (CO<sub>2</sub> 5% and O<sub>2</sub> 95%) and hypoxia (CO<sub>2</sub>
5% and N<sub>2</sub> 95%). Finally, miR-107 and HIF-1-α expressions were
evaluated every 24 to 48 hours</span><span style="font-size: 10pt">.<o:p /></span></p>
<p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><b><span style="font-size: 10pt color: blue">FINDINGS: </span></b><span style="font-size: 10pt">The results of the study showed that
through hypoxia induction, HIF-1-α expression in MKN-45 cell lines increased by
extending treatment duration (24 and 48 hours) 2.3 and 3.8 times, respectively,
(p=0.04 and p=0.002), and in AGS cell lines HIF-1-α expression increased 2.2
and 3.8 times (p=0.03 and p=0.005). In addition, miR-107 expression in MKN-45
increased during this time by 2.2 and 3.1 times (p=0.01 and p=0.0001), while in
AGS cell lines it was by 2.4 and 4 times (p=0.002 and p=0.0001).<o:p /></span></p>
<p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><b><span style="font-size: 10pt color: blue">CONCLUSION:</span></b><span style="font-size: 10pt"> </span><span style="font-size: 10pt">It was demonstrated that hypoxia induction in gastric cancer
cells could modulate the expression of miR-107</span><span style="font-size: 10pt">. <o:p /></span></p>
سرطان معده, هیپوکسی, HIF-1 آلفا,107miR-.
Gastric cancer, HIF-1-alpha, Hypoxia, miR-107
39
45
http://jbums.org/browse.php?a_code=A-10-1848-1&slc_lang=fa&sid=1
N
Ayremlou
نوشین
آیرملو
100319475328460011750
100319475328460011750
No
H
Mozdarani
حسین
مزدارانی
mozdarah@modares.ac.ir
100319475328460011751
100319475328460011751
Yes
SJ
Mowla
سید جواد
مولا
100319475328460011752
100319475328460011752
No
A
Delavari
علیرضا
دلاوری
100319475328460011753
100319475328460011753
No