Journal of Babol University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی بابل
J Babol Univ Med Sci
Medical Sciences
http://jbums.org
1
admin
1561-4107
2251-7170
10.22088/jbums
fa
jalali
1394
7
1
gregorian
2015
10
1
17
10
online
1
fulltext
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تشخیص سریع blaTEM در ایزوله های کلبسیلا به روش مولکولی PCR
Early Detection of blaTEM in Klebsiella Isolates by the Molecular Polymerase Chain Reaction Method
ژنتیک، بیولوژی سلولی و مولکولی
Genetics, Cell and Molecular Biology
كاربردي
Applicable
<blockquote style="margin: 0px 0px 0px 40px border: none padding: 0px">
<p dir="rtl" style="margin: 0cm 0cm 0.0001pt text-align: right line-height: 15pt direction: rtl unicode-bidi: embed background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><strong><span lang="AR-SA" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">سابقه و هدف</span></strong><strong><span dir="ltr" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></strong><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">اطلاع از وجود ژن های مولد</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">بتالاکتامازهای وسیع الطیف در باکتری هایی چون کلبسیلا از نظر بیماری زایی و نیز میزان شیوع آنها در جوامع، در پیشگیری و درمان عفونت های ناشی از آنها کمک می نماید.هدف از این مطالعه شناسایی سریع ژن مولد</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">bla<sub>TEM</sub><span class="apple-converted-space"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">در کلبسیلا با استفاده از تکنیک</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">PCR<span class="apple-converted-space"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">می باشد</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.</span><span dir="ltr" style="font-size: 7.5pt"><u1:p><!--stripped--><!--stripped--></u1:p></span></p>
<p dir="rtl" style="margin: 0cm 0cm 0.0001pt line-height: 15pt direction: rtl unicode-bidi: embed text-align: start background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><strong><span lang="AR-SA" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">مواد و روشها</span></strong><strong><span dir="ltr" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></strong><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">در این مطالعه مقطعی، از فروردین ماه لغایت مهرماه 1392 تعداد 70 ایزوله باکتری کلبسیلا از نمونه دهای بالینی شامل (زخم، ادرار، خلط و خون) بر اساس تست هاس بیوشیمیایی( وضعیت غیر تخمیری و تولید گاز در محیط</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">TSI</span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">، منفی بودن اندول، حرکت و</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">MR</span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">، تست اوره آز و</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">VP<span class="apple-converted-space"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">مثبت) مجزا گردیدند. سپس فراوانی سویه های تولید کننده بتالاکتامازهای وسیع الطیف، با</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">Combined disk</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 7.5pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">تعیین گردید</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.<span class="apple-converted-space"> </span>DNA<span class="apple-converted-space"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">با روش جوشانیدن استخراج شده و از نظروجود ژن</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">TEM<span class="apple-converted-space"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">توسط روش</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">PCR<span class="apple-converted-space"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">مورد بررسی قرار گرفت</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.<u1:p></u1:p></span><span lang="AR-SA" style="font-size: 7.5pt"><!--stripped--><!--stripped--></span></p>
<p dir="rtl" style="margin: 0cm 0cm 0.0001pt line-height: 15pt direction: rtl unicode-bidi: embed text-align: start background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><strong><span lang="AR-SA" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">یافته</span></strong><strong><span dir="ltr" lang="AR-SA" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></strong><strong><span lang="AR-SA" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">ها</span></strong><strong><span dir="ltr" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></strong><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">از مجموع 70 ایزوله کلبسیلا، در 11 نمونه فنوتیپ بتالاکتاماز وسیع الطیف مشاهده شد که از این تعداد ، 10 نمونه دارای ژن مقاومت بتالاکتاماز</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">TEM<span class="apple-converted-space"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">بودند. همچنین از 59 نمونه</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">ESBL<span class="apple-converted-space"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">منفی در آنتی بیوگرام، 9 نمونه (26%) به وسیله تست</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">PCR<span class="apple-converted-space"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">از جهت ژن</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">bla<sub>TEM</sub><span class="apple-converted-space"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">مثبت تشخیص داده شد</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.<u1:p></u1:p></span><span lang="AR-SA" style="font-size: 7.5pt"><!--stripped--><!--stripped--></span></p>
<p dir="rtl" style="margin: 0cm 0cm 0.0001pt text-align: right line-height: 13.05pt direction: rtl unicode-bidi: embed"></p>
<p dir="rtl" style="margin: 0cm 0cm 0.0001pt line-height: 15pt direction: rtl unicode-bidi: embed text-align: start background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><strong><span lang="AR-SA" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">نتیجه گیری</span></strong><strong><span dir="ltr" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></strong><span class="apple-converted-space"><b><span dir="ltr" style="font-size: 10pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></b></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">با توجه به شیوع روزافزون سویه های تولید کننده بتالاکتامازهای وسیع الطیف و تشخیص ضعیف مقاومت آنتی بیوتیکی توسط روش سنتی آنتی بیوگرام، استفاده از تکنیک های دقیق تر از جمله</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">PCR</span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">،</span><span class="apple-converted-space"><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span lang="AR-SA" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">قویا توصیه می شود</span><span dir="ltr" style="font-size: 10pt color: rgb(51, 51, 51) background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.</span><span lang="AR-SA" style="font-size: 7.5pt"><!--stripped--><!--stripped--></span></p>
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<p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><b><span style="font-size: 10pt color: blue">BACKGROUND AND OBJECTIVE: </span></b><span style="font-size: 10pt">Obtaining information regarding pathogenesis and prevalence of extended spectrum beta-lactamase (ESBL) producing genes seems to be necessary, since it can promote prevention modalities and treatment of the infections caused by bacterias such as Klebsiella. The aim of this study was early identification of the blaTEM gene in Klebsiella, using polymerase chain reaction (PCR) technique</span><span style="font-size: 10pt">.<!--stripped--><!--stripped--></span></p>
<p class="MsoNoSpacing" style="line-height: 16pt"><b><span lang="X-NONE" style="font-size: 10pt color: blue">METHODS: </span></b><span lang="X-NONE" style="font-size: 10pt">In this cross-sectional study, conducted form April to September 2013, 70 Klebsiella isolates were extracted from clinical samples (i.e., wound, urine, sputum and blood) using biochemical tests, including non-state fermentation and triple sugar iron, negative indole, motile and methyl red, as well as positive Voges–Proskauer and urease tests. Subsequently, the frequency of ESBL producing strains was determined by means of combined disk method. DNA was extracted by boiling and was investigated for the presence of TEM gene using the PCR approach</span><span lang="X-NONE" style="font-size: 10pt">.<!--stripped--><!--stripped--></span></p>
<p class="MsoNoSpacing" style="line-height: 16pt"><b><span lang="X-NONE" style="font-size: 10pt color: blue">FINDINGS: </span></b><span lang="X-NONE" style="font-size: 10pt">In the 70 Klebsiella isolates, 11 cases of ESBL phenotype were observed, of which 10 cases contained TEM </span><span lang="X-NONE" style="font-size: 10pt">beta</span><span lang="X-NONE" style="font-size: 10pt">-lactamase resistance gene. In addition, 9 out of 59 samples (26%) of negative ESBL in antibiogram, were determined positive in terms of blaTEM gene using PCR method</span><span lang="X-NONE" style="font-size: 10pt">.<!--stripped--><!--stripped--></span></p>
<p><b><span style="font-size: 10pt font-family: 'Times New Roman', serif color: blue">CONCLUSION:</span></b><span style="font-size: 10pt font-family: 'Times New Roman', serif"> </span><span style="font-size: 10pt font-family: 'Times New Roman', serif">Given the increasing prevalence of ESBL producing strains and poor diagnosis rate of antibiotic resistance through antibiogram method, applying more accurate techniques such as PCR is highly recommended.</span></p>
ژن مقاومت blaTEM, بتالاکتامازهای وسیع الطیف, واکنش زنجیره ای پلیمراز, آنتی بیوگرام
BlaTEM Resistance Gene, Extended Spectrum Beta-lactamases, Polymerase Chain Reaction, Antibiogram
46
52
http://jbums.org/browse.php?a_code=A-10-1829-1&slc_lang=fa&sid=1
S
Bostandoost nikoo
سارا
بستان دوست نیکو
100319475328460012267
100319475328460012267
No
MH
Shahhosseiny
محمد حسن
شاه حسینی
shahhosseiny@yahoo.com
100319475328460012268
100319475328460012268
Yes
MR
Zolfaghari
محمد رضا
ذوالفقاری
100319475328460012269
100319475328460012269
No
M
Rahbar
محمد
رهبر
100319475328460012270
100319475328460012270
No