Journal of Babol University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی بابل
J Babol Univ Med Sci
Medical Sciences
http://jbums.org
1
admin
1561-4107
2251-7170
10.22088/jbums
fa
jalali
1394
1
1
gregorian
2015
4
1
17
4
online
1
fulltext
fa
اختلال یادگیری و حافظه ناشی از تزریق داخل هیپوکمپی اسیداسکوربیک و اسکوربات اکسیداز در مدل ماز آبی موریس
Learning and Memory Impairment Induced by the Injection of Ascorbic Acid and Ascorbate Oxidase into the Hippocampus in the Morris Water Maze
فیزیولوژی
Physiology
تجربی
Experimental
<p class="MsoNormal" dir="rtl" style="line-height: 16pt"><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue">سابقه</span></b><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue"> </span></b><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue">و</span></b><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue"> </span></b><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue">هدف</span></b><b><span style="font-size: 10pt font-family: '2 Mitra' color: blue">:</span></b><span style="font-size: 10pt font-family: '2 Mitra'"> </span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">اسید اسکوربیک دارای طیف وسیعی از عملکرد ها در سیستم عصبی مرکزی از جمله نورومودیولاتور و آنتی اکسیدانی می باشد. آسکوربیک اسید با نوروترانسمیترهای دخیل در یادگیری و حافظه مداخله دارد. در این مطالعه اثر تزریق آن وآنزیم حذف کننده اش در ناحیه </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">CA1</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> هیپوکمپ بر یادگیری و حافظه فضایی مورد بررسی قرار گرفت.<!--stripped--><!--stripped--></span></p>
<p class="MsoNormal" dir="rtl" style="line-height: 16pt"><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue">مواد</span></b><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue"> </span></b><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue">و</span></b><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue"> </span></b><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue">روشها</span></b><b><span style="font-size: 10pt font-family: '2 Mitra' color: blue">:</span></b><span style="font-size: 10pt font-family: '2 Mitra'"> </span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">در این مطالعه تجربی از </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">49</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> سر موش صحرایی نژاد ویستار (</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">mg/kg</span><span style="font-size: 10pt font-family: '2 Mitra'"> </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">270</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">-</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">220</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">)در قالب هفت گروه شامل: کنترل، شاهد (دریافت کننده حلال داروها)، اسکوربات اکسیداز (</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">g/kg</span><span lang="FA" style="font-size: 10pt font-family: 'Times New Roman', serif">µ</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">0.4</span><span style="font-size: 10pt font-family: '2 Mitra'"> </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">0.2,</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">)، اسید اسکوربیک(</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">g/kg</span><span lang="FA" style="font-size: 10pt font-family: 'Times New Roman', serif">µ</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">24</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">،</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">12</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">) و اسکوربات اکسیداز غیرفعال(</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">g/kg</span><span lang="FA" style="font-size: 10pt font-family: 'Times New Roman', serif">µ</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">0.2</span><span style="font-size: 10pt font-family: '2 Mitra'">)</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> استفاده شد. کانول گذاری دو طرفه در ناحیه</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">CA1</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> هیپوکمپ با استفاده از دستگاه استریوتکسی انجام شد. بعد از یک هفته دوره ریکاوری تزریق داروها با حجم یک میکرولیتر با سرنگ همیلتون صورت گرفت. حافظه و یادگیری فضایی با استفاده از ماز آبی موریس اندازه گیری شد</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">.</span><span style="font-size: 10pt font-family: '2 Mitra'"><!--stripped--><!--stripped--></span></p>
<p class="MsoNormal" dir="rtl" style="line-height: 16pt"><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue">یافته</span></b><b><span style="font-size: 10pt font-family: '2 Mitra' color: blue">­</span></b><b><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra' color: blue">ها</span></b><b><span style="font-size: 10pt font-family: '2 Mitra' color: blue">:</span></b><b><span style="font-size: 10pt font-family: '2 Mitra' color: blue"> </span></b><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">نتایج نشان داد اسید اسکوربیک در دوز </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">g/kg</span><span lang="FA" style="font-size: 10pt font-family: 'Times New Roman', serif">µ</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">12</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> مسافت طی شده</span><span dir="ltr" lang="FA" style="font-size: 10pt font-family: 'Times New Roman', serif"> </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">(1012.98±63.55) </span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> و زمان</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif"> (55.48±2.38)</span><span style="font-size: 10pt font-family: '2 Mitra'"> <span lang="FA">رسیدن به سکو پنهان را در مقایسه با کنترل</span></span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">(45.9±1.84) (633.33±18.46)</span><span style="font-size: 10pt font-family: '2 Mitra'"> <span lang="FA">افزایش می دهد </span></span><span style="font-size: 10pt font-family: '2 Mitra'">(</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">p<0/05</span><span style="font-size: 10pt font-family: '2 Mitra'">)</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">. همچنین مسافت طی شده (</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">1123.73±108.89</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">) و تاخیر رسیدن به سکو پنهان </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">(57.31±1.18)</span><span style="font-size: 10pt font-family: '2 Mitra'"> <span lang="FA">با دوز </span></span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">g/kg</span><span lang="FA" style="font-size: 10pt font-family: 'Times New Roman', serif">µ</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> 24 اسید اسکوربیک در مقایسه با گروه کنترل افزایش یافت </span><span style="font-size: 10pt font-family: '2 Mitra'">(</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">p<0.01</span><span style="font-size: 10pt font-family: '2 Mitra'">).</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> مشخص شد اسکوربات اکسیداز در هر دو دوز </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">g/kg</span><span lang="FA" style="font-size: 10pt font-family: 'Times New Roman', serif">µ</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">0.2</span><span style="font-size: 10pt font-family: '2 Mitra'"> </span><span style="font-size: 10pt font-family: '2 Mitra'">(</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">p<0.01</span><span style="font-size: 10pt font-family: '2 Mitra'">)</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> و </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">g/kg</span><span lang="FA" style="font-size: 10pt font-family: 'Times New Roman', serif">µ</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">0.4</span><span style="font-size: 10pt font-family: '2 Mitra'"> </span><span style="font-size: 10pt font-family: '2 Mitra'">(</span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">p<0.001</span><span style="font-size: 10pt font-family: '2 Mitra'">)</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> مسافت و زمان رسیدن به سکو را نسبت به گروه کنترل افزایش می دهد.<!--stripped--><!--stripped--></span></p>
<p class="MsoNormal" dir="rtl" style="line-height: 16pt direction: rtl"></p>
<p class="MsoNormal" dir="rtl"><b><span lang="FA" style="font-size: 10pt font-family: '2 Mitra' color: blue">نتیجه</span></b><b><span dir="ltr" lang="FA" style="font-size: 10pt font-family: 'Times New Roman', serif color: blue"> </span></b><b><span lang="FA" style="font-size: 10pt font-family: '2 Mitra' color: blue">گیری:</span></b><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> نتایج مطالعه نشان داد که </span><span lang="AR-LY" style="font-size: 10pt font-family: '2 Mitra'">تزریق اسید اسکوربیک و آنزیم حذف کننده آن در ناحیه </span><span dir="ltr" style="font-size: 10pt font-family: 'Times New Roman', serif">CA1</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'"> هیپوکمپ هر دو منجر به کاهش یادگیری و حافظه فضایی می گردد</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra'">.</span></p>
<p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><b><span style="font-size: 10pt font-family: 'Times New Roman', serif color: blue">BACKGROUND AND OBJECTIVE:</span></b><span style="font-size: 10pt font-family: 'Times New Roman', serif"> Ascorbic acid has a wide range of functions in the central nervous system such as neuromodulator and antioxidant. Ascorbic acid intervenes with the neurotransmitters involved in learning and memory. In this study, we examined the effects of its injection and its removal enzyme in the hippocampal CA1 region on spatial learning and retention.<!--stripped--><!--stripped--></span></p>
<p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><b><span style="font-size: 10pt font-family: 'Times New Roman', serif color: blue">METHODS:</span></b><span style="font-size: 10pt font-family: 'Times New Roman', serif"> We used 49 Wistar rats in this study (220-270 mg/kg) and we divided them into seven groups including: control, sham (recipients of solvent), ascorbate oxidase (0.2, 0.4 µg/kg), ascorbic acid (24, 12µg/kg), and inactive ascorbate oxidase (0.2 µg/kg). Bilateral cannula was performed in the hippocampal CA1 region using stereotaxy device. After one week of recovery, one microliter of the drugs was injected by a Hamilton syringe. Spatial learning and retention was measured by using the Morris water maze.</span><span dir="rtl" style="font-size: 10pt font-family: '2 Mitra'"> </span><span dir="rtl" style="font-size: 10pt font-family: 'Times New Roman', serif background: white"><!--stripped--><!--stripped--></span></p>
<p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><b><span style="font-size: 10pt font-family: 'Times New Roman', serif color: blue">FINDINGS:</span></b><span style="font-size: 10pt font-family: 'Times New Roman', serif"> </span><span style="font-size: 10pt font-family: 'Times New Roman', serif">The results show that 12 µg/kg dose of ascorbic acid increases mileage (1012.98±63.55) and escape latency (55.48±2.38) compared to the control group (633.33±18.46) (45.9±1.84) (p<0.05). Also mileage (1123.73±108.89) and escape latency delay (57.31±1.18) were raised with 24 µg/kg dose of ascorbic acid compared to the control group (p<0.01). It was determined that ascorbate oxidase with both 0.2µg/kg (p<0.01) and 0.4µg/kg (p<0.001) dose increased the mileage and escape latency compared to the control group</span><span style="font-size: 10pt font-family: 'Times New Roman', serif">.<span dir="rtl" lang="FA"><!--stripped--><!--stripped--></span></span></p>
<p><b><span style="font-size: 10pt font-family: 'Times New Roman', serif color: blue">CONCLUSION:</span></b><span style="font-size: 10pt font-family: 'Times New Roman', serif"> The results showed that the injection of ascorbic acid and its removal enzyme in the hippocampal CA1 region leads to spatial learning and retention loss.</span></p>
یادگیری و حافظه فضایی, ماز آبی موریس, آسکوربات اکسیداز, اسید اسکوربیک, هیپوکمپ
Spatial Learning and Retention, The Morris Water Maze, Ascorbate Oxide, Ascorbic Acid, Hippocampus.
36
43
http://jbums.org/browse.php?a_code=A-10-1472-2&slc_lang=fa&sid=1
sh
Jamali
شعله
جمالی
100319475328460013890
100319475328460013890
No
M
Abbasnejad
مهدی
عباس نژاد
mabbas@mail.uk.ac.ir
100319475328460013891
100319475328460013891
Yes
S
Esmaeili mahani
سعید
اسماعیلی ماهانی
100319475328460013892
100319475328460013892
No
A
Badoei-Dalfard
ارسطو
بدویی دلفارد
100319475328460013893
100319475328460013893
No
R
Kooshki
راضیه
کوشکی
100319475328460013894
100319475328460013894
No