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Combination Effect of Methadone and Haloperidol on Morphine Withdrawal-Induced Anxiety in Male Mice
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E Yaghoutpoor *   , Z Kiasalari , M Khalili , S Vahidi |
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Abstract: (11561 Views) |
BACKGROUND AND OBJECTIVE: Today addiction to morphine is one of the important problems in human societies. Incidence of anxiety as a withdrawal syndrome causes quit of chronic morphine use being difficult for addicted persons. For this reason, in this study the combined effect of methadone and haloperidol was examined on the anxiety induced by withdrawal of morphine in mice.
METHODS: In this experimental study, 98 NMRI male mice were randomly divided into chronic and acute groups. Each group was divided into seven sub-groups: saline, morphine (control), methadone (10mg/kg), haloperidol (0.3mg/kg), methadone+haloperidol (5mg/kg and 0.15mg/kg), methadone+haloperidol ratio of 2 to 1 (7mg/kg and 0.1mg/kg) and methadone+haloperidol ratio of 1 to 2 (3.5mg/kg and 0.2mg/kg). Except the saline group the other mice were addicted with gradually increasing doses of morphine for 8 consecutive days. All drugs in the chronic group were injected 30 minutes before morphine injection for 8 days and in the acute group a single dose 30 minutes before morphine injection in the 8th day (test day). For the assessment of anxiety in mice, elevated plus-maze was used after injecting naloxone in the test day.
FINDINGS: The result showed that in acute injection of drugs, percentage of open arm entries and percentage of time spent in open arms in the groups: methadone (37.72±3.34 and 36.79±4.03, p<0.05) haloperidol (41.33±3.70 and 41.23±6.26, p<0.01) methadone+haloperidol (39.66±4.80 and 43.14±5.85, p<0.05 and p<0.01), methadone2+ haloperidol1 (38.94±2.40 and 30.52±6.26, p<0.05), methadone1+haloperidol2 (40.80±2.50 and 37.53±4.48, p<0.01 and p<0.05) significantly increased in compared to the morphine group. But in chronic injection, only treatment groups including haloperidol (54.16±3.37 and 64.10±9.71, p<0.01 and p<0.001), methadone+haloperidol (53.61±2.54 and 51.68±4.65, p<0.01 and p<0.001), methadone1+haloperidol2 (52.61±2.97 and 54.63±4.65, p<0.05 and p<0.001) significantly reduced the anxiety caused by withdrawal of morphine.
CONCLUSION: The results show that probably a combination of methadone and haloperidol can reduce morphine withdrawal-induced anxiety in mice.
Please cite this article as follows:
Yaghoutpoor E, Kiasalari Z, Khalili M, Vahidi S. Combination effect of methadone and haloperidol on morphine withdrawal-induced anxiety in male mice. J Babol Univ Med Sci 2014 16(3): 41-48.
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| Keywords: Anxiety, Morphine, Methadone, Haloperidol. |
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Full-Text [PDF 452 kb]
(2015 Downloads)
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Type of Study: Research |
Subject:
Biochemical
Accepted: 2014/06/11 | Published: 2014/06/11
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