:: Volume 10, Issue 3 (8-2008) ::
JBUMS 2008, 10(3): 25-29 Back to browse issues page
RADIOSENSITIVITY OF HELA CELLS INFECTED WITH POLIO VIRUS IRRADIATED BY CO 60
F Seif *, MR Baiatiani,, MJ Tahmasebi Birgani,, A Sohrabi, F Hosseini
Abstract:   (44120 Views)
BACKGROUND AND OBJECTIVE: The main purpose of radiotherapy is exposing enough dose to tumoral volume and protecting the normal tissues around it. Tumor dose for each fraction in radiotherapy prescribes with tumoral tissue consideration. Since viral diseases can affect cell radiosensitivity, this study was done to evaluate the radiosensitivity of tumoral cell that infected with virus.
METHODS: In this study the radiosensitivity of HeLa cell with and without the viral agent of polio disease was obtained and compared after gamma radiation of cobalt 60. Different polio vaccine was used to infect cell with polio virus. Finally calculated the percentage of plating efficiency and relative survival at radiated and control cells with colonies countering.
FINDINGS: Results implicate that cell death increases up to 20-30% for contaminating with low concentrations of polio virus, 30-40% for moderate concentrations and 70-90% for high concentrations after 2Gy irradiation by cobalt 60.
CONCLUSION: Results of this study showed that existing of viral disease in tumoral region can increase radiosensitivity of tumoral cells. Therefore non cancer disease in region under irradiation should be noticed to prescribing dose fraction in radiotherapy of cancers.
Keywords: Radiosensitivity, HeLa cells, Polio virus
Full-Text [PDF 306 kb]   (1784 Downloads)    
Type of Study: Research | Subject: Biochemical
Accepted: 2014/06/3 | Published: 2014/06/3


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Volume 10, Issue 3 (8-2008) Back to browse issues page