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Effects of Access Cavity Design and Three Single-File Rotary Systems on Reduction of Enterococcus Faecalis Count in the Root Canal System
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M Emamgholipour    , S Mirzaee Rad * , A Pournajaf , SA Seyedmajidi |
| 1.Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran. , sinamirzaeerad@yahoo.com |
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Abstract: (49 Views) |
Background and Objective: This study aimed to assess the effects of access cavity design and three single-file rotary systems on reduction of Enterococcus faecalis (E. faecalis) count in the root canal system.
Methods: This in vitro study evaluated 60 extracted sound single-rooted single-canal mandibular premolars with long oval root canal cross-section. The teeth were randomly assigned to two groups (n=30) for preparation of traditional endodontic cavity (TEC) and conservative endodontic cavity (CEC). The root canals were prepared, and inoculated with E. faecalis (ATCC29212) for 4 weeks. Each group was randomly divided into three subgroups (n=10) for instrumentation with Reciproc, OneShape, and Only One File rotary systems. Bacterial samples were taken before (S1) and after (S2) instrumentation, using sterile paper points. Reduction in microbial load was calculated and reported in colony forming units (CFUs/mL). Data were analyzed by independent samples t-test and one-way ANOVA (alpha=0.05).
Findings: The reduction in microbial load was significantly greater in TEC design (99.85±0.04) than CEC design (99.76±0.06) (p<0.05). The reduction in microbial load was not significantly different among the three files in TEC or CEC design.
Conclusion: TEC design resulted in significantly greater reduction in E. faecalis count in the root canal system, but the three single-file rotary systems had no significant difference in this respect in any cavity design. |
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| Keywords: Enterococcus Faecalis, Root Canal Therapy, Bacterial Load. |
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Type of Study: Applicable |
Subject:
Dentistry (Endo)
Received: 2024/06/17 | Accepted: 2024/09/17
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