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Investigation of the Capillary Structure of Hippocampal Tissue of Healthy Rats after the Use of Urtica Dioica Hydroalcoholic Extract and Endurance Exercise
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F Gudarzi    , M Rahmati * , M Roozbahani , F Chehlcheraghi , A Farzaneh Hesari |
| 2.Department of Sports Sciences, School of Literature and Humanities, Lorestan University, Khorramabad, I.R.Iran. , rahmati.mas@lu.ac.ir |
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Abstract: (257 Views) |
Background and Objective: The structure and function of the hippocampal capillary are closely related to the regulation of neurogenesis and synaptic plasticity. Maintaining the health and proper organization of the hippocampal capillary network is essential for optimal hippocampal tissue function and cognitive performance. Given the beneficial effects of exercise on the body as well as the antioxidant properties of Urtica Dioica, this study was conducted to investigate the effect of endurance exercise and hydroalcoholic extract of Urtica Dioica on histomorphometric estimates of the capillaries of the hippocampal tissue of healthy rats.
Methods: In the present experimental study, 28 male Wistar rats were divided into 4 groups (n=7): control, endurance exercise (Ex), Urtica Dioica (UD), and endurance exercise+nettle (UD+Ex). After a week of familiarization with the laboratory conditions and treadmill running, endurance exercise was performed 5 days a week and daily gavage of UD hydroalcoholic extract (50 mg/kg) was administered for six weeks. 48 hours after the completion of the research protocol, the rats were sacrificed. Then the hippocampal tissue was removed and placed in 10% formalin for hematoxylin-eosin staining. After tissue staining, histomorphometric estimates, the number of sections, length, volume, and area of capillaries in the rat hippocampal tissue were examined and compared.
Findings: The results showed that the number of vascular sections and capillary volume were not significantly affected by endurance exercise and Urtica Dioica, but the capillary area in the UD+Ex group (1.24±0.21 mm2) significantly increased compared to the Ex (0.535±0.076 mm2) (p=0.013) and UD (0.5±0.08 mm2) (p=0.010) groups, although this increase was not different from the control group (0.815±0.15 mm2). A decrease in the capillary volume of the hippocampal tissue of the UD group was observed compared to the other groups (p=0.001).
Conclusion: The results of the study showed that the combination of UD and exercise helped maintain the capillary structure and function of hippocampal tissue in healthy rats. |
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| Keywords: Hippocampus, Endurance Exercise, Urtica Dioica, Vascular Structure. |
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Full-Text [PDF 478 kb]
(58 Downloads)
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Type of Study: Experimental |
Subject:
Physical Education and Sports Science
Received: 2024/02/12 | Accepted: 2024/06/11 | Published: 2025/05/4
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