:: Volume 24, Issue 1 (3-2022) ::
JBUMS 2022, 24(1): 95-102 Back to browse issues page
Association between HOTAIR rs1899663 G>T Gene Polymorphism and Thyroid Cancer Susceptibility
R Madadi Rad , N Pouladi * , A Nemati Bosharani , M Alizadeh
1.Department of Cellular and Molecular Biology, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, I.R.Iran. , srna52@gmail.com
Abstract:   (896 Views)
Background and Objective: Studies show that single nucleotide polymorphisms of the HOTAIR gene are associated with a variety of cancers, including colorectal, breast, and thyroid cancers. The product of the HOTAIR gene is a long non-coding RNA that is involved in regulating gene expression, cell cycle and apoptosis and is considered an oncogene. The present study was conducted to investigate the association between HOTAIR rs1899663 G>T gene polymorphism and thyroid cancer susceptibility in northwestern Iran.
Methods: In this case-control study, peripheral blood was obtained from 90 patients with thyroid cancer referred to Tabriz hospitals as well as 198 healthy individuals. After DNA extraction by saturated salt and proteinase K method, single nucleotide polymorphism of HOTAIR rs1899663 gene was examined by tetra-primers ARMS PCR. Then, allelic and genotypic frequencies of control and case groups were calculated and compared.
Findings: In this study, the genotypic frequencies of GG, GT and TT in the case group were 23.3%, 49% and 27.7%, and in the control group were 20.2%, 41% and 38.8%, respectively. Allele frequencies of G and T were 48% and 52.2% in the case group and 41% and 59% in the control group, respectively. Allelic and genotypic comparisons between case and control groups showed no significant relationship.
Conclusion: The results of the study showed that HOTAIR rs1899663 gene polymorphism is not associated with any of the clinicopathological features of thyroid cancer.
Keywords: Cancer, Thyroid, HOTAIR, Polymorphism.
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Type of Study: Case-Control | Subject: J
Received: 2021/05/17 | Accepted: 2021/09/18 | Published: 2022/04/25

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