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Neuroprotective Effect of Losartan after Traumatic Brain Injury in Male Rats Based on Marmarou Model
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Sh Shadi    , A Siahposht-Khachaki * , Gh Hooshmand |
| 1.Immunogenic Research Center, Mazandaran University of Medical Sciences, Sari, I.R.Iran. , siahposht@mazums.ac.ir |
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Abstract: (1379 Views) |
Background and Objective: Traumatic brain injury is the most important cause of death among young people in the world. Considering the neuroprotective effect of losartan on the recovery of brain damage, the present study was conducted to investigate the effects of losartan on neurological severity scores, blood-brain barrier health, cerebral edema, and histological changes after the induction of traumatic brain injury in male rats.
Methods: In this experimental study, 60 male Wistar rats weighing 250 to 280 grams were assigned into 7 groups: 1) Intact, 2) Surgery Sham, 3) Traumatic brain injury, 4) Losartan solvent (saline), 5) Low dose of losartan (5 mg), 6) Medium dose of losartan (10 mg), 7) High dose of losartan (20 mg) and 6-8 rats were used in each group. 30 minutes after brain trauma, different doses of losartan were injected intraperitoneally based on the Marmarou weight drop model. Neurobehavioral testing was performed, and cerebral edema and blood-brain barrier health were evaluated in all groups and the animal's brain was then used for tissue staining.
Findings: Losartan in doses of 5 mg (79.75±4.36) and 10 mg (70.375±5.60) was able to reduce cerebral edema (p<0.001) and neurological severity scores (11±1.41, and 12.75±1.90), and improve the animal's coordination on the balance beam task (11.125±3.313) and (6.875±2.94), respectively (p<0.001). In the dose of 10 mg, the effects were more significant (6.875±2.94) (p<0.001). In terms of histology, losartan decreased perivascular edema, and neuronal necrosis, and decreased astrocytic edema. Losartan had no effect in the dose of 20 mg (p>0.05).
Conclusion: The results of the study showed that losartan improves the outcomes of brain injury, which requires more studies in the future to investigate the mechanisms of this effect. |
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| Keywords: Losartan, Traumatic Brain Injury, Cerebral Edema, Rat. |
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Full-Text [PDF 661 kb]
(593 Downloads)
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Type of Study: Research |
Subject:
Physiology
Received: 2021/09/4 | Accepted: 2021/12/18 | Published: 2022/08/14
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