2024-03-29T10:45:08+03:30 http://jbums.org/browse.php?mag_id=435&slc_lang=fa&sid=1
435-5581 2024-03-29 10.1002
Journal of Babol University of Medical Sciences J Babol Univ Med Sci 1561-4107 2251-7170 10.22088/jbums 2015 17 10 The Relationship between Chronic Musculoskeletal Pain and Vitamin D ‎Deficiency in the Elderly Population of Amirkola, Iran M Alipour SR Hosseini hosseinirezaseyed@gmail.com P Saadat @gmail.com A Bijani BACKGROUND AND OBJECTIVE: Chronic musculoskeletal pain may  persist for more than three months and is often resistant to treatment. The aim of this study was to examine the relationship between chronic musculoskeletal pain and vitamin D deficiency in the elderly population of Amirkola, Iran.METHODS: This cross-sectional study was part of a health survey on the elderly population of Amirkola (AHAP=Amirkola Health and Ageing Project), including 1616 senior citizens, aged ≥ 60 years. Overall, 857 individuals without rheumatoid arthritis, cancer, depression, diabetes or osteoarthritis were enrolled in this study. Demographic data were collected and a questionnaire on chronic musculoskeletal pain was applied. The anatomical site of pain was determined by inquiring the elderly. The serum level of vitamin D was measured in two groups with and without chronic pain, using the ELISA method. Vitamin D deficiency, insufficiency and sufficiency were defined as serum vitamin D levels of < 20, 20-29.99 and ≥30 ng/ml, respectively.FINDINGS: Among 857 participants, 599 (69.9%) and 258 (30.1%) cases were male and female, respectively. Moreover, 666 participants (77.7%) complained of chronic pain. The most common sites of pain were the knees (54%), back (40%), feet (35.7%) and shoulders (27.5%), respectively. Also, 41.9% of the elderly had vitamin D deficiency. However, vitamin D level was not significantly different between the two groups (with and without chronic pain), and frequency of chronic pain did not significantly vary between these groups. Based on the logistic regression model, gender (OR=2.73, 95%CI=1.78-4.21, p=0.001), body mass index and physical activity (OR=0.42, 95% CI=0.28-0.62, p=0.001) had significant impacts on chronic pain. CONCLUSION: The results showed no significant relationship between vitamin D level and chronic musculoskeletal pain in the elderly population of Amirkola, Iran. Body mass index Chronic pain Elderly Physical activity Vitamin D 2015 10 01 7 14 http://jbums.org/article-1-5581-en.pdf 10.22088/jbums.17.10.7
435-5278 2024-03-29 10.1002
Journal of Babol University of Medical Sciences J Babol Univ Med Sci 1561-4107 2251-7170 10.22088/jbums 2015 17 10 Effects of Yakson Therapeutic Touch on the Behavioral Response of ‎Premature Infants F Eshghi eshghifateme@yahoo.com S Iranmanesh B Bahman Bijari F Borhani M Motamed Jahromi BACKGROUND AND OBJECTIVE: Due to incomplete physical development, premature infants are susceptible to various sources of stress. Therapeutic touch is considered as an effective method to reduce stress in neonates. This study aimed to compare the effects of Yakson therapeutic touch and routine nursing care on the behavioral response of premature infants. METHODS: This quasi-experimental study was conducted on 60 premature infants aged 26-34 weeks, admitted at the neonatal intensive care unit (NICU) of Afzalipour Hospital of Kerman, Iran. Subjects were randomly selected by minimization and equally divided into two groups of intervention and control (N=30). Infants in the intervention group received Yakson massage therapy for 5 days, twice daily for 15 minutes, and infants in the control group received routine care. Behavioral responses of infants, including the status of sleep, waking and restlessness, were evaluated using Anderson Behavioral State Scale (ABSS) before and after intervention. FINDINGS: In this study, mean scores of ABSS in preterm infants of the intervention and control groups was 4.83±0.14 and 10.63±0.14, respectively. Moreover, there was a significant difference between the scores of behavioral response after the intervention between the groups (p=0.001). CONCLUSION: According to the results of this study, increased scores of sleep status in the infants of the Yakson therapy group indicated that this method could be effective in the provision of nursing care for premature infants admitted at the NICU. Prematurity Yakson Human Touch Behavioral Response 2015 10 01 15 21 http://jbums.org/article-1-5278-en.pdf 10.22088/jbums.17.10.15
435-5361 2024-03-29 10.1002
Journal of Babol University of Medical Sciences J Babol Univ Med Sci 1561-4107 2251-7170 10.22088/jbums 2015 17 10 The Relationship between Level of Vitamin C in Follicular Fluid and ‎Maturation of Oocytes and Embryo Quality in Patients Undergoing In-vitro ‎Fertilization S Saffari MH Bahadori H Sharami P TorabZadeh M Goudarzvand BACKGROUND AND OBJECTIVE: Oxidative stress and its adverse effects in vitro or on the body can reduce the number of reproductive cells and embryo quality. Given the fact that vitamin C is a natural antioxidant with a protective role, in this study we aimed to evaluate the relationship between the level of vitamin C in follicular fluid (FF) and maturation of oocytes and embryo quality of patients undergoing in vitro fertilization (IVF). METHODS: This cross-sectional study was performed on the eggs and embryos of 50 patients admitted to IVF unit of Al Zahra Hospital, Rasht, Iran. Patients underwent the same mediations used to induce ovulation, and then they were injected 10000 units of human chorionic gonadotropin. Finally, they underwent 36 hours of follicle suction. Vitamin C level in FF was measured by biochemical methods. Maturation of oocytes and embryo quality were examined with inverted light microscope. FINDINGS: After examining 583 eggs and 275 embryos the following results were obtained: the percentage of metaphase II oocytes in vitamin C level of less than one was 81.3% (412), but when compared to vitamin C level of one or more, it was 71.1% (54), which were significantly different (p=0.038). In the sub-classification, vitamin C level (0.5-1 mg/dl), MII oocyte frequency and the two pronuclei embryos were higher but the difference was not statistically significant. CONCLUSION: The results showed that the quality of oocyte maturation and embryos in lower levels of vitamin C levels had improved, as compared to higher levels. Infertility In-vitro fertilization Levels of vitamin C Oocytes Follicular fluid Embryo quality Antioxidant 2015 10 01 22 27 http://jbums.org/article-1-5361-en.pdf 10.22088/jbums.17.10.22
435-5355 2024-03-29 10.1002
Journal of Babol University of Medical Sciences J Babol Univ Med Sci 1561-4107 2251-7170 10.22088/jbums 2015 17 10 Effects of Maternal Empowerment Program on the Length of ‎Hospitalization and Readmission Rate of Premature Infants S Karbandi A Momenizade M Heydarzade SR Mazlom M Hassanzade BACKGROUND AND OBJECTIVE: Premature infants because special needs might be admitted at the neonatal intensive care unit for days to recover. Despite medical advances, rate of readmission among preterm infants is relatively high, causing economic and psychological problems for families. This study aimed to evaluate the effects of empowerment Mother Program on length of stay and readmission rate of preterm infants. METHODS: This randomized clinical trial was conducted on 70 mothers with premature infants, divided into two groups of intervention and control. Educational-behavioral empowerment program was performed in four stages: 2-4 days after admission (stage one), 2-4 days after completing the first stage (stage two), 1-3 days before discharge (stage three), and one week after discharge (stage four). During each stage, mothers in the intervention group listened to selected audio files, and subjects in the control group received usual care based on hospital policies. In two stages, mothers were regularly enquired about the readmission rate of neonates via phone calls within 10-15 days after discharge. In addition, length of hospitalization was extracted from the medical records of the infants. IRCT:N201410151954. FINDINGS: In this study, mean of hospitalization in the intervention and control groups was 10.85±5.93 and 13.85±7.65 days, respectively (p=0.03). Moreover, no statistically significant difference was observed in the readmission rate of infants one month after discharge. CONCLUSION: According to the results of this study, the empowerment program caused a significant reduction in the length of hospital stay. However, it had no significant effects on the rate of readmission in the studied neonates Empowerment Program Length of Hospital Stay Readmission Rate Premature Infants‎ 2015 10 01 28 33 http://jbums.org/article-1-5355-en.pdf 10.22088/jbums.17.10.28
435-5346 2024-03-29 10.1002
Journal of Babol University of Medical Sciences J Babol Univ Med Sci 1561-4107 2251-7170 10.22088/jbums 2015 17 10 Associations of Oxidative Stress Indices in Infants Born via Natural ‎Delivery with Entonox Exposure K Vakilian dr.kvakilian@arakmu.ac.ir A Ranjbar BACKGROUND AND OBJECTIVE: Entonox is an odorless, tasteless gas and the first anesthesia agent used in obstetrics. To date, no studies have evaluated the effects of Entonox on oxidative stress. This study aimed to investigate the association between infant oxidative stress indices in mothers exposed to Entonox gas during vaginal delivery.  METHODS: This cohort study was conducted on 120 pregnant women undergoing natural labor. Subjects were divided into two groups of delivery with and without exposure to analgesic Entonox (N=60). After delivery, 5 ml of arterial blood was obtained from the umbilical cords of all infants, and indicators such as malondialdehyde, thiols and total antioxidant capacity were measured and evaluated in each group. FINDINGS: In this study, there were no significant differences in the indicators of oxidative stress between the study groups. Values obtained in groups with and without Entonox exposure were respectively as follows: total antioxidant capacity (1.33±1.21 vs. 1.59±1.3, p=0.84), thiols (0.363±0.313 vs. 0.238±0.225, p=0.07), lipid peroxidation (4.60±2.76 vs. 5.31±3.19, p=1). CONCLUSION: According to the results of this study, exposure to Entonox had no significant effects on the indicators of infant oxidative stress. Therefore, Entonox exposure could be used as a safe analgesic approach in natural delivery. Oxidative Stress Natural Delivery Painless Delivery Infant 2015 10 01 34 38 http://jbums.org/article-1-5346-en.pdf 10.22088/jbums.17.10.34
435-5349 2024-03-29 10.1002
Journal of Babol University of Medical Sciences J Babol Univ Med Sci 1561-4107 2251-7170 10.22088/jbums 2015 17 10 An Investigation of the Effect of Hypoxia on Expression of 107-miR in ‎Gastric Cancer Cell Lines MKN-45 and AGS‎ N Ayremlou H Mozdarani mozdarah@modares.ac.ir SJ Mowla A Delavari BACKGROUND AND OBJECTIVE: Hypoxia in solid tumors is the major cause of cancer treatment resistance. Thus, identifying the appropriate indicators of hypoxia in tumors is of great importance for appropriate tumor prognosis and choosing the best treatment methods. This study aims to investigate the modulation of miR-107 expression, as a biomarker of hypoxia and its association with gastric cancer cells. METHODS: MKN45 and AGS gastric cancer cells were purchased from Tehran Pasteur Institute and then, were cultured under normal oxygen conditions (CO2 5% and O2 95%) and hypoxia (CO2 5% and N2 95%). Finally, miR-107 and HIF-1-α expressions were evaluated every 24 to 48 hours. FINDINGS: The results of the study showed that through hypoxia induction, HIF-1-α expression in MKN-45 cell lines increased by extending treatment duration (24 and 48 hours) 2.3 and 3.8 times, respectively, (p=0.04 and p=0.002), and in AGS cell lines HIF-1-α expression increased 2.2 and 3.8 times (p=0.03 and p=0.005). In addition, miR-107 expression in MKN-45 increased during this time by 2.2 and 3.1 times (p=0.01 and p=0.0001), while in AGS cell lines it was by 2.4 and 4 times (p=0.002 and p=0.0001). CONCLUSION: It was demonstrated that hypoxia induction in gastric cancer cells could modulate the expression of miR-107.  Gastric cancer HIF-1-alpha Hypoxia miR-107‎ 2015 10 01 39 45 http://jbums.org/article-1-5349-en.pdf 10.22088/jbums.17.10.39
435-5326 2024-03-29 10.1002
Journal of Babol University of Medical Sciences J Babol Univ Med Sci 1561-4107 2251-7170 10.22088/jbums 2015 17 10 Early Detection of blaTEM in Klebsiella Isolates by the Molecular ‎Polymerase Chain Reaction Method S Bostandoost nikoo MH Shahhosseiny shahhosseiny@yahoo.com MR Zolfaghari M Rahbar BACKGROUND AND OBJECTIVE: Obtaining information regarding pathogenesis and prevalence of extended spectrum beta-lactamase (ESBL) producing genes seems to be necessary, since it can promote prevention modalities and treatment of the infections caused by bacterias such as Klebsiella. The aim of this study was early identification of the blaTEM gene in Klebsiella, using polymerase chain reaction (PCR) technique. METHODS: In this cross-sectional study, conducted form April to September 2013, 70 Klebsiella isolates were extracted from clinical samples (i.e., wound, urine, sputum and blood) using biochemical tests, including non-state fermentation and triple sugar iron, negative indole, motile and methyl red, as well as positive Voges–Proskauer and urease tests. Subsequently, the frequency of ESBL producing strains was determined by means of combined disk method. DNA was extracted by boiling and was investigated for the presence of TEM gene using the PCR approach. FINDINGS: In the 70 Klebsiella isolates, 11 cases of ESBL phenotype were observed, of which 10 cases contained TEM beta-lactamase resistance gene. In addition, 9 out of 59 samples (26%) of negative ESBL in antibiogram, were determined positive in terms of blaTEM gene using PCR method. CONCLUSION: Given the increasing prevalence of ESBL producing strains and poor diagnosis rate of antibiotic resistance through antibiogram method, applying more accurate techniques such as PCR is highly recommended. BlaTEM Resistance Gene Extended Spectrum Beta-lactamases Polymerase Chain Reaction Antibiogram 2015 10 01 46 52 http://jbums.org/article-1-5326-en.pdf 10.22088/jbums.17.10.46
435-4883 2024-03-29 10.1002
Journal of Babol University of Medical Sciences J Babol Univ Med Sci 1561-4107 2251-7170 10.22088/jbums 2015 17 10 A Comparison between the Molecular Identity of Mycoplasma Hominis in ‎Urine Samples of Patients with Urinary Tract Infections and Similar Strains ‎Available in GenBank H Eftekhari B kheirkhah b.kheirkhah@srbiau.ac.ir B Amirheydari BACKGROUND AND OBJECTIVE: Mycoplasmal infections are one of the most important urinary infections. Various studies have applied culture studies and biochemistry to separate the bacteria from the urinary tract. However, it should be noted that molecular and phylogenetic analyses are based on epidemiologic evaluations. The purpose of this study was to determine the molecular identity of Mycoplasma hominis, separated from the urine samples of patients with urinary tract infections in Kerman, Iran and compare the sequences with other strains in GenBank. METHODS: In this cross-sectional study, 5 ml mid-stream urine samples of 50 patients with urinary tract infections were collected. After the specialists confirmed the diagnosis of urinary tract infections in patients via paraclinical tests, segments of 16S rRNA gene were amplified, using specific primers of Mycoplasma hominis via polymerase chain reaction (PCR) technique. After purifying the PCR product, the sequences of bacterial strains were determined. Then, the sequences were aligned and the strains were compared with each other and other strains available in GenBank, using BioEdit software. FINDINGS: Three Mycoplasma hominis strains were separated in this study. The alignment of sequences and comparison with strains available in GenBank did not indicate a significant difference between the strains. Based on phylogenetic analyses in this study and the phylogenetic tree, one of the strains (H6) was highly similar to the strains of GenBank and belonged to the same family. On the other hand, two strains (H11 and H15) were of a different lineage and were completely different from other strains in the present study and those recorded in GenBank. CONCLUSION: In this study, after applying the PCR technique and bacterial separation, the sequences were compared with those in GenBank. All three strains were Mycoplasma hominis, based on 16S rRNA gene sequence. Mycoplasma hominis Urinary tract infection Polymerase chain reaction Nucleotide sequence 16S rRNA gene‎ 2015 10 01 53 59 http://jbums.org/article-1-4883-en.pdf 10.22088/jbums.17.10.53
435-5251 2024-03-29 10.1002
Journal of Babol University of Medical Sciences J Babol Univ Med Sci 1561-4107 2251-7170 10.22088/jbums 2015 17 10 A Comparative Study of the Effects of Sodium Arsenite and Nanoparticles ‎of Sodium Arsenite on the Apparent and Skeletal Malformations in Rat ‎Embryos H Njafzadeh Varzi najafzadeh@scu.ac.ir M Khaksari Mahabadi A Haji BACKGROUND AND OBJECTIVE: Arsenic causes congenital anomalies in humans and animals, and nanoparticles of sodium arsenite are highly capable of inducing apoptosis. Since the effects of nanoparticles of sodium arsenite on fetal malformations have not been evaluated yet, this study aims to compare the effect of sodium arsenite and nanoparticles of sodium arsenite on skeletal malformations in rat embryos. METHODS: This in-vitro study was performed on four groups of pregnant rats (n= 23 rats). Mating was confirmed by observation of vaginal plug. On the tenth day of gestation, pregnant rats in different groups received intraperitoneal normal saline (n=7 rats), sodium arsenite (11 mg/kg) (n=5 rats), nanoparticles of sodium arsenite (1 mg) (n=5 rats) and nanoparticles of sodium arsenite (11 mg) (n=7 rats). All the rats were euthanized on the twentieth day of pregnancy and their embryos were removed, their weight and length were measured, and then were stained with alizarin red and alcian blue. The skeletal system abnormities of embryos such as cleft palate, malfunctions or malformations in ribs, vertebrae, spine, sternum, arms, legs, fingers and reduction of ossification were evaluated by a stereomicroscope, and then compared with the control group. FINDINGS: Sodium arsenite and nanoparticles of sodium arsenite reduced fetal weight from 5 g in the control group to 2 g (p≤0.033) and also, decreased fetal length from 38 cm to 28 cm in the control group (p≤0.023). The weight and length of fetuses were significantly reduced in nanoparticles of sodium arsenite group (11 mg), as compared to sodium arsenite group (p≤0.033). There were no skeletal malformations in the control group, while the percentage of anomalities was between 3% and 47% in the intervention group. CONCLUSION: Nanoparticles of sodium arsenite, as compared to sodium arsenite, were more effective in reducing fetal length and weight and in diminishing the rate of skeletal malformations such as cleft palate Fetal Rats Skeletal Anomalies Sodium Arsenite 2015 10 01 60 66 http://jbums.org/article-1-5251-en.pdf 10.22088/jbums.17.10.60
435-5383 2024-03-29 10.1002
Journal of Babol University of Medical Sciences J Babol Univ Med Sci 1561-4107 2251-7170 10.22088/jbums 2015 17 10 Predicting Factors of Radiosensitivity in Individual Radiotherapy Sh ghasemi A Shabestani Monfared monfared_ali@yahoo.com S Borzoueisileh E Zabihi M Amiri S Abedian BACKGROUND AND OBJECTIVE: Radiation dose in oncology protocols is different for each patient according to the type and grade of the tumor, as well as adjuvant therapies. In the current treatment regimens, some predicting factors of individual radiosensitivity have not been considered. Individual radiotherapy can lower side effects through radiation dose reduction with respect to tumor control. In this study, the most determining factors for predicting radiosensitivity, used for individual radiotherapy, were reviewed. METHODS: Data were retrieved through searching Sciencedirect, PubMed, Google scholar, Iranmedex and SID databases. The titles and abstracts of Persian and English articles were searched using keywords including: radiotherapy, the rate of cell proliferation, tumoral hypoxia, inherent radiosensitivity, tumor cell cycle, inhibitor factors of tumor, cancer stem cells, field dose radiation, apoptosis and predicting factors of radiosensitivity. FINDINGS: Out of 90 articles, 25 original articles and reviews on predicting factors of the rate of radiosensitivity were thoroughly studied. Multiple factors, such as the presence of hypoxic zone and its size, inherent radiosensitivity and apoptosis, are crucial in determining individual radiation dose. Other factors, including previous history of exposure, blood type, left-or right-handedness and physical factors, should also be considered. CONCLUSION: With respect to the physical, chemical, and biological parameters influencing individual radiosensitivity, radiotherapy individualization can promote tumor treatment and diminish side effects of radiotherapy on normal tissue. Radiosensitivity Radiotherapy Radiation Dosage Side Effects 2015 10 01 67 73 http://jbums.org/article-1-5383-en.pdf 10.22088/jbums.17.10.67